From 412cc59d698603cbc9cf0a27aa4816b396cc0077 Mon Sep 17 00:00:00 2001 From: Joshua Hernandez Date: Tue, 9 Dec 2025 22:28:18 -0500 Subject: [PATCH] add handle for first column containing the well --- .../qiacuity_dpcr/qiacuity_dpcr_reader.py | 10 +- .../qiacuity_dpcr/qiacuity_dpcr_structure.py | 25 +- .../testdata/qiacuity_dpcr_example04.csv | 34 + .../testdata/qiacuity_dpcr_example04.json | 1920 +++++++++++++++++ 4 files changed, 1981 insertions(+), 8 deletions(-) create mode 100644 tests/parsers/qiacuity_dpcr/testdata/qiacuity_dpcr_example04.csv create mode 100644 tests/parsers/qiacuity_dpcr/testdata/qiacuity_dpcr_example04.json diff --git a/src/allotropy/parsers/qiacuity_dpcr/qiacuity_dpcr_reader.py b/src/allotropy/parsers/qiacuity_dpcr/qiacuity_dpcr_reader.py index 57b05bcd29..698ac77673 100644 --- a/src/allotropy/parsers/qiacuity_dpcr/qiacuity_dpcr_reader.py +++ b/src/allotropy/parsers/qiacuity_dpcr/qiacuity_dpcr_reader.py @@ -19,8 +19,14 @@ def __init__(self, named_file_contents: NamedFileContents): qiacuity_dpcr_data.columns = qiacuity_dpcr_data.columns.str.replace("�", "μ") qiacuity_dpcr_data.columns = qiacuity_dpcr_data.columns.str.replace("Âμ", "μ") column_names = qiacuity_dpcr_data.columns.tolist() - # Rename the blank column to specify that it's the Well Name column - column_names[0] = "Well Name" + if "Well" in column_names: + # Rename "Well" to "Well Name" for consistency + column_names = [ + "Well Name" if col == "Well" else col for col in column_names + ] + else: + # Rename the blank first column to specify that it's the Well Name column + column_names[0] = "Well Name" column_index = pd.Index(column_names) qiacuity_dpcr_data.columns = column_index self.well_data = qiacuity_dpcr_data diff --git a/src/allotropy/parsers/qiacuity_dpcr/qiacuity_dpcr_structure.py b/src/allotropy/parsers/qiacuity_dpcr/qiacuity_dpcr_structure.py index ee1aa41182..3472d13539 100644 --- a/src/allotropy/parsers/qiacuity_dpcr/qiacuity_dpcr_structure.py +++ b/src/allotropy/parsers/qiacuity_dpcr/qiacuity_dpcr_structure.py @@ -31,7 +31,9 @@ def create_measurements(data: SeriesData) -> Measurement: identifier = data.get(str, "_measurement_identifier") or random_uuid_str() - sample_custom_info = data.get_custom_keys({"IC", "Control type"}) + sample_custom_info = data.get_custom_keys( + {"IC", "Control type", "Target (IC)", "Target (Control)"} + ) for key in sample_custom_info: if sample_custom_info[key] in ("", "-", "-", "--"): sample_custom_info[key] = None @@ -43,11 +45,22 @@ def create_measurements(data: SeriesData) -> Measurement: sample_role_type=sample_role_type, location_identifier=data[str, "Well Name"], plate_identifier=data.get(str, "Plate ID"), - target_identifier=data[str, "Target"], - total_partition_count=data[int, "Partitions (valid)"], - concentration=data[float, ["Concentration (copies/μL)", "Conc. [copies/μL]"]], - positive_partition_count=data[int, "Partitions (positive)"], - negative_partition_count=data.get(int, "Partitions (negative)"), + target_identifier=data[str, ["Target", "Target (Name)"]], + total_partition_count=data[int, ["Partitions (valid)", "Partitions (Valid)"]], + concentration=data[ + float, + [ + "Concentration (copies/μL)", + "Conc. [copies/μL]", + "Conc. [cp/μL] (dPCR reaction)", + ], + ], + positive_partition_count=data[ + int, ["Partitions (positive)", "Partitions (Positive)"] + ], + negative_partition_count=data.get( + int, ["Partitions (negative)", "Partitions (Negative)"] + ), fluorescence_intensity_threshold_setting=data.get(float, "Threshold"), sample_custom_info=sample_custom_info, custom_info=data.get_unread(), diff --git a/tests/parsers/qiacuity_dpcr/testdata/qiacuity_dpcr_example04.csv b/tests/parsers/qiacuity_dpcr/testdata/qiacuity_dpcr_example04.csv new file mode 100644 index 0000000000..de82446972 --- /dev/null +++ b/tests/parsers/qiacuity_dpcr/testdata/qiacuity_dpcr_example04.csv @@ -0,0 +1,34 @@ +sep=, +"Plate name","Plate ID","Plate type","Well","Sample/NTC/Control","Type","Reaction Mix","Target (Name)","Target (IC)","Target (Control)","Conc. [cp/µL] (dPCR reaction)","Conc. [cp/mL] (dPCR reaction)","CI (95%) (dPCR reaction)","Conc. [cp/µL] (undiluted sample)","Conc. [cp/mL] (undiluted sample)","Partitions (Valid)","Partitions (Positive)","Partitions (Negative)","Threshold","Volume per well [µl]","Concentration factor","Template volume [µl]","Total volume [µl]","Conversion factor","Conversion unit" +"250227 AAV MA 6","2186f026-541d-438c-8195-2a1da8a81e56","Nanoplate 8.5K 96-well","A1","1_5000","Sample","eGFP","eGFP","-","-","7782.4","7782381.203592551","2.9%","1686182594.1","1686182594111.7194","8261","7644","617","19.45","2.837","50000","3.6","15.6","1000","cp/mL" +"","","","A2","1_5000","Sample","eGFP","eGFP","-","-","8243.3","8243312.142179277","3.0%","1786050964.1","1786050964138.843","8220","7693","527","19.45","2.836","50000","3.6","15.6","1000","cp/mL" +"","","","A3","1_10000","Sample","eGFP","eGFP","-","-","4331.8","4331802.288772191","2.7%","1877114325.1","1877114325134.616","8251","6314","1937","19.45","2.847","100000","3.6","15.6","1000","cp/mL" +"","","","A4","1_10000","Sample","eGFP","eGFP","-","-","4131.6","4131574.801895133","2.7%","1790349080.8","1790349080821.2242","8258","6179","2079","19.45","2.841","100000","3.6","15.6","1000","cp/mL" +"","","","A5","GFP+_10000","Sample","eGFP","eGFP","-","-","4273.2","4273167.253847267","2.7%","185170581.0","185170581000.04822","8258","6262","1996","19.45","2.828","10000","3.6","15.6","1000","cp/mL" +"","","","B1","2_5000","Sample","eGFP","eGFP","-","-","7293.9","7293931.173215276","2.9%","1580351754.2","1580351754196.643","8268","7545","723","19.45","2.843","50000","3.6","15.6","1000","cp/mL" +"","","","B2","2_5000","Sample","eGFP","eGFP","-","-","7501.9","7501877.055196923","2.9%","1625406695.3","1625406695292.6664","8256","7539","717","19.45","2.772","50000","3.6","15.6","1000","cp/mL" +"","","","B3","2_10000","Sample","eGFP","eGFP","-","-","3881.2","3881217.559902232","2.7%","1681860942.6","1681860942624.3005","8265","5922","2343","19.45","2.764","100000","3.6","15.6","1000","cp/mL" +"","","","B4","2_10000","Sample","eGFP","eGFP","-","-","3841.0","3841025.99623931","2.7%","1664444598.4","1664444598370.3673","8263","5840","2423","19.45","2.718","100000","3.6","15.6","1000","cp/mL" +"","","","B5","GFP+_10000","Sample","eGFP","eGFP","-","-","4308.0","4307999.978497452","2.7%","186679999.1","186679999068.2229","8263","6181","2082","19.45","2.723","10000","3.6","15.6","1000","cp/mL" +"","","","C1","3_5000","Sample","eGFP","eGFP","-","-","8421.5","8421468.976341419","3.0%","1824651611.5","1824651611540.6408","8257","7738","519","19.45","2.796","50000","3.6","15.6","1000","cp/mL" +"","","","C2","3_5000","Sample","eGFP","eGFP","-","-","8345.5","8345452.807031626","3.0%","1808181441.5","1808181441523.5188","8268","7686","582","19.45","2.706","50000","3.6","15.6","1000","cp/mL" +"","","","C3","3_10000","Sample","eGFP","eGFP","-","-","4496.9","4496934.671644322","2.7%","1948671691.0","1948671691045.873","8281","6296","1985","19.45","2.703","100000","3.6","15.6","1000","cp/mL" +"","","","C4","3_10000","Sample","eGFP","eGFP","-","-","4286.8","4286843.191831076","2.7%","1857632049.8","1857632049793.4663","8280","6114","2166","19.45","2.662","100000","3.6","15.6","1000","cp/mL" +"","","","C5","GFP+_10000","Sample","eGFP","eGFP","-","-","4460.6","4460641.102538355","2.7%","193294447.8","193294447776.66205","8257","6193","2064","19.45","2.645","10000","3.6","15.6","1000","cp/mL" +"","","","D1","4_5000","Sample","eGFP","eGFP","-","-","7045.4","7045441.152649994","2.8%","1526512249.7","1526512249740.832","8285","7476","809","19.45","2.81","50000","3.6","15.6","1000","cp/mL" +"","","","D2","4_5000","Sample","eGFP","eGFP","-","-","7069.1","7069057.464728602","2.8%","1531629117.4","1531629117357.8637","8278","7418","860","19.45","2.726","50000","3.6","15.6","1000","cp/mL" +"","","","D3","4_10000","Sample","eGFP","eGFP","-","-","3853.2","3853186.510478793","2.7%","1669714154.5","1669714154540.8099","8257","5821","2436","19.45","2.696","100000","3.6","15.6","1000","cp/mL" +"","","","D4","4_10000","Sample","eGFP","eGFP","-","-","3657.5","3657500.634484687","2.8%","1584916941.6","1584916941610.0307","8276","5640","2636","19.45","2.662","100000","3.6","15.6","1000","cp/mL" +"","","","D5","GFP+_10000","Sample","eGFP","eGFP","-","-","4623.5","4623482.543713903","2.7%","200350910.2","200350910227.60248","8269","6251","2018","19.45","2.596","10000","3.6","15.6","1000","cp/mL" +"","","","E1","5_5000","Sample","eGFP","eGFP","-","-","6629.6","6629628.363322401","2.8%","1436419478.7","1436419478719.8536","8274","7345","929","19.45","2.807","50000","3.6","15.6","1000","cp/mL" +"","","","E2","5_5000","Sample","eGFP","eGFP","-","-","6718.0","6718029.225576681","2.8%","1455572998.9","1455572998874.9473","8275","7313","962","19.45","2.726","50000","3.6","15.6","1000","cp/mL" +"","","","E3","5_10000","Sample","eGFP","eGFP","-","-","3537.6","3537560.946733544","2.8%","1532943076.9","1532943076917.8689","8263","5510","2753","19.45","2.644","100000","3.6","15.6","1000","cp/mL" +"","","","E4","5_10000","Sample","eGFP","eGFP","-","-","3386.7","3386703.056941245","2.8%","1467571324.7","1467571324674.5393","8264","5398","2866","19.45","2.661","100000","3.6","15.6","1000","cp/mL" +"","","","E5","NTC_1","Sample","eGFP","eGFP","-","-","0.000","0.000","-","0.000","0.000","8263","0","8263","19.45","2.621","1","3.6","15.6","1000","cp/mL" +"","","","F1","6_5000","Sample","eGFP","eGFP","-","-","6685.3","6685344.814481614","2.8%","1448491376.5","1448491376471.0164","8259","7350","909","19.45","2.809","50000","3.6","15.6","1000","cp/mL" +"","","","F2","6_5000","Sample","eGFP","eGFP","-","-","6638.5","6638452.811055944","2.8%","1438331442.4","1438331442395.4546","8247","7287","960","19.45","2.757","50000","3.6","15.6","1000","cp/mL" +"","","","F3","6_10000","Sample","eGFP","eGFP","-","-","3545.9","3545949.866982259","2.8%","1536578275.7","1536578275692.3122","8249","5601","2648","19.45","2.727","100000","3.6","15.6","1000","cp/mL" +"","","","F4","6_10000","Sample","eGFP","eGFP","-","-","3456.3","3456298.662462109","2.8%","1497729420.4","1497729420400.2473","8212","5382","2830","19.45","2.623","100000","3.6","15.6","1000","cp/mL" +"","","","F5","NTC_1","Sample","eGFP","eGFP","-","-","0.000","0.000","-","0.000","0.000","6287","0","6287","19.45","2.639","1","3.6","15.6","1000","cp/mL" +"","","","G5","NTC_1","Sample","eGFP","eGFP","-","-","0.386","385.539138675","147.5%","1.671","1670.669600924","8249","1","8248","19.45","2.676","1","3.6","15.6","1000","cp/mL" +"","","","H5","NTC_1","Sample","eGFP","eGFP","-","-","0.000","0.000","-","0.000","0.000","8211","0","8211","19.45","2.734","1","3.6","15.6","1000","cp/mL" \ No newline at end of file diff --git a/tests/parsers/qiacuity_dpcr/testdata/qiacuity_dpcr_example04.json b/tests/parsers/qiacuity_dpcr/testdata/qiacuity_dpcr_example04.json new file mode 100644 index 0000000000..c3a9a49be6 --- /dev/null +++ b/tests/parsers/qiacuity_dpcr/testdata/qiacuity_dpcr_example04.json @@ -0,0 +1,1920 @@ +{ + "$asm.manifest": "http://purl.allotrope.org/manifests/pcr/BENCHLING/2023/09/dpcr.manifest", + "dPCR aggregate document": { + "device system document": { + "device identifier": "Qiacuity dPCR", + "brand name": "Qiacuity Digital PCR System", + "product manufacturer": "Qiagen" + }, + "dPCR document": [ + { + "measurement aggregate document": { + "plate well count": { + "value": 0, + "unit": "#" + }, + "measurement document": [ + { + "measurement identifier": "QIACUITY_DPCR_TEST_ID_0", + "measurement time": "1970-01-01T00:00:00+00:00", + "target DNA description": "eGFP", + "sample document": { + "sample identifier": "1_5000", + "sample role type": "Sample Role", + "well location identifier": "A1", + "well plate identifier": "2186f026-541d-438c-8195-2a1da8a81e56" + }, + "device control aggregate document": { + "device control document": [ + { + "device type": "dPCR", + "device identifier": "Qiacuity dPCR" + } + ] + }, + "total partition count": { + "value": 8261, + "unit": "#" + }, + "processed data aggregate document": { + "processed data document": [ + { + "number concentration": { + "value": 7782.4, + "unit": "#/μL" + }, + "positive partition count": { + "value": 7644, + "unit": "#" + }, + "data processing document": { + "fluorescence intensity threshold setting": { + "value": 19.45, + "unit": "(unitless)" + } + }, + "negative partition count": { + "value": 617, + "unit": "#" + } + } + ] + }, + "custom information document": { + "CI (95%) (dPCR reaction)": 2.9, + "Concentration factor": 50000.0, + "Template volume [μl]": 3.6, + "Plate name": "250227 AAV MA 6", + "Conc. [cp/mL] (undiluted sample)": 1686182594111.7195, + "Reaction Mix": "eGFP", + "Volume per well [μl]": 2.837, + "Conversion unit": "cp/mL", + "Total volume [μl]": 15.6, + "Conc. [cp/mL] (dPCR reaction)": 7782381.203592551, + "Plate type": "Nanoplate 8.5K 96-well", + "Conversion factor": 1000.0, + "Conc. [cp/μL] (undiluted sample)": 1686182594.1 + } + }, + { + "measurement identifier": "QIACUITY_DPCR_TEST_ID_1", + "measurement time": "1970-01-01T00:00:00+00:00", + "target DNA description": "eGFP", + "sample document": { + "sample identifier": "1_5000", + "sample role type": "Sample Role", + "well location identifier": "A2" + }, + "device control aggregate document": { + "device control document": [ + { + "device type": "dPCR", + "device identifier": "Qiacuity dPCR" + } + ] + }, + "total partition count": { + "value": 8220, + "unit": "#" + }, + "processed data aggregate document": { + "processed data document": [ + { + "number concentration": { + "value": 8243.3, + "unit": "#/μL" + }, + "positive partition count": { + "value": 7693, + "unit": "#" + }, + "data processing document": { + "fluorescence intensity threshold setting": { + "value": 19.45, + "unit": "(unitless)" + } + }, + "negative partition count": { + "value": 527, + "unit": "#" + } + } + ] + }, + "custom information document": { + "CI (95%) (dPCR reaction)": 3.0, + "Concentration factor": 50000.0, + "Template volume [μl]": 3.6, + "Conc. [cp/mL] (undiluted sample)": 1786050964138.843, + "Reaction Mix": "eGFP", + "Volume per well [μl]": 2.836, + "Conversion unit": "cp/mL", + "Total volume [μl]": 15.6, + "Conc. [cp/mL] (dPCR reaction)": 8243312.142179277, + "Conversion factor": 1000.0, + "Conc. [cp/μL] (undiluted sample)": 1786050964.1 + } + }, + { + "measurement identifier": "QIACUITY_DPCR_TEST_ID_2", + "measurement time": "1970-01-01T00:00:00+00:00", + "target DNA description": "eGFP", + "sample document": { + "sample identifier": "1_10000", + "sample role type": "Sample Role", + "well location identifier": "A3" + }, + "device control aggregate document": { + "device control document": [ + { + "device type": "dPCR", + "device identifier": "Qiacuity dPCR" + } + ] + }, + "total partition count": { + "value": 8251, + "unit": "#" + }, + "processed data aggregate document": { + "processed data document": [ + { + "number concentration": { + "value": 4331.8, + "unit": "#/μL" + }, + "positive partition count": { + "value": 6314, + "unit": "#" + }, + "data processing document": { + "fluorescence intensity threshold setting": { + "value": 19.45, + "unit": "(unitless)" + } + }, + "negative partition count": { + "value": 1937, + "unit": "#" + } + } + ] + }, + "custom information document": { + "CI (95%) (dPCR reaction)": 2.7, + "Concentration factor": 100000.0, + "Template volume [μl]": 3.6, + "Conc. [cp/mL] (undiluted sample)": 1877114325134.616, + "Reaction Mix": "eGFP", + "Volume per well [μl]": 2.847, + "Conversion unit": "cp/mL", + "Total volume [μl]": 15.6, + "Conc. [cp/mL] (dPCR reaction)": 4331802.288772191, + "Conversion factor": 1000.0, + "Conc. [cp/μL] (undiluted sample)": 1877114325.1 + } + }, + { + "measurement identifier": "QIACUITY_DPCR_TEST_ID_3", + "measurement time": "1970-01-01T00:00:00+00:00", + "target DNA description": "eGFP", + "sample document": { + "sample identifier": "1_10000", + "sample role type": "Sample Role", + "well location identifier": "A4" + }, + "device control aggregate document": { + "device control document": [ + { + "device type": "dPCR", + "device identifier": "Qiacuity dPCR" + } + ] + }, + "total partition count": { + "value": 8258, + "unit": "#" + }, + "processed data aggregate document": { + "processed data document": [ + { + "number concentration": { + "value": 4131.6, + "unit": "#/μL" + }, + "positive partition count": { + "value": 6179, + "unit": "#" + }, + "data processing document": { + "fluorescence intensity threshold setting": { + "value": 19.45, + "unit": "(unitless)" + } + }, + "negative partition count": { + "value": 2079, + "unit": "#" + } + } + ] + }, + "custom information document": { + "CI (95%) (dPCR reaction)": 2.7, + "Concentration factor": 100000.0, + "Template volume [μl]": 3.6, + "Conc. [cp/mL] (undiluted sample)": 1790349080821.224, + "Reaction Mix": "eGFP", + "Volume per well [μl]": 2.841, + "Conversion unit": "cp/mL", + "Total volume [μl]": 15.6, + "Conc. [cp/mL] (dPCR reaction)": 4131574.801895133, + "Conversion factor": 1000.0, + "Conc. [cp/μL] (undiluted sample)": 1790349080.8 + } + }, + { + "measurement identifier": "QIACUITY_DPCR_TEST_ID_4", + "measurement time": "1970-01-01T00:00:00+00:00", + "target DNA description": "eGFP", + "sample document": { + "sample identifier": "GFP+_10000", + "sample role type": "Sample Role", + "well location identifier": "A5" + }, + "device control aggregate document": { + "device control document": [ + { + "device type": "dPCR", + "device identifier": "Qiacuity dPCR" + } + ] + }, + "total partition count": { + "value": 8258, + "unit": "#" + }, + "processed data aggregate document": { + "processed data document": [ + { + "number concentration": { + "value": 4273.2, + "unit": "#/μL" + }, + "positive partition count": { + "value": 6262, + "unit": "#" + }, + "data processing document": { + "fluorescence intensity threshold setting": { + "value": 19.45, + "unit": "(unitless)" + } + }, + "negative partition count": { + "value": 1996, + "unit": "#" + } + } + ] + }, + "custom information document": { + "CI (95%) (dPCR reaction)": 2.7, + "Concentration factor": 10000.0, + "Template volume [μl]": 3.6, + "Conc. [cp/mL] (undiluted sample)": 185170581000.04825, + "Reaction Mix": "eGFP", + "Volume per well [μl]": 2.828, + "Conversion unit": "cp/mL", + "Total volume [μl]": 15.6, + "Conc. [cp/mL] (dPCR reaction)": 4273167.253847267, + "Conversion factor": 1000.0, + "Conc. [cp/μL] (undiluted sample)": 185170581.0 + } + }, + { + "measurement identifier": "QIACUITY_DPCR_TEST_ID_5", + "measurement time": "1970-01-01T00:00:00+00:00", + "target DNA description": "eGFP", + "sample document": { + "sample identifier": "2_5000", + "sample role type": "Sample Role", + "well location identifier": "B1" + }, + "device control aggregate document": { + "device control document": [ + { + "device type": "dPCR", + "device identifier": "Qiacuity dPCR" + } + ] + }, + "total partition count": { + "value": 8268, + "unit": "#" + }, + "processed data aggregate document": { + "processed data document": [ + { + "number concentration": { + "value": 7293.9, + "unit": "#/μL" + }, + "positive partition count": { + "value": 7545, + "unit": "#" + }, + "data processing document": { + "fluorescence intensity threshold setting": { + "value": 19.45, + "unit": "(unitless)" + } + }, + "negative partition count": { + "value": 723, + "unit": "#" + } + } + ] + }, + "custom information document": { + "CI (95%) (dPCR reaction)": 2.9, + "Concentration factor": 50000.0, + "Template volume [μl]": 3.6, + "Conc. [cp/mL] (undiluted sample)": 1580351754196.643, + "Reaction Mix": "eGFP", + "Volume per well [μl]": 2.843, + "Conversion unit": "cp/mL", + "Total volume [μl]": 15.6, + "Conc. [cp/mL] (dPCR reaction)": 7293931.173215276, + "Conversion factor": 1000.0, + "Conc. [cp/μL] (undiluted sample)": 1580351754.2 + } + }, + { + "measurement identifier": "QIACUITY_DPCR_TEST_ID_6", + "measurement time": "1970-01-01T00:00:00+00:00", + "target DNA description": "eGFP", + "sample document": { + "sample identifier": "2_5000", + "sample role type": "Sample Role", + "well location identifier": "B2" + }, + "device control aggregate document": { + "device control document": [ + { + "device type": "dPCR", + "device identifier": "Qiacuity dPCR" + } + ] + }, + "total partition count": { + "value": 8256, + "unit": "#" + }, + "processed data aggregate document": { + "processed data document": [ + { + "number concentration": { + "value": 7501.9, + "unit": "#/μL" + }, + "positive partition count": { + "value": 7539, + "unit": "#" + }, + "data processing document": { + "fluorescence intensity threshold setting": { + "value": 19.45, + "unit": "(unitless)" + } + }, + "negative partition count": { + "value": 717, + "unit": "#" + } + } + ] + }, + "custom information document": { + "CI (95%) (dPCR reaction)": 2.9, + "Concentration factor": 50000.0, + "Template volume [μl]": 3.6, + "Conc. [cp/mL] (undiluted sample)": 1625406695292.6665, + "Reaction Mix": "eGFP", + "Volume per well [μl]": 2.772, + "Conversion unit": "cp/mL", + "Total volume [μl]": 15.6, + "Conc. [cp/mL] (dPCR reaction)": 7501877.055196923, + "Conversion factor": 1000.0, + "Conc. [cp/μL] (undiluted sample)": 1625406695.3 + } + }, + { + "measurement identifier": "QIACUITY_DPCR_TEST_ID_7", + "measurement time": "1970-01-01T00:00:00+00:00", + "target DNA description": "eGFP", + "sample document": { + "sample identifier": "2_10000", + "sample role type": "Sample Role", + "well location identifier": "B3" + }, + "device control aggregate document": { + "device control document": [ + { + "device type": "dPCR", + "device identifier": "Qiacuity dPCR" + } + ] + }, + "total partition count": { + "value": 8265, + "unit": "#" + }, + "processed data aggregate document": { + "processed data document": [ + { + "number concentration": { + "value": 3881.2, + "unit": "#/μL" + }, + "positive partition count": { + "value": 5922, + "unit": "#" + }, + "data processing document": { + "fluorescence intensity threshold setting": { + "value": 19.45, + "unit": "(unitless)" + } + }, + "negative partition count": { + "value": 2343, + "unit": "#" + } + } + ] + }, + "custom information document": { + "CI (95%) (dPCR reaction)": 2.7, + "Concentration factor": 100000.0, + "Template volume [μl]": 3.6, + "Conc. [cp/mL] (undiluted sample)": 1681860942624.3003, + "Reaction Mix": "eGFP", + "Volume per well [μl]": 2.764, + "Conversion unit": "cp/mL", + "Total volume [μl]": 15.6, + "Conc. [cp/mL] (dPCR reaction)": 3881217.559902232, + "Conversion factor": 1000.0, + "Conc. [cp/μL] (undiluted sample)": 1681860942.6 + } + }, + { + "measurement identifier": "QIACUITY_DPCR_TEST_ID_8", + "measurement time": "1970-01-01T00:00:00+00:00", + "target DNA description": "eGFP", + "sample document": { + "sample identifier": "2_10000", + "sample role type": "Sample Role", + "well location identifier": "B4" + }, + "device control aggregate document": { + "device control document": [ + { + "device type": "dPCR", + "device identifier": "Qiacuity dPCR" + } + ] + }, + "total partition count": { + "value": 8263, + "unit": "#" + }, + "processed data aggregate document": { + "processed data document": [ + { + "number concentration": { + "value": 3841.0, + "unit": "#/μL" + }, + "positive partition count": { + "value": 5840, + "unit": "#" + }, + "data processing document": { + "fluorescence intensity threshold setting": { + "value": 19.45, + "unit": "(unitless)" + } + }, + "negative partition count": { + "value": 2423, + "unit": "#" + } + } + ] + }, + "custom information document": { + "CI (95%) (dPCR reaction)": 2.7, + "Concentration factor": 100000.0, + "Template volume [μl]": 3.6, + "Conc. [cp/mL] (undiluted sample)": 1664444598370.3672, + "Reaction Mix": "eGFP", + "Volume per well [μl]": 2.718, + "Conversion unit": "cp/mL", + "Total volume [μl]": 15.6, + "Conc. [cp/mL] (dPCR reaction)": 3841025.99623931, + "Conversion factor": 1000.0, + "Conc. [cp/μL] (undiluted sample)": 1664444598.4 + } + }, + { + "measurement identifier": "QIACUITY_DPCR_TEST_ID_9", + "measurement time": "1970-01-01T00:00:00+00:00", + "target DNA description": "eGFP", + "sample document": { + "sample identifier": "GFP+_10000", + "sample role type": "Sample Role", + "well location identifier": "B5" + }, + "device control aggregate document": { + "device control document": [ + { + "device type": "dPCR", + "device identifier": "Qiacuity dPCR" + } + ] + }, + "total partition count": { + "value": 8263, + "unit": "#" + }, + "processed data aggregate document": { + "processed data document": [ + { + "number concentration": { + "value": 4308.0, + "unit": "#/μL" + }, + "positive partition count": { + "value": 6181, + "unit": "#" + }, + "data processing document": { + "fluorescence intensity threshold setting": { + "value": 19.45, + "unit": "(unitless)" + } + }, + "negative partition count": { + "value": 2082, + "unit": "#" + } + } + ] + }, + "custom information document": { + "CI (95%) (dPCR reaction)": 2.7, + "Concentration factor": 10000.0, + "Template volume [μl]": 3.6, + "Conc. [cp/mL] (undiluted sample)": 186679999068.2229, + "Reaction Mix": "eGFP", + "Volume per well [μl]": 2.723, + "Conversion unit": "cp/mL", + "Total volume [μl]": 15.6, + "Conc. [cp/mL] (dPCR reaction)": 4307999.978497452, + "Conversion factor": 1000.0, + "Conc. [cp/μL] (undiluted sample)": 186679999.1 + } + }, + { + "measurement identifier": "QIACUITY_DPCR_TEST_ID_10", + "measurement time": "1970-01-01T00:00:00+00:00", + "target DNA description": "eGFP", + "sample document": { + "sample identifier": "3_5000", + "sample role type": "Sample Role", + "well location identifier": "C1" + }, + "device control aggregate document": { + "device control document": [ + { + "device type": "dPCR", + "device identifier": "Qiacuity dPCR" + } + ] + }, + "total partition count": { + "value": 8257, + "unit": "#" + }, + "processed data aggregate document": { + "processed data document": [ + { + "number concentration": { + "value": 8421.5, + "unit": "#/μL" + }, + "positive partition count": { + "value": 7738, + "unit": "#" + }, + "data processing document": { + "fluorescence intensity threshold setting": { + "value": 19.45, + "unit": "(unitless)" + } + }, + "negative partition count": { + "value": 519, + "unit": "#" + } + } + ] + }, + "custom information document": { + "CI (95%) (dPCR reaction)": 3.0, + "Concentration factor": 50000.0, + "Template volume [μl]": 3.6, + "Conc. [cp/mL] (undiluted sample)": 1824651611540.6409, + "Reaction Mix": "eGFP", + "Volume per well [μl]": 2.796, + "Conversion unit": "cp/mL", + "Total volume [μl]": 15.6, + "Conc. [cp/mL] (dPCR reaction)": 8421468.976341419, + "Conversion factor": 1000.0, + "Conc. [cp/μL] (undiluted sample)": 1824651611.5 + } + }, + { + "measurement identifier": "QIACUITY_DPCR_TEST_ID_11", + "measurement time": "1970-01-01T00:00:00+00:00", + "target DNA description": "eGFP", + "sample document": { + "sample identifier": "3_5000", + "sample role type": "Sample Role", + "well location identifier": "C2" + }, + "device control aggregate document": { + "device control document": [ + { + "device type": "dPCR", + "device identifier": "Qiacuity dPCR" + } + ] + }, + "total partition count": { + "value": 8268, + "unit": "#" + }, + "processed data aggregate document": { + "processed data document": [ + { + "number concentration": { + "value": 8345.5, + "unit": "#/μL" + }, + "positive partition count": { + "value": 7686, + "unit": "#" + }, + "data processing document": { + "fluorescence intensity threshold setting": { + "value": 19.45, + "unit": "(unitless)" + } + }, + "negative partition count": { + "value": 582, + "unit": "#" + } + } + ] + }, + "custom information document": { + "CI (95%) (dPCR reaction)": 3.0, + "Concentration factor": 50000.0, + "Template volume [μl]": 3.6, + "Conc. [cp/mL] (undiluted sample)": 1808181441523.5188, + "Reaction Mix": "eGFP", + "Volume per well [μl]": 2.706, + "Conversion unit": "cp/mL", + "Total volume [μl]": 15.6, + "Conc. [cp/mL] (dPCR reaction)": 8345452.807031626, + "Conversion factor": 1000.0, + "Conc. [cp/μL] (undiluted sample)": 1808181441.5 + } + }, + { + "measurement identifier": "QIACUITY_DPCR_TEST_ID_12", + "measurement time": "1970-01-01T00:00:00+00:00", + "target DNA description": "eGFP", + "sample document": { + "sample identifier": "3_10000", + "sample role type": "Sample Role", + "well location identifier": "C3" + }, + "device control aggregate document": { + "device control document": [ + { + "device type": "dPCR", + "device identifier": "Qiacuity dPCR" + } + ] + }, + "total partition count": { + "value": 8281, + "unit": "#" + }, + "processed data aggregate document": { + "processed data document": [ + { + "number concentration": { + "value": 4496.9, + "unit": "#/μL" + }, + "positive partition count": { + "value": 6296, + "unit": "#" + }, + "data processing document": { + "fluorescence intensity threshold setting": { + "value": 19.45, + "unit": "(unitless)" + } + }, + "negative partition count": { + "value": 1985, + "unit": "#" + } + } + ] + }, + "custom information document": { + "CI (95%) (dPCR reaction)": 2.7, + "Concentration factor": 100000.0, + "Template volume [μl]": 3.6, + "Conc. [cp/mL] (undiluted sample)": 1948671691045.873, + "Reaction Mix": "eGFP", + "Volume per well [μl]": 2.703, + "Conversion unit": "cp/mL", + "Total volume [μl]": 15.6, + "Conc. [cp/mL] (dPCR reaction)": 4496934.671644322, + "Conversion factor": 1000.0, + "Conc. [cp/μL] (undiluted sample)": 1948671691.0 + } + }, + { + "measurement identifier": "QIACUITY_DPCR_TEST_ID_13", + "measurement time": "1970-01-01T00:00:00+00:00", + "target DNA description": "eGFP", + "sample document": { + "sample identifier": "3_10000", + "sample role type": "Sample Role", + "well location identifier": "C4" + }, + "device control aggregate document": { + "device control document": [ + { + "device type": "dPCR", + "device identifier": "Qiacuity dPCR" + } + ] + }, + "total partition count": { + "value": 8280, + "unit": "#" + }, + "processed data aggregate document": { + "processed data document": [ + { + "number concentration": { + "value": 4286.8, + "unit": "#/μL" + }, + "positive partition count": { + "value": 6114, + "unit": "#" + }, + "data processing document": { + "fluorescence intensity threshold setting": { + "value": 19.45, + "unit": "(unitless)" + } + }, + "negative partition count": { + "value": 2166, + "unit": "#" + } + } + ] + }, + "custom information document": { + "CI (95%) (dPCR reaction)": 2.7, + "Concentration factor": 100000.0, + "Template volume [μl]": 3.6, + "Conc. [cp/mL] (undiluted sample)": 1857632049793.4663, + "Reaction Mix": "eGFP", + "Volume per well [μl]": 2.662, + "Conversion unit": "cp/mL", + "Total volume [μl]": 15.6, + "Conc. [cp/mL] (dPCR reaction)": 4286843.191831076, + "Conversion factor": 1000.0, + "Conc. [cp/μL] (undiluted sample)": 1857632049.8 + } + }, + { + "measurement identifier": "QIACUITY_DPCR_TEST_ID_14", + "measurement time": "1970-01-01T00:00:00+00:00", + "target DNA description": "eGFP", + "sample document": { + "sample identifier": "GFP+_10000", + "sample role type": "Sample Role", + "well location identifier": "C5" + }, + "device control aggregate document": { + "device control document": [ + { + "device type": "dPCR", + "device identifier": "Qiacuity dPCR" + } + ] + }, + "total partition count": { + "value": 8257, + "unit": "#" + }, + "processed data aggregate document": { + "processed data document": [ + { + "number concentration": { + "value": 4460.6, + "unit": "#/μL" + }, + "positive partition count": { + "value": 6193, + "unit": "#" + }, + "data processing document": { + "fluorescence intensity threshold setting": { + "value": 19.45, + "unit": "(unitless)" + } + }, + "negative partition count": { + "value": 2064, + "unit": "#" + } + } + ] + }, + "custom information document": { + "CI (95%) (dPCR reaction)": 2.7, + "Concentration factor": 10000.0, + "Template volume [μl]": 3.6, + "Conc. [cp/mL] (undiluted sample)": 193294447776.66205, + "Reaction Mix": "eGFP", + "Volume per well [μl]": 2.645, + "Conversion unit": "cp/mL", + "Total volume [μl]": 15.6, + "Conc. [cp/mL] (dPCR reaction)": 4460641.102538355, + "Conversion factor": 1000.0, + "Conc. [cp/μL] (undiluted sample)": 193294447.8 + } + }, + { + "measurement identifier": "QIACUITY_DPCR_TEST_ID_15", + "measurement time": "1970-01-01T00:00:00+00:00", + "target DNA description": "eGFP", + "sample document": { + "sample identifier": "4_5000", + "sample role type": "Sample Role", + "well location identifier": "D1" + }, + "device control aggregate document": { + "device control document": [ + { + "device type": "dPCR", + "device identifier": "Qiacuity dPCR" + } + ] + }, + "total partition count": { + "value": 8285, + "unit": "#" + }, + "processed data aggregate document": { + "processed data document": [ + { + "number concentration": { + "value": 7045.4, + "unit": "#/μL" + }, + "positive partition count": { + "value": 7476, + "unit": "#" + }, + "data processing document": { + "fluorescence intensity threshold setting": { + "value": 19.45, + "unit": "(unitless)" + } + }, + "negative partition count": { + "value": 809, + "unit": "#" + } + } + ] + }, + "custom information document": { + "CI (95%) (dPCR reaction)": 2.8, + "Concentration factor": 50000.0, + "Template volume [μl]": 3.6, + "Conc. [cp/mL] (undiluted sample)": 1526512249740.832, + "Reaction Mix": "eGFP", + "Volume per well [μl]": 2.81, + "Conversion unit": "cp/mL", + "Total volume [μl]": 15.6, + "Conc. [cp/mL] (dPCR reaction)": 7045441.152649994, + "Conversion factor": 1000.0, + "Conc. [cp/μL] (undiluted sample)": 1526512249.7 + } + }, + { + "measurement identifier": "QIACUITY_DPCR_TEST_ID_16", + "measurement time": "1970-01-01T00:00:00+00:00", + "target DNA description": "eGFP", + "sample document": { + "sample identifier": "4_5000", + "sample role type": "Sample Role", + "well location identifier": "D2" + }, + "device control aggregate document": { + "device control document": [ + { + "device type": "dPCR", + "device identifier": "Qiacuity dPCR" + } + ] + }, + "total partition count": { + "value": 8278, + "unit": "#" + }, + "processed data aggregate document": { + "processed data document": [ + { + "number concentration": { + "value": 7069.1, + "unit": "#/μL" + }, + "positive partition count": { + "value": 7418, + "unit": "#" + }, + "data processing document": { + "fluorescence intensity threshold setting": { + "value": 19.45, + "unit": "(unitless)" + } + }, + "negative partition count": { + "value": 860, + "unit": "#" + } + } + ] + }, + "custom information document": { + "CI (95%) (dPCR reaction)": 2.8, + "Concentration factor": 50000.0, + "Template volume [μl]": 3.6, + "Conc. [cp/mL] (undiluted sample)": 1531629117357.8635, + "Reaction Mix": "eGFP", + "Volume per well [μl]": 2.726, + "Conversion unit": "cp/mL", + "Total volume [μl]": 15.6, + "Conc. [cp/mL] (dPCR reaction)": 7069057.464728602, + "Conversion factor": 1000.0, + "Conc. [cp/μL] (undiluted sample)": 1531629117.4 + } + }, + { + "measurement identifier": "QIACUITY_DPCR_TEST_ID_17", + "measurement time": "1970-01-01T00:00:00+00:00", + "target DNA description": "eGFP", + "sample document": { + "sample identifier": "4_10000", + "sample role type": "Sample Role", + "well location identifier": "D3" + }, + "device control aggregate document": { + "device control document": [ + { + "device type": "dPCR", + "device identifier": "Qiacuity dPCR" + } + ] + }, + "total partition count": { + "value": 8257, + "unit": "#" + }, + "processed data aggregate document": { + "processed data document": [ + { + "number concentration": { + "value": 3853.2, + "unit": "#/μL" + }, + "positive partition count": { + "value": 5821, + "unit": "#" + }, + "data processing document": { + "fluorescence intensity threshold setting": { + "value": 19.45, + "unit": "(unitless)" + } + }, + "negative partition count": { + "value": 2436, + "unit": "#" + } + } + ] + }, + "custom information document": { + "CI (95%) (dPCR reaction)": 2.7, + "Concentration factor": 100000.0, + "Template volume [μl]": 3.6, + "Conc. [cp/mL] (undiluted sample)": 1669714154540.81, + "Reaction Mix": "eGFP", + "Volume per well [μl]": 2.696, + "Conversion unit": "cp/mL", + "Total volume [μl]": 15.6, + "Conc. [cp/mL] (dPCR reaction)": 3853186.510478793, + "Conversion factor": 1000.0, + "Conc. [cp/μL] (undiluted sample)": 1669714154.5 + } + }, + { + "measurement identifier": "QIACUITY_DPCR_TEST_ID_18", + "measurement time": "1970-01-01T00:00:00+00:00", + "target DNA description": "eGFP", + "sample document": { + "sample identifier": "4_10000", + "sample role type": "Sample Role", + "well location identifier": "D4" + }, + "device control aggregate document": { + "device control document": [ + { + "device type": "dPCR", + "device identifier": "Qiacuity dPCR" + } + ] + }, + "total partition count": { + "value": 8276, + "unit": "#" + }, + "processed data aggregate document": { + "processed data document": [ + { + "number concentration": { + "value": 3657.5, + "unit": "#/μL" + }, + "positive partition count": { + "value": 5640, + "unit": "#" + }, + "data processing document": { + "fluorescence intensity threshold setting": { + "value": 19.45, + "unit": "(unitless)" + } + }, + "negative partition count": { + "value": 2636, + "unit": "#" + } + } + ] + }, + "custom information document": { + "CI (95%) (dPCR reaction)": 2.8, + "Concentration factor": 100000.0, + "Template volume [μl]": 3.6, + "Conc. [cp/mL] (undiluted sample)": 1584916941610.0308, + "Reaction Mix": "eGFP", + "Volume per well [μl]": 2.662, + "Conversion unit": "cp/mL", + "Total volume [μl]": 15.6, + "Conc. [cp/mL] (dPCR reaction)": 3657500.634484687, + "Conversion factor": 1000.0, + "Conc. [cp/μL] (undiluted sample)": 1584916941.6 + } + }, + { + "measurement identifier": "QIACUITY_DPCR_TEST_ID_19", + "measurement time": "1970-01-01T00:00:00+00:00", + "target DNA description": "eGFP", + "sample document": { + "sample identifier": "GFP+_10000", + "sample role type": "Sample Role", + "well location identifier": "D5" + }, + "device control aggregate document": { + "device control document": [ + { + "device type": "dPCR", + "device identifier": "Qiacuity dPCR" + } + ] + }, + "total partition count": { + "value": 8269, + "unit": "#" + }, + "processed data aggregate document": { + "processed data document": [ + { + "number concentration": { + "value": 4623.5, + "unit": "#/μL" + }, + "positive partition count": { + "value": 6251, + "unit": "#" + }, + "data processing document": { + "fluorescence intensity threshold setting": { + "value": 19.45, + "unit": "(unitless)" + } + }, + "negative partition count": { + "value": 2018, + "unit": "#" + } + } + ] + }, + "custom information document": { + "CI (95%) (dPCR reaction)": 2.7, + "Concentration factor": 10000.0, + "Template volume [μl]": 3.6, + "Conc. [cp/mL] (undiluted sample)": 200350910227.60248, + "Reaction Mix": "eGFP", + "Volume per well [μl]": 2.596, + "Conversion unit": "cp/mL", + "Total volume [μl]": 15.6, + "Conc. [cp/mL] (dPCR reaction)": 4623482.543713903, + "Conversion factor": 1000.0, + "Conc. [cp/μL] (undiluted sample)": 200350910.2 + } + }, + { + "measurement identifier": "QIACUITY_DPCR_TEST_ID_20", + "measurement time": "1970-01-01T00:00:00+00:00", + "target DNA description": "eGFP", + "sample document": { + "sample identifier": "5_5000", + "sample role type": "Sample Role", + "well location identifier": "E1" + }, + "device control aggregate document": { + "device control document": [ + { + "device type": "dPCR", + "device identifier": "Qiacuity dPCR" + } + ] + }, + "total partition count": { + "value": 8274, + "unit": "#" + }, + "processed data aggregate document": { + "processed data document": [ + { + "number concentration": { + "value": 6629.6, + "unit": "#/μL" + }, + "positive partition count": { + "value": 7345, + "unit": "#" + }, + "data processing document": { + "fluorescence intensity threshold setting": { + "value": 19.45, + "unit": "(unitless)" + } + }, + "negative partition count": { + "value": 929, + "unit": "#" + } + } + ] + }, + "custom information document": { + "CI (95%) (dPCR reaction)": 2.8, + "Concentration factor": 50000.0, + "Template volume [μl]": 3.6, + "Conc. [cp/mL] (undiluted sample)": 1436419478719.8535, + "Reaction Mix": "eGFP", + "Volume per well [μl]": 2.807, + "Conversion unit": "cp/mL", + "Total volume [μl]": 15.6, + "Conc. [cp/mL] (dPCR reaction)": 6629628.363322401, + "Conversion factor": 1000.0, + "Conc. [cp/μL] (undiluted sample)": 1436419478.7 + } + }, + { + "measurement identifier": "QIACUITY_DPCR_TEST_ID_21", + "measurement time": "1970-01-01T00:00:00+00:00", + "target DNA description": "eGFP", + "sample document": { + "sample identifier": "5_5000", + "sample role type": "Sample Role", + "well location identifier": "E2" + }, + "device control aggregate document": { + "device control document": [ + { + "device type": "dPCR", + "device identifier": "Qiacuity dPCR" + } + ] + }, + "total partition count": { + "value": 8275, + "unit": "#" + }, + "processed data aggregate document": { + "processed data document": [ + { + "number concentration": { + "value": 6718.0, + "unit": "#/μL" + }, + "positive partition count": { + "value": 7313, + "unit": "#" + }, + "data processing document": { + "fluorescence intensity threshold setting": { + "value": 19.45, + "unit": "(unitless)" + } + }, + "negative partition count": { + "value": 962, + "unit": "#" + } + } + ] + }, + "custom information document": { + "CI (95%) (dPCR reaction)": 2.8, + "Concentration factor": 50000.0, + "Template volume [μl]": 3.6, + "Conc. [cp/mL] (undiluted sample)": 1455572998874.9473, + "Reaction Mix": "eGFP", + "Volume per well [μl]": 2.726, + "Conversion unit": "cp/mL", + "Total volume [μl]": 15.6, + "Conc. [cp/mL] (dPCR reaction)": 6718029.225576681, + "Conversion factor": 1000.0, + "Conc. [cp/μL] (undiluted sample)": 1455572998.9 + } + }, + { + "measurement identifier": "QIACUITY_DPCR_TEST_ID_22", + "measurement time": "1970-01-01T00:00:00+00:00", + "target DNA description": "eGFP", + "sample document": { + "sample identifier": "5_10000", + "sample role type": "Sample Role", + "well location identifier": "E3" + }, + "device control aggregate document": { + "device control document": [ + { + "device type": "dPCR", + "device identifier": "Qiacuity dPCR" + } + ] + }, + "total partition count": { + "value": 8263, + "unit": "#" + }, + "processed data aggregate document": { + "processed data document": [ + { + "number concentration": { + "value": 3537.6, + "unit": "#/μL" + }, + "positive partition count": { + "value": 5510, + "unit": "#" + }, + "data processing document": { + "fluorescence intensity threshold setting": { + "value": 19.45, + "unit": "(unitless)" + } + }, + "negative partition count": { + "value": 2753, + "unit": "#" + } + } + ] + }, + "custom information document": { + "CI (95%) (dPCR reaction)": 2.8, + "Concentration factor": 100000.0, + "Template volume [μl]": 3.6, + "Conc. [cp/mL] (undiluted sample)": 1532943076917.869, + "Reaction Mix": "eGFP", + "Volume per well [μl]": 2.644, + "Conversion unit": "cp/mL", + "Total volume [μl]": 15.6, + "Conc. [cp/mL] (dPCR reaction)": 3537560.946733544, + "Conversion factor": 1000.0, + "Conc. [cp/μL] (undiluted sample)": 1532943076.9 + } + }, + { + "measurement identifier": "QIACUITY_DPCR_TEST_ID_23", + "measurement time": "1970-01-01T00:00:00+00:00", + "target DNA description": "eGFP", + "sample document": { + "sample identifier": "5_10000", + "sample role type": "Sample Role", + "well location identifier": "E4" + }, + "device control aggregate document": { + "device control document": [ + { + "device type": "dPCR", + "device identifier": "Qiacuity dPCR" + } + ] + }, + "total partition count": { + "value": 8264, + "unit": "#" + }, + "processed data aggregate document": { + "processed data document": [ + { + "number concentration": { + "value": 3386.7, + "unit": "#/μL" + }, + "positive partition count": { + "value": 5398, + "unit": "#" + }, + "data processing document": { + "fluorescence intensity threshold setting": { + "value": 19.45, + "unit": "(unitless)" + } + }, + "negative partition count": { + "value": 2866, + "unit": "#" + } + } + ] + }, + "custom information document": { + "CI (95%) (dPCR reaction)": 2.8, + "Concentration factor": 100000.0, + "Template volume [μl]": 3.6, + "Conc. [cp/mL] (undiluted sample)": 1467571324674.5393, + "Reaction Mix": "eGFP", + "Volume per well [μl]": 2.661, + "Conversion unit": "cp/mL", + "Total volume [μl]": 15.6, + "Conc. [cp/mL] (dPCR reaction)": 3386703.056941245, + "Conversion factor": 1000.0, + "Conc. [cp/μL] (undiluted sample)": 1467571324.7 + } + }, + { + "measurement identifier": "QIACUITY_DPCR_TEST_ID_24", + "measurement time": "1970-01-01T00:00:00+00:00", + "target DNA description": "eGFP", + "sample document": { + "sample identifier": "NTC_1", + "sample role type": "Sample Role", + "well location identifier": "E5" + }, + "device control aggregate document": { + "device control document": [ + { + "device type": "dPCR", + "device identifier": "Qiacuity dPCR" + } + ] + }, + "total partition count": { + "value": 8263, + "unit": "#" + }, + "processed data aggregate document": { + "processed data document": [ + { + "number concentration": { + "value": 0.0, + "unit": "#/μL" + }, + "positive partition count": { + "value": 0, + "unit": "#" + }, + "data processing document": { + "fluorescence intensity threshold setting": { + "value": 19.45, + "unit": "(unitless)" + } + }, + "negative partition count": { + "value": 8263, + "unit": "#" + } + } + ] + }, + "custom information document": { + "CI (95%) (dPCR reaction)": "-", + "Concentration factor": 1.0, + "Template volume [μl]": 3.6, + "Conc. [cp/mL] (undiluted sample)": 0.0, + "Reaction Mix": "eGFP", + "Volume per well [μl]": 2.621, + "Conversion unit": "cp/mL", + "Total volume [μl]": 15.6, + "Conc. [cp/mL] (dPCR reaction)": 0.0, + "Conversion factor": 1000.0, + "Conc. [cp/μL] (undiluted sample)": 0.0 + } + }, + { + "measurement identifier": "QIACUITY_DPCR_TEST_ID_25", + "measurement time": "1970-01-01T00:00:00+00:00", + "target DNA description": "eGFP", + "sample document": { + "sample identifier": "6_5000", + "sample role type": "Sample Role", + "well location identifier": "F1" + }, + "device control aggregate document": { + "device control document": [ + { + "device type": "dPCR", + "device identifier": "Qiacuity dPCR" + } + ] + }, + "total partition count": { + "value": 8259, + "unit": "#" + }, + "processed data aggregate document": { + "processed data document": [ + { + "number concentration": { + "value": 6685.3, + "unit": "#/μL" + }, + "positive partition count": { + "value": 7350, + "unit": "#" + }, + "data processing document": { + "fluorescence intensity threshold setting": { + "value": 19.45, + "unit": "(unitless)" + } + }, + "negative partition count": { + "value": 909, + "unit": "#" + } + } + ] + }, + "custom information document": { + "CI (95%) (dPCR reaction)": 2.8, + "Concentration factor": 50000.0, + "Template volume [μl]": 3.6, + "Conc. [cp/mL] (undiluted sample)": 1448491376471.0164, + "Reaction Mix": "eGFP", + "Volume per well [μl]": 2.809, + "Conversion unit": "cp/mL", + "Total volume [μl]": 15.6, + "Conc. [cp/mL] (dPCR reaction)": 6685344.814481614, + "Conversion factor": 1000.0, + "Conc. [cp/μL] (undiluted sample)": 1448491376.5 + } + }, + { + "measurement identifier": "QIACUITY_DPCR_TEST_ID_26", + "measurement time": "1970-01-01T00:00:00+00:00", + "target DNA description": "eGFP", + "sample document": { + "sample identifier": "6_5000", + "sample role type": "Sample Role", + "well location identifier": "F2" + }, + "device control aggregate document": { + "device control document": [ + { + "device type": "dPCR", + "device identifier": "Qiacuity dPCR" + } + ] + }, + "total partition count": { + "value": 8247, + "unit": "#" + }, + "processed data aggregate document": { + "processed data document": [ + { + "number concentration": { + "value": 6638.5, + "unit": "#/μL" + }, + "positive partition count": { + "value": 7287, + "unit": "#" + }, + "data processing document": { + "fluorescence intensity threshold setting": { + "value": 19.45, + "unit": "(unitless)" + } + }, + "negative partition count": { + "value": 960, + "unit": "#" + } + } + ] + }, + "custom information document": { + "CI (95%) (dPCR reaction)": 2.8, + "Concentration factor": 50000.0, + "Template volume [μl]": 3.6, + "Conc. [cp/mL] (undiluted sample)": 1438331442395.4546, + "Reaction Mix": "eGFP", + "Volume per well [μl]": 2.757, + "Conversion unit": "cp/mL", + "Total volume [μl]": 15.6, + "Conc. [cp/mL] (dPCR reaction)": 6638452.811055944, + "Conversion factor": 1000.0, + "Conc. [cp/μL] (undiluted sample)": 1438331442.4 + } + }, + { + "measurement identifier": "QIACUITY_DPCR_TEST_ID_27", + "measurement time": "1970-01-01T00:00:00+00:00", + "target DNA description": "eGFP", + "sample document": { + "sample identifier": "6_10000", + "sample role type": "Sample Role", + "well location identifier": "F3" + }, + "device control aggregate document": { + "device control document": [ + { + "device type": "dPCR", + "device identifier": "Qiacuity dPCR" + } + ] + }, + "total partition count": { + "value": 8249, + "unit": "#" + }, + "processed data aggregate document": { + "processed data document": [ + { + "number concentration": { + "value": 3545.9, + "unit": "#/μL" + }, + "positive partition count": { + "value": 5601, + "unit": "#" + }, + "data processing document": { + "fluorescence intensity threshold setting": { + "value": 19.45, + "unit": "(unitless)" + } + }, + "negative partition count": { + "value": 2648, + "unit": "#" + } + } + ] + }, + "custom information document": { + "CI (95%) (dPCR reaction)": 2.8, + "Concentration factor": 100000.0, + "Template volume [μl]": 3.6, + "Conc. [cp/mL] (undiluted sample)": 1536578275692.3123, + "Reaction Mix": "eGFP", + "Volume per well [μl]": 2.727, + "Conversion unit": "cp/mL", + "Total volume [μl]": 15.6, + "Conc. [cp/mL] (dPCR reaction)": 3545949.866982259, + "Conversion factor": 1000.0, + "Conc. [cp/μL] (undiluted sample)": 1536578275.7 + } + }, + { + "measurement identifier": "QIACUITY_DPCR_TEST_ID_28", + "measurement time": "1970-01-01T00:00:00+00:00", + "target DNA description": "eGFP", + "sample document": { + "sample identifier": "6_10000", + "sample role type": "Sample Role", + "well location identifier": "F4" + }, + "device control aggregate document": { + "device control document": [ + { + "device type": "dPCR", + "device identifier": "Qiacuity dPCR" + } + ] + }, + "total partition count": { + "value": 8212, + "unit": "#" + }, + "processed data aggregate document": { + "processed data document": [ + { + "number concentration": { + "value": 3456.3, + "unit": "#/μL" + }, + "positive partition count": { + "value": 5382, + "unit": "#" + }, + "data processing document": { + "fluorescence intensity threshold setting": { + "value": 19.45, + "unit": "(unitless)" + } + }, + "negative partition count": { + "value": 2830, + "unit": "#" + } + } + ] + }, + "custom information document": { + "CI (95%) (dPCR reaction)": 2.8, + "Concentration factor": 100000.0, + "Template volume [μl]": 3.6, + "Conc. [cp/mL] (undiluted sample)": 1497729420400.2473, + "Reaction Mix": "eGFP", + "Volume per well [μl]": 2.623, + "Conversion unit": "cp/mL", + "Total volume [μl]": 15.6, + "Conc. [cp/mL] (dPCR reaction)": 3456298.662462109, + "Conversion factor": 1000.0, + "Conc. [cp/μL] (undiluted sample)": 1497729420.4 + } + }, + { + "measurement identifier": "QIACUITY_DPCR_TEST_ID_29", + "measurement time": "1970-01-01T00:00:00+00:00", + "target DNA description": "eGFP", + "sample document": { + "sample identifier": "NTC_1", + "sample role type": "Sample Role", + "well location identifier": "F5" + }, + "device control aggregate document": { + "device control document": [ + { + "device type": "dPCR", + "device identifier": "Qiacuity dPCR" + } + ] + }, + "total partition count": { + "value": 6287, + "unit": "#" + }, + "processed data aggregate document": { + "processed data document": [ + { + "number concentration": { + "value": 0.0, + "unit": "#/μL" + }, + "positive partition count": { + "value": 0, + "unit": "#" + }, + "data processing document": { + "fluorescence intensity threshold setting": { + "value": 19.45, + "unit": "(unitless)" + } + }, + "negative partition count": { + "value": 6287, + "unit": "#" + } + } + ] + }, + "custom information document": { + "CI (95%) (dPCR reaction)": "-", + "Concentration factor": 1.0, + "Template volume [μl]": 3.6, + "Conc. [cp/mL] (undiluted sample)": 0.0, + "Reaction Mix": "eGFP", + "Volume per well [μl]": 2.639, + "Conversion unit": "cp/mL", + "Total volume [μl]": 15.6, + "Conc. [cp/mL] (dPCR reaction)": 0.0, + "Conversion factor": 1000.0, + "Conc. [cp/μL] (undiluted sample)": 0.0 + } + }, + { + "measurement identifier": "QIACUITY_DPCR_TEST_ID_30", + "measurement time": "1970-01-01T00:00:00+00:00", + "target DNA description": "eGFP", + "sample document": { + "sample identifier": "NTC_1", + "sample role type": "Sample Role", + "well location identifier": "G5" + }, + "device control aggregate document": { + "device control document": [ + { + "device type": "dPCR", + "device identifier": "Qiacuity dPCR" + } + ] + }, + "total partition count": { + "value": 8249, + "unit": "#" + }, + "processed data aggregate document": { + "processed data document": [ + { + "number concentration": { + "value": 0.386, + "unit": "#/μL" + }, + "positive partition count": { + "value": 1, + "unit": "#" + }, + "data processing document": { + "fluorescence intensity threshold setting": { + "value": 19.45, + "unit": "(unitless)" + } + }, + "negative partition count": { + "value": 8248, + "unit": "#" + } + } + ] + }, + "custom information document": { + "CI (95%) (dPCR reaction)": 147.5, + "Concentration factor": 1.0, + "Template volume [μl]": 3.6, + "Conc. [cp/mL] (undiluted sample)": 1670.669600924, + "Reaction Mix": "eGFP", + "Volume per well [μl]": 2.676, + "Conversion unit": "cp/mL", + "Total volume [μl]": 15.6, + "Conc. [cp/mL] (dPCR reaction)": 385.539138675, + "Conversion factor": 1000.0, + "Conc. [cp/μL] (undiluted sample)": 1.671 + } + }, + { + "measurement identifier": "QIACUITY_DPCR_TEST_ID_31", + "measurement time": "1970-01-01T00:00:00+00:00", + "target DNA description": "eGFP", + "sample document": { + "sample identifier": "NTC_1", + "sample role type": "Sample Role", + "well location identifier": "H5" + }, + "device control aggregate document": { + "device control document": [ + { + "device type": "dPCR", + "device identifier": "Qiacuity dPCR" + } + ] + }, + "total partition count": { + "value": 8211, + "unit": "#" + }, + "processed data aggregate document": { + "processed data document": [ + { + "number concentration": { + "value": 0.0, + "unit": "#/μL" + }, + "positive partition count": { + "value": 0, + "unit": "#" + }, + "data processing document": { + "fluorescence intensity threshold setting": { + "value": 19.45, + "unit": "(unitless)" + } + }, + "negative partition count": { + "value": 8211, + "unit": "#" + } + } + ] + }, + "custom information document": { + "CI (95%) (dPCR reaction)": "-", + "Concentration factor": 1.0, + "Template volume [μl]": 3.6, + "Conc. [cp/mL] (undiluted sample)": 0.0, + "Reaction Mix": "eGFP", + "Volume per well [μl]": 2.734, + "Conversion unit": "cp/mL", + "Total volume [μl]": 15.6, + "Conc. [cp/mL] (dPCR reaction)": 0.0, + "Conversion factor": 1000.0, + "Conc. [cp/μL] (undiluted sample)": 0.0 + } + } + ] + } + } + ], + "data system document": { + "file name": "qiacuity_dpcr_example04.csv", + "UNC path": "tests/parsers/qiacuity_dpcr/testdata/qiacuity_dpcr_example04.csv", + "software name": "Qiacuity Software Suite", + "ASM converter name": "allotropy_qiacuity_dpcr", + "ASM converter version": "0.1.111" + } + } +}