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| 1 | +# 2D Part |
1 | 2 | # STEP 1: Install Anaconda |
2 | 3 | Download the executable from [this link](https://www.anaconda.com/download) then follow the instructions there to install Anaconda. |
3 | 4 |
|
@@ -39,3 +40,124 @@ Go to the next tab, the "Evaluate network" tab (6th tab). I recommend checking " |
39 | 40 | Now, you can move on to the "Analyze videos" tab (7th tab). The video should already be selected, but if it is not, you can re-add it. |
40 | 41 |
|
41 | 42 | :warning: **Make sure to check the "Save result(s) as csv" box**. Now you can click "Analyze Videos" and wait for it to complete. |
| 43 | + |
| 44 | +# 3D Part |
| 45 | +Now, repeat the above process for both of the 2d camera views, then you can generate the 3d data, using the following code: |
| 46 | +``` |
| 47 | +import deeplabcut |
| 48 | +import matplotlib |
| 49 | +config_path3d = '/home/nexus/Downloads/analyze_all_videos/DLC_3D_calibration/calibration-Susan-2022-09-01-3d/config.yaml' # edit this to your config path |
| 50 | +# if creating a new 3D calibration |
| 51 | +# config_path3d = deeplabcut.create_new_project_3d('calibration','Susan',num_cameras=2) |
| 52 | +# deeplabcut.calibrate_cameras(config_path3d, cbrow =5,cbcol =7,calibrate=False,alpha=0.9) |
| 53 | +# deeplabcut.calibrate_cameras(config_path3d, cbrow =5,cbcol =7,calibrate=True,alpha=0.9) |
| 54 | +# deeplabcut.check_undistortion(config_path3d, cbrow=5, cbcol=7) |
| 55 | +
|
| 56 | +video_path = '/home/nexus/Downloads/20230308/DLC_3D' |
| 57 | +
|
| 58 | +deeplabcut.triangulate(config_path3d,video_path,filterpredictions=True, videotype='mp4',save_as_csv =True) |
| 59 | +``` |
| 60 | + |
| 61 | +# Analysis of Data |
| 62 | +## STEP 1: Create a new Conda environment |
| 63 | +In an Anaconda terminal, run `conda create -n "mousearm-test python=3.11 -y"` or any environment name. |
| 64 | +Activate the environment with `conda activate mousearm-test`. |
| 65 | + |
| 66 | +## STEP 2: Install opensim via conda |
| 67 | +**Windows/Linux**: `conda install -c opensim-org opensim`. |
| 68 | +**macOS (Apple Silicon)**: `CONDA_SUBDIR=osx-64 conda install -c opensim-org opensim`. |
| 69 | + |
| 70 | +## STEP 3: Go to your Documents folder |
| 71 | +**Windows**: `cd %USERPROFILE%\Documents`. |
| 72 | +**Linux/macOS**: `cd ~/Documents`. |
| 73 | + |
| 74 | +## STEP 4: Clone this git repo and install the library |
| 75 | +Run the following in the Anaconda terminal: |
| 76 | +``` |
| 77 | +# Using SSH: |
| 78 | +git clone git@github.com:Al-Borno-Lab/mousearm.git |
| 79 | +
|
| 80 | +# OR using HTTPS: |
| 81 | +git clone https://github.com/Al-Borno-Lab/mousearm.git |
| 82 | +
|
| 83 | +cd mousearm |
| 84 | +pip install uv' |
| 85 | +
|
| 86 | +uv pip install -e . |
| 87 | +``` |
| 88 | + |
| 89 | +## STEP 5: Place your data folder anywhere with the following format (any number of reachsets) |
| 90 | +``` |
| 91 | +Data/ |
| 92 | +├── reachset_1/ |
| 93 | +│ └── kinematics_1.csv |
| 94 | +└── reachset_2/ |
| 95 | + └── kinematics_1.csv |
| 96 | +``` |
| 97 | +Then, in a separate anaconda terminal, cd into the parent directory of the Data folder. |
| 98 | + |
| 99 | +In a new python file in the same folder as the Data folder, enter: |
| 100 | +``` |
| 101 | +from mousearm.simulate import run_simulation |
| 102 | +run_simulation("Data", nReachSets=2) # Adjust nReachSets accordingly |
| 103 | +``` |
| 104 | +Then, run it using `python run ` from the parent directory of the Data folder. |
| 105 | + |
| 106 | +# Data Formatting |
| 107 | +Process the motion capture data through [DeepLabCut](https://github.com/DeepLabCut/DeepLabCut). The output file will have more data than necessary, so delete everything except time, paw (x,y,z), wrist (x,y,z), shoulder (x,y,z), elbow (x,y,z). At the end, the csv file should look like the following: |
| 108 | + |
| 109 | + |
| 110 | +After formatting, the data folder should be formatted like this: |
| 111 | +``` |
| 112 | +RawData/ |
| 113 | +└── reachsets/ |
| 114 | + ├── reachset_1/ |
| 115 | + │ └── kinematics_1.csv |
| 116 | + ├── reachset_2/ |
| 117 | + │ └── kinematics_1.csv |
| 118 | + └── ... |
| 119 | +``` |
| 120 | + |
| 121 | +# Output File Processing |
| 122 | + |
| 123 | +The important output files are muscle_solution and the muscle_kinematics: |
| 124 | +* muscle_solution shows the solution that the model found for the movement. These can be visualized in OpenSim to observe the movement and predicted muscle actuations. |
| 125 | +* muscle_kinematics shows the difference between the captured movement and the movement predicted by the model |
| 126 | + |
| 127 | +These files can both be imported into a pandas dataframe for further analysis with the below python code. The directory should be structured like this: |
| 128 | +``` |
| 129 | +RawData/ |
| 130 | +└── reachsets/ |
| 131 | + ├── reachset_1/ |
| 132 | + │ ├── muscle_solution_adjusted_kinematics_1.sto |
| 133 | + │ └── muscle_kinematics_adjusted_kinematics_1.csv |
| 134 | + ├── reachset_2/ |
| 135 | + │ ├── muscle_solution_adjusted_kinematics_1.sto |
| 136 | + │ └── muscle_kinematics_adjusted_kinematics_1.csv |
| 137 | + └── ... |
| 138 | +``` |
| 139 | +``` |
| 140 | +import pandas as pd |
| 141 | +import glob |
| 142 | +import numpy as np |
| 143 | +base_dir = "../../RawData/reachsets/" # Adjust this path to yours |
| 144 | +save_dir = "../../Data/" # Adjust this path to yours |
| 145 | +mcolnames = ["time", "/jointset/shoulder/elv_angle/value", "/jointset/shoulder/extension_angle/value", "/jointset/shoulder/rotation_angle/value", "/jointset/humerus_ulna/elbow_flex/value", "/jointset/ulna_radius_pj/radius_rot/value", "/jointset/wrist/wrist_angle/value", "/jointset/shoulder/elv_angle/speed", "/jointset/shoulder/extension_angle/speed", "/jointset/shoulder/rotation_angle/speed", "/jointset/humerus_ulna/elbow_flex/speed", "/jointset/ulna_radius_pj/radius_rot/speed", "/jointset/wrist/wrist_angle/speed", "/forceset/Pectoralis_Clavicle_Head/activation", "/forceset/Biceps_Short_Head/activation", "/forceset/Biceps_Long_Head/activation", "/forceset/Deltoid_Medial/activation", "/forceset/Triceps_Lat_Head/activation", "/forceset/Triceps_Long_Head/activation", "/forceset/Brachialis_Proximal_Head/activation", "/forceset/Brachialis_Distal_Head/activation", "/forceset/Anconeus/activation", "/forceset/Deltoid_Posterior/activation", "/forceset/Anconeus_Short_Head/activation", "/forceset/Subscapularis_SuperiorHead/activation", "/forceset/Infraspinatus/activation", "/forceset/PronatorTeres/activation", "/forceset/FlexorCarpiRadialis/activation", "/forceset/Brachioradialis/activation", "/forceset/Triceps_Medial_Head/activation", "/forceset/Latissimus_Dorsi_Rostral/activation", "/forceset/Latissimus_Dorsi_Caudal/activation", "/forceset/Pectoralis_Major_Anterior/activation", "/forceset/Pectoralis_Major_Posterior/activation", "/forceset/Pectoralis_Clavicle_Head", "/forceset/Biceps_Short_Head", "/forceset/Biceps_Long_Head", "/forceset/Deltoid_Medial", "/forceset/Triceps_Lat_Head", "/forceset/Triceps_Long_Head", "/forceset/Brachialis_Proximal_Head", "/forceset/Brachialis_Distal_Head", "/forceset/Anconeus", "/forceset/Deltoid_Posterior", "/forceset/Anconeus_Short_Head", "/forceset/Subscapularis_SuperiorHead", "/forceset/Infraspinatus", "/forceset/PronatorTeres", "/forceset/FlexorCarpiRadialis", "/forceset/Brachioradialis", "/forceset/Triceps_Medial_Head", "/forceset/Latissimus_Dorsi_Rostral", "/forceset/Latissimus_Dorsi_Caudal", "/forceset/Pectoralis_Major_Anterior", "/forceset/Pectoralis_Major_Posterior"] |
| 146 | +kcolnames = ["time","paw_x","paw_y","paw_z","elbow_x","elbow_y","elbow_z"] |
| 147 | +
|
| 148 | +def getMu(which_sets): |
| 149 | + mu = pd.DataFrame(); |
| 150 | + for set in which_sets: |
| 151 | + for file in glob.glob(base_dir+"reachset_"+str(set)+"/muscle_sol*"): |
| 152 | + tdf = pd.read_csv(file, sep= r'\t',engine='python', header=18, names=mcolnames, index_col=None) |
| 153 | + mu = pd.concat([mu,tdf], ignore_index=True) |
| 154 | + return mu |
| 155 | +
|
| 156 | +def getKin(which_sets): |
| 157 | + kin = pd.DataFrame(); |
| 158 | + for set in which_sets: |
| 159 | + for file in glob.glob(base_dir+"reachset_"+str(set)+"/muscle_kinematics_*"): |
| 160 | + tdf = pd.read_csv(file, sep= r',|\t',engine='python', header=4, names=kcolnames,index_col=None) |
| 161 | + kin = pd.concat([kin,tdf], ignore_index=True) |
| 162 | + return kin |
| 163 | +``` |
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