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@@ -13,12 +13,12 @@ We aim to solve these challenges by developing a neuro-behavioral recording plat
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### Long-term dynamics of CA1 pyramidal neurons
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Using the above neuro-behavioral recording platform, we are working towards recording months-long, continuous neural activity across thousands of neurons while animals engage in complex behaviors across naturalistic environments. Data collected here will allow us and others to investigate neural activity at unprecedented scales and within the context of complex, unconstrained behavior. We believe this approach will shed light into the spaces between what traditional approaches have given us so far: producing lifelong “movies”, rather than individual “snapshots”, of neural dynamics and behavior across time, space, and task and allow us to ask "what does the lifetime of a place cell look like".
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### miniSTIM
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Great technological advances over the past decades have allowed researchers to begin to optically measure and modulate neural activity but these approaches are often limited to head-fix animals when studying neural function at spatial and temporal scales relevant to internal neural circuit dynamics. While a great deal of scientific and technological progress has been made, there is still much to learn concerning complex neural function, especially within the context of natural behavior. This gap in knowledge, at least in part, is due to a lack of accessible tools for simultaneously modulating and observing large-scale neural circuits with single cell precision in freely behaving animals. This project will fill this gap by developing open-source, head-mounted miniature microscopes with spatiotemporal illumination capabilities for both patterned photo-stimulation and improved neural imaging in freely behaving animals. To maximize the impact of our efforts, all tools and technologies developed for this project will be open-source and shared widely with the scientific community through online resources and technical workshops.
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### Spatio-Temporal Illumination Microscope (STIMscope)
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The study of neural dynamics, characterized by intricate spatial and temporal patterns, demands innovative approaches for comprehensive investigation. To address this need, we developed a novel, real-time closed-loop platform called the Spatio-Temporal Illumination Microscope (STIMscope). This system provides cellular-resolution, large field-of-view imaging and patterned stimulation in an open-source, customizable, and cost-effective package. A real-time synchronized control system enables seamless communication between all components, while custom firmware and a GPU-accelerated analysis pipeline ensure precise control and coordination. STIMscope’s versatile design supports a wide range of applications in neural imaging and neuromodulation, including studies in head-fixed mice and research in cell or tissue culture models.
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### miniSTIM
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Great technological advances over the past decades have allowed researchers to begin to optically measure and modulate neural activity but these approaches are often limited to head-fix animals when studying neural function at spatial and temporal scales relevant to internal neural circuit dynamics. While a great deal of scientific and technological progress has been made, there is still much to learn concerning complex neural function, especially within the context of natural behavior. This gap in knowledge, at least in part, is due to a lack of accessible tools for simultaneously modulating and observing large-scale neural circuits with single cell precision in freely behaving animals. This project will fill this gap by developing open-source, head-mounted miniature microscopes with spatiotemporal illumination capabilities for both patterned photo-stimulation and improved neural imaging in freely behaving animals. To maximize the impact of our efforts, all tools and technologies developed for this project will be open-source and shared widely with the scientific community through online resources and technical workshops.
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### Place cell dynamics across techniques and animal species
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As neural imaging techniques in freely behaving animals advance, there are still open questions as to the source of place cell property differences across imaging and electrophysiological techniques in different animal species. Using an array of Miniscope and ephsy devices, we are working towards uncovering these differences and their sources.
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