The study of neural dynamics, characterized by intricate spatial and temporal patterns, demands innovative approaches for comprehensive investigation. To address this need, we developed a novel, real-time closed-loop platform called the Spatio-Temporal Illumination Microscope (STIMscope). This system provides cellular-resolution, large field-of-view imaging and patterned stimulation in an open-source, customizable, and cost-effective package. A real-time synchronized control system enables seamless communication between all components, while custom firmware and a GPU-accelerated analysis pipeline ensure precise control and coordination. STIMscope’s versatile design supports a wide range of applications in neural imaging and neuromodulation, including studies in head-fixed mice and research in cell or tissue culture models.
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