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## Generating Physlr Physical Map with stLFR reads
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To construct a physical map, you need linked reads from 10X Genomics or stLFR. In addition, to visualize the correctness and contiguity of the physical map, you need a reference genome.
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In this example, the linked reads and reference genome are called `linkedreads.fq.gz` and `reference.fa`, respectively. The linked reads are from stLFR so we specify `minimizer_overlap=stLFR` to use the default value for stLFR reads.
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In this example, the linked reads and reference genome are called `linkedreads.fq.gz` and `reference.fa`, respectively. The linked reads are from stLFR so we specify `protocol=stlfr` to use the default value for stLFR reads.
## Scaffolding a draft assembly with Physlr Physical Map
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To scaffold a draft assembly, you need linked reads from 10X Genomics or stLFR, and an existing assembly. In addition, to calculate Quast summary metrics for the Physlr scaffolded assembly, you need a reference genome.
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In this example, the linked reads, draft assembly, and reference genome are called `linkedreads.fq.gz`, `draft.fa`, `reference.fa`, respectively. The linked reads are from 10X Genomics so we specify `minimizer_overlap=10X` to use the default value for 10X Genomics reads.
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In this example, the linked reads, draft assembly, and reference genome are called `linkedreads.fq.gz`, `draft.fa`, `reference.fa`, respectively. The linked reads are from 10X Genomics so we specify `protocol=10x` to use the default value for 10X Genomics reads.
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