injection_procedures.md

Injection_procedures

Model definitions

Injection

Description of an injection procedure

Field	Type	Title (Description)
injection_materials	List[ViralMaterial or NonViralMaterial]	Injection material
targeted_structure	Optional[MouseAnatomyModel]	Injection target (Use InjectionTargets)
relative_position	Optional[List[AnatomicalRelative]]	Relative position
dynamics	List[InjectionDynamics]	Injection dynamics (List of injection events, one per location/depth)
protocol_id	Optional[str]	[DEPRECATED] Use protocol_id in Surgery or NonSurgicalInjection instead. Protocol ID (DOI for protocols.io)

InjectionDynamics

Description of the volume and rate of an injection

Field	Type	Title (Description)
profile	InjectionProfile	Injection profile
volume	Optional[float]	Injection volume
volume_unit	Optional[VolumeUnit]	Injection volume unit
duration	Optional[float]	Injection duration
duration_unit	Optional[TimeUnit]	Injection duration unit
injection_current	Optional[float]	Injection current (uA)
injection_current_unit	Optional[CurrentUnit]	Injection current unit
alternating_current	Optional[str]	Alternating current

InjectionProfile

Injection profile

Name	Value
BOLUS	Bolus
CONTINUOUS	Continuous
PULSED	Pulsed

NonViralMaterial

Description of a non-viral injection material

Field	Type	Title (Description)
concentration	Optional[float]	Concentration (Must provide concentration unit)
concentration_unit	Optional[str]	Concentration unit (For example, mg/mL)
name	str	Name
source	Organization	Source
rrid	Optional[PIDName]	Research Resource ID
lot_number	Optional[str]	Lot number
expiration_date	Optional[datetime.date]	Lot expiration date

TarsVirusIdentifiers

TARS data for a viral prep

Field	Type	Title (Description)
virus_tars_id	Optional[str]	Virus ID, usually begins 'AiV'
plasmid_tars_alias	Optional[List[str]]	List of plasmid aliases (Alias used to reference the plasmid, usually begins 'AiP')
prep_lot_number	str	Preparation lot number
prep_date	Optional[datetime.date]	Preparation lot date (Date this prep lot was titered)
prep_type	Optional[VirusPrepType]	Viral prep type
prep_protocol	Optional[str]	Prep protocol

ViralMaterial

Description of viral material for injections

Field	Type	Title (Description)
name	str	Full genome name (Full genome for virus construct)
tars_identifiers	Optional[TarsVirusIdentifiers]	TARS IDs (TARS database identifiers)
addgene_id	Optional[PIDName]	Addgene id (Registry must be Addgene)
titer	Optional[int]	Effective titer (Final titer of viral material, accounting for mixture/diliution)
titer_unit	Optional[str]	Titer unit (For example, gc/mL)

VirusPrepType

Type of virus preparation

Name	Value
CRUDE	Crude
PURIFIED	Purified