diff --git a/assets/src/index.js b/assets/src/index.js
index 51e354442b..4994b81ac1 100644
--- a/assets/src/index.js
+++ b/assets/src/index.js
@@ -275,7 +275,9 @@ const initLizmapApp = () => {
         }
 
         // end waiting, does not depend on ongoing asynchronous actions
-        lizMap.waitEnd(startupConfigurations?.getFeatureInfo);
+        // startupFeatures is passed so highlights are re-applied after displayGetFeatureInfo clears them,
+        // even when the call is deferred until popup is initialized
+        lizMap.waitEnd(startupConfigurations?.getFeatureInfo, startupConfigurations?.startupFeatures);
 
         return;
     }, 'lizmap.class.loaded');
diff --git a/assets/src/legacy/map.js b/assets/src/legacy/map.js
index 749868ab92..fcf69d5ea6 100644
--- a/assets/src/legacy/map.js
+++ b/assets/src/legacy/map.js
@@ -3651,19 +3651,32 @@ window.lizMap = function() {
          * Wait end leads to interface modifications
          * Removes waiter and display getFeatureInfo, if requested
          * @param {Object} getFeatureInfo
+         * @param {Object} [startupFeatures] - GeoJSON startup features to re-highlight after popup display
          * @returns {void}
          */
-        waitEnd: (getFeatureInfo)=>{
+        waitEnd: (getFeatureInfo, startupFeatures)=>{
             $('body').css('cursor', 'auto');
             $('#loading').dialog('close');
 
             // Display getFeatureInfo if requested
             if(getFeatureInfo){
-                displayGetFeatureInfo(getFeatureInfo,
-                    {
-                        x: map.size.w / 2,
-                        y: map.size.h / 2
-                    });
+                const showGetFeatureInfo = () => {
+                    displayGetFeatureInfo(getFeatureInfo,
+                        {
+                            x: map.size.w / 2,
+                            y: map.size.h / 2
+                        });
+                    // Re-apply startup highlights cleared by popup display
+                    if (startupFeatures) {
+                        lizMap.mainLizmap.map.setHighlightFeatures(startupFeatures, "geojson");
+                    }
+                };
+                // popup is initialized after layers load — defer if not ready yet
+                if (lizMap.mainLizmap?.popup) {
+                    showGetFeatureInfo();
+                } else {
+                    lizMap.mainEventDispatcher.subscribe(showGetFeatureInfo, 'lizmap.modules.initialized');
+                }
             }
         },
         /**
diff --git a/tests/end2end/playwright/startup.spec.js b/tests/end2end/playwright/startup.spec.js
index b1e68fb92d..90aefb0c3a 100644
--- a/tests/end2end/playwright/startup.spec.js
+++ b/tests/end2end/playwright/startup.spec.js
@@ -41,6 +41,27 @@ test.describe('Startup', () => {
         await expect(page.locator('#lizmap-startup-features-error-message')).toHaveCount(1);
     });
 
+    test('Zoom to features extent and show popup with popup=true', async ({ page }) => {
+        const project = new ProjectPage(page, 'startup');
+        const getFeatureInfoPromise = project.waitForGetFeatureInfoRequest();
+        await project.openWithExtraParams({
+            'layer': 'sousquartiers',
+            'filter': '"quartmno" = \'PA\' OR "quartmno" = \'HO\'',
+            'popup': 'true',
+        });
+
+        // Wait for the GetFeatureInfo request triggered by popup=true
+        const getFeatureInfoRequest = await getFeatureInfoPromise;
+        const postData = getFeatureInfoRequest.postData() ?? '';
+        expect(postData).toContain('GetFeatureInfo');
+        expect(postData).toContain('sousquartiers');
+
+        // The popup dock must be visible with feature content
+        await expect(project.popupContent).toBeVisible();
+        const singleFeatures = await project.getPopupSingleFeatures();
+        await expect(singleFeatures).not.toHaveCount(0);
+    });
+
     test('Projects with dot or space can load', async ({ page }) => {
         let project = new ProjectPage(page, 'base_layers with space');
         await project.open();