diff --git a/_template.Rmd b/_template.Rmd index b684384..866a457 100644 --- a/_template.Rmd +++ b/_template.Rmd @@ -127,6 +127,33 @@ We will perform a couple of quality control steps to deal with negative values i ```{r} #| label: "quality-control" +krsa_filter_lowPeps_cycles <- function(raw_data, threshold, min_pct_cycles = 0.8, + samples = NULL, groups = NULL) { + raw_data %>% + { + if (!is.null(samples)) dplyr::filter(., SampleName %in% samples) else . + } %>% + { + if (!is.null(groups)) dplyr::filter(., Group %in% groups) else . + } %>% + dplyr::filter(ExposureTime == max(ExposureTime)) %>% + dplyr::select(SampleName, Peptide, Cycle, Signal) %>% + dplyr::group_by(Peptide, SampleName) %>% + dplyr::summarise(pct_present = mean(Signal >= threshold, na.rm = TRUE), .groups = "drop") %>% + dplyr::group_by(Peptide) %>% + dplyr::filter(all(pct_present >= min_pct_cycles)) %>% + dplyr::pull(Peptide) %>% + unique() -> p + + message(paste( + "Filtered out", length(unique(raw_data$Peptide)) - length(p), + "Peptides not present in at least", paste0(round(min_pct_cycles * 100), "%"), + "of cycles in every sample" + )) + + p +} + # qc processing qc_data <- krsa_qc_steps(raw_data) |> @@ -137,11 +164,23 @@ qc_data <- krsa_qc_steps(raw_data) |> groups <- unique(qc_data[["Group"]]) +# ---- NEW: filter out peptides not consistently present across cycles ---- +cycle_peptides <- krsa_filter_lowPeps_cycles( + raw_data = qc_data, + threshold = 5L, # same as used later for low-signal filter + min_pct_cycles = 0.8 # keep peptides present in ≥80% of cycles +) + +# Keep only those peptides for all downstream steps +qc_data_filtered <- qc_data |> + dplyr::filter(Peptide %in% cycle_peptides) + # extract end level signal values @ all max exposure time (100ms) -data_pw_max <- krsa_extractEndPointMaxExp(qc_data, chipType) +data_pw_max <- krsa_extractEndPointMaxExp(qc_data_filtered, chipType) # extract end level signal values end level @ all exposure times -data_pw <- krsa_extractEndPoint(qc_data, chipType) +data_pw <- krsa_extractEndPoint(qc_data_filtered, chipType) + ``` ## QC Steps and Model Fitting diff --git a/kinome_data/_KRSAsOutput_Median_SigmBg_260716141146.txt b/kinome_data/_KRSAsOutput_Median_SigmBg_260716141146.txt new file mode 100644 index 0000000..3b507de --- /dev/null +++ b/kinome_data/_KRSAsOutput_Median_SigmBg_260716141146.txt @@ -0,0 +1,177 @@ +BioNavigator Version 6.3.67.0 PamGrid Version QuantitationType Median_SigmBg Protocol file name preprocessingDate Thu, 16 Jul 2026 14:11:46 GMT + Barcode 720061316 720061316 720061316 720061316 720061316 720061316 720061316 720061316 720061316 720061316 720061316 720061316 720061316 720061316 720061316 720061316 720061316 720061316 720061316 720061316 720061316 720061316 720061316 720061316 720061316 720061316 720061316 720061316 720061316 720061316 720061316 720061316 720061316 720061316 720061316 720061316 720061316 720061316 720061316 720061316 720061316 720061316 720061316 720061316 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NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA + Lamp refrence power NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA + PamChip Location 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 + Array A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 + Visual QC OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK + Operator MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM + Article number 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 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87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 + Sample name M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI + Test Condition NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA + Supergroup NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA + GridID 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 3 4 4 4 4 4 4 4 4 5 6 6 6 6 6 6 6 6 7 7 7 7 7 7 7 7 8 8 8 8 8 8 8 8 9 10 10 10 10 10 10 10 10 11 12 12 12 12 12 12 12 12 13 13 13 13 13 13 13 13 14 14 14 14 14 14 14 14 15 16 16 16 16 16 16 16 16 17 18 18 18 18 18 18 18 18 19 19 19 19 19 19 19 19 20 20 20 20 20 20 20 20 21 22 22 22 22 22 22 22 22 23 24 24 24 24 24 24 24 24 25 25 25 25 25 25 25 25 26 26 26 26 26 26 26 26 27 28 28 28 28 28 28 28 28 29 30 30 30 30 30 30 30 30 31 31 31 31 31 31 31 31 32 32 32 32 32 32 32 32 33 34 34 34 34 34 34 34 34 35 36 36 36 36 36 36 36 36 37 37 37 37 37 37 37 37 38 38 38 38 38 38 38 38 39 40 40 40 40 40 40 40 40 41 42 42 42 42 42 42 42 42 43 43 43 43 43 43 43 43 44 44 44 44 44 44 44 44 45 46 46 46 46 46 46 46 46 47 48 48 48 48 48 48 48 48 49 49 49 49 49 49 49 49 50 50 50 50 50 50 50 50 51 52 52 52 52 52 52 52 52 53 54 54 54 54 54 54 54 54 55 55 55 55 55 55 55 55 56 56 56 56 56 56 56 56 57 58 58 58 58 58 58 58 58 59 60 60 60 60 60 60 60 60 61 61 61 61 61 61 61 61 62 62 62 62 62 62 62 62 63 64 64 64 64 64 64 64 64 65 66 66 66 66 66 66 66 66 67 67 67 67 67 67 67 67 68 68 68 68 68 68 68 68 69 70 70 70 70 70 70 70 70 71 72 72 72 72 72 72 72 72 + Grid type 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 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230.0 241.0 476.0 1234.0 1204.0 1194.0 1183.0 1175.0 1183.0 1167.0 1203.0 2409.0 0.0 22.0 66.0 54.0 13.0 20.0 0.0 2933.0 44.0 47.0 41.0 42.0 43.0 40.0 45.0 41.0 230.0 230.0 231.0 224.0 225.0 225.0 227.0 212.0 427.0 1085.0 1084.0 1077.0 1094.0 1096.0 1062.0 1073.0 1045.0 2160.0 182.0 233.0 185.0 194.0 153.0 129.0 101.0 3405.0 38.0 38.0 43.0 40.0 40.0 41.0 43.0 41.0 214.0 209.0 219.0 211.0 211.0 209.0 200.0 216.0 423.0 1098.0 1067.0 1063.0 1046.0 1044.0 1040.0 1029.0 1059.0 2138.0 102.0 99.0 77.0 75.0 55.0 30.0 6.0 3030.0 34.0 38.0 33.0 36.0 35.0 37.0 36.0 39.0 192.0 185.0 190.0 185.0 180.0 184.0 177.0 185.0 371.0 967.0 955.0 950.0 953.0 937.0 942.0 921.0 954.0 1874.0 222.0 266.0 245.0 230.0 220.0 218.0 180.0 3511.0 39.0 38.0 37.0 35.0 33.0 36.0 33.0 35.0 189.0 186.0 179.0 179.0 175.0 176.0 166.0 179.0 366.0 929.0 922.0 920.0 905.0 907.0 900.0 877.0 902.0 1851.0 100.0 126.0 118.0 115.0 79.0 73.0 55.0 3045.0 45.0 38.0 41.0 38.0 41.0 40.0 38.0 39.0 222.0 217.0 213.0 215.0 218.0 205.0 201.0 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1 1 1 1 1 1 1 1 + Lamp power NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA 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2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 + Array A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A2 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A3 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 A4 + Visual QC OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK + Operator MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM MM + Article number 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 87202 + Sample name M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI M-Mouse_HI F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI F-Mouse_HI + Test Condition NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA + Supergroup NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA + GridID 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 3 4 4 4 4 4 4 4 4 5 6 6 6 6 6 6 6 6 7 7 7 7 7 7 7 7 8 8 8 8 8 8 8 8 9 10 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index 0000000..8e3c2eb --- /dev/null +++ b/krsa_suggestion.Rproj @@ -0,0 +1,13 @@ +Version: 1.0 + +RestoreWorkspace: Default +SaveWorkspace: Default +AlwaysSaveHistory: Default + +EnableCodeIndexing: Yes +UseSpacesForTab: Yes +NumSpacesForTab: 2 +Encoding: UTF-8 + +RnwWeave: Sweave +LaTeX: pdfLaTeX diff --git a/mouse.Rmd b/mouse.Rmd new file mode 100644 index 0000000..d2f94e1 --- /dev/null +++ b/mouse.Rmd @@ -0,0 +1,555 @@ +--- +title: "MouseSTK" +subtitle: "Rahaf" +date: "`r format(Sys.time(), '%d %B, %Y')`" +author: "Cognitive Disorders Research Lab" +format: + pdf: + documentclass: "scrreport" + fig-pos: 'htbp' + classoptions: ["oneside", "letterpaper"] + number-sections: true + include-in-header: + text: | + \usepackage{float} +editor: source +toc: true +params: + chip_type: STK + signal_file: "kinome_data/_KRSAsOutput_Median_SigmBg_260716141146.txt" # Change the file name to the one you have + saturation_file: "kinome_data/_KRSAsOutput_Signal_Saturation_260716141148.txt" # Change the file name to the one you have + threshold: 2 # Change the threshold if necessary + prefix: kinome + pairwise: FALSE + figure_output: NULL +--- + +```{r} +#| label: "setup" +#| include: FALSE +if (!is.null(params[["figure_output"]])) dir.create(params[["figure_output"]]) +library(KRSA) +library(knitr) +library(tidyverse) + +library(gt) # nolint: unused_import_linter. +library(furrr) # nolint: unused_import_linter. + +knitr::opts_chunk$set( # nolint: extraction_operator_linter. + collapse = TRUE, + comment = "#>", + cache = FALSE, + echo = FALSE, + message = FALSE, + warning = FALSE +) + + +# Modify the prefix if pairwise + +prefix <- params[["prefix"]] + +if (params[["pairwise"]]) { + prefix <- str_c(prefix, "p-w", sep = "_") +} + +``` + +# Introduction + +## Background + +The Pamstation12 instrument provides a profiling of kinase activity of cell or tissue samples. The device is loaded with either serine/threonine or tyrosine microarray chips. Each chip has 4 wells, so four samples can be loaded on a single chip, and the Pamstation12 can accommodate 3 chips per run. The microarray represents 144 (STK chip) or 196 (PTK chip) reporter peptides that can be phosphorylated by serine/threonine or tyrosine kinases. The device measures the degree of the phosphorylation in real time by detecting fluorescent labeled antibodies at different exposure times. The list of peptides present in each microarray can be viewed here: [STK chip](https://pamgene.com/wp-content/uploads/2020/09/STK-144-PamChip-87102.pdf), [PTK chip](https://pamgene.com/wp-content/uploads/2020/09/PTK-196-PamChip-86402.pdf) + +\newpage + +![PamGene Kinase Activity Platform](images/pamgene_workflow.png){fig-align="center"} + +![PamGene Platform Detection](images/pamgene_detectionFig.png){fig-align="center"} + +# Run Design + +Designing the placement of the samples on the chips and arrays is important to consider due to the variability across different chips and batches. During the run some wells are subject to fail and their data cannot be analyzed and shown below as red. + +```{r} +#| label: "design" +#| fig-width: 4 +#| fig-height: 4 + +tt <- tibble( + num = rep(c(3.5, 2.5, 1.5, 0.5), 3L), + chips = rep(c(2L, 5L, 8L), each = 4L), + colr = c(rep(1L, 12L)), + nms = rep(LETTERS[1L:4L], 3L) +) +rundes <- ggplot() + + geom_rect(aes(xmin = 1L, xmax = 3L, ymin = 0L, ymax = 4L), fill = "gray30") + + geom_rect(aes(xmin = 4L, xmax = 6L, ymin = 0L, ymax = 4L), fill = "gray30") + + geom_rect(aes(xmin = 7L, xmax = 9L, ymin = 0L, ymax = 4L), fill = "gray30") + + xlim(0L, 10L) + + theme_void() + +rundes + geom_point( + data = tt, aes(chips, num, color = factor(colr)), + size = 13L, show.legend = FALSE +) + + geom_text(data = tt, aes(chips, num, label = nms), size = 1.6) + + scale_color_manual(values = c("green3", "red3")) +``` + +# Results + +## Image Analysis + +The first step of analyzing the run is to convert the images taken by the PamStation of each array at different exposure times to numerical values. This is done by the Bionavigator software developed by PamGene. The software recognizes the grid of the array with the aid of the searching algorithm (Pamgrid) to correctly identify each spot on the array. The numbers produced by this software represent the median value of the foreground pixels minus the median value of the background pixels to produce the median signal minus background (Median_SigmBg). + +## Reading Data + +The first step will be reading the crosstab view bionavigator files (Median_SigmBg and Signal_Saturation) and defining the PamChip type (STK or PTK). The raw data is read and then transformed to be in tidy format for an easier analysis, modeling, and visualizing. + +```{r} +#| label: "read-data" + +run_prefix <- prefix + +chipType <- params[["chip_type"]] + +# Read crosstab view bionavigator files and tidy data +raw_data <- krsa_read( + signal_file = params[["signal_file"]], + signal_saturation = params[["saturation_file"]] +) +``` + +## QC Initial Steps and Groups Assignments + +We will perform a couple of quality control steps to deal with negative values in the data and adjust based on signal saturation (optional). Next, we will define a new column to represent the grouping. And then, we will extract end point signal values + +```{r} +#| label: "quality-control" +detect_cycle_changes_all_exposures <- function( + raw_data, + k = 5, + min_replicates = 2, + min_groups = 4, + sat_qc = TRUE +) { + + library(dplyr) + + qc_data <- krsa_qc_steps(raw_data, sat_qc = sat_qc) %>% + rename(Group = SampleName) %>% + mutate(SampleName = paste(Group, Barcode, sep = "_")) %>% + filter( + !grepl("^#REF", Peptide), + complete.cases(Signal, Cycle, ExposureTime) + ) + + if (!nrow(qc_data)) { + stop("No usable observations remained after KRSA QC.") + } + + signal_data <- qc_data %>% + group_by(Peptide, Group, SampleName, ExposureTime, Cycle) %>% + summarise( + Signal = median(Signal, na.rm = TRUE), + n_original_rows = n(), + .groups = "drop" + ) + + cycle_diff_data <- signal_data %>% + arrange(Peptide, Group, SampleName, ExposureTime, Cycle) %>% + group_by(Peptide, Group, SampleName, ExposureTime) %>% + mutate( + previous_cycle = lag(Cycle), + previous_signal = lag(Signal), + cycle_interval = Cycle - previous_cycle, + signal_difference = Signal - previous_signal, + cycle_rate = signal_difference / cycle_interval + ) %>% + ungroup() %>% + filter(is.na(cycle_interval) | cycle_interval > 0) + + cycle_change_data <- cycle_diff_data %>% + arrange(Peptide, Group, SampleName, ExposureTime, Cycle) %>% + group_by(Peptide, Group, SampleName, ExposureTime) %>% + mutate( + previous_cycle_rate = lag(cycle_rate), + cycle_rate_change = cycle_rate - previous_cycle_rate, + abs_cycle_rate_change = abs(cycle_rate_change), + first_cycle = lag(Cycle, 2), + middle_cycle = lag(Cycle), + final_cycle = Cycle, + direction = if_else( + cycle_rate_change > 0, + "Increase", + "Decrease" + ) + ) %>% + ungroup() %>% + filter( + !is.na(cycle_rate_change), + !is.na(first_cycle), + cycle_rate_change != 0 + ) + + if (!nrow(cycle_change_data)) { + stop("No cycle-rate changes could be calculated.") + } + + thresholds <- cycle_change_data %>% + group_by(ExposureTime, first_cycle, middle_cycle, final_cycle) %>% + summarise( + n_observations = n(), + median_change = median(abs_cycle_rate_change, na.rm = TRUE), + mad_change = mad(abs_cycle_rate_change, na.rm = TRUE), + threshold = median_change + k * mad_change, + .groups = "drop" + ) + + flagged <- cycle_change_data %>% + left_join( + thresholds, + by = c( + "ExposureTime", + "first_cycle", + "middle_cycle", + "final_cycle" + ) + ) %>% + filter( + !is.na(threshold), + abs_cycle_rate_change > threshold + ) + + group_flags <- flagged %>% + group_by( + Peptide, + Group, + ExposureTime, + first_cycle, + middle_cycle, + final_cycle, + direction + ) %>% + summarise( + n_replicates = n_distinct(SampleName), + replicates_affected = paste( + sort(unique(SampleName)), + collapse = ", " + ), + median_rate_change = median(cycle_rate_change, na.rm = TRUE), + median_abs_rate_change = median( + abs_cycle_rate_change, + na.rm = TRUE + ), + maximum_abs_rate_change = max( + abs_cycle_rate_change, + na.rm = TRUE + ), + threshold = first(threshold), + .groups = "drop" + ) %>% + filter(n_replicates >= min_replicates) + + cross_group_flags <- group_flags %>% + group_by( + Peptide, + ExposureTime, + first_cycle, + middle_cycle, + final_cycle, + direction + ) %>% + summarise( + n_groups = n_distinct(Group), + groups_affected = paste(sort(unique(Group)), collapse = ", "), + total_replicates_affected = sum(n_replicates), + maximum_abs_rate_change = max( + maximum_abs_rate_change, + na.rm = TRUE + ), + .groups = "drop" + ) %>% + filter(n_groups >= min_groups) + + peptide_summary <- cross_group_flags %>% + group_by(Peptide) %>% + summarise( + n_reproducible_events = n(), + n_groups_max = max(n_groups), + exposures_affected = paste( + sort(unique(ExposureTime)), + collapse = ", " + ), + cycle_regions = paste0( + first_cycle, + "→", + middle_cycle, + "→", + final_cycle, + " at exposure ", + ExposureTime, + " (", + direction, + ")", + collapse = "; " + ), + groups_affected = paste( + unique(groups_affected), + collapse = "; " + ), + maximum_abs_rate_change = max( + maximum_abs_rate_change, + na.rm = TRUE + ), + .groups = "drop" + ) %>% + arrange(desc(n_reproducible_events), Peptide) + + list( + removed_peptides = peptide_summary$Peptide, + peptide_summary = peptide_summary, + cross_group_flags = cross_group_flags, + group_flags = group_flags, + flagged = flagged, + thresholds = thresholds, + cycle_change_data = cycle_change_data, + cycle_diff_data = cycle_diff_data, + signal_data = signal_data, + qc_data = qc_data, + parameters = list( + k = k, + min_replicates = min_replicates, + min_groups = min_groups, + sat_qc = sat_qc + ) + ) +} + +cycle_result <- detect_cycle_changes_all_exposures( + raw_data = raw_data, + k = 5, + min_replicates = 2, + min_groups = 2, + sat_qc = TRUE +) + +qc_data_filtered <- cycle_result$qc_data %>% + filter( + !Peptide %in% cycle_result$removed_peptides + ) + + +# Extract endpoint at maximum exposure +data_pw_max <- krsa_extractEndPointMaxExp( + qc_data_filtered, + chipType +) + +# Extract endpoint at all exposures +data_pw <- krsa_extractEndPoint( + qc_data_filtered, + chipType +) + +``` + + + +## QC Steps and Model Fitting + +We will filter out peptides with low signals. In order to combine the values from different exposure times into a single value, a simple linear regression model of the *Median_SigmBg* as a function of exposure time is fitted. The slope of of the model fit and $R^2$ are then used for quality control and samples comparison. The slope is also scaled by multiplying by 100 and log2 transformed (*Slope_Transformed*). We then filter out peptides with poor linear fit and references peptides. + +```{r} +#| label: "model" + +# Filter out peptides with low signals +high_peptides <- krsa_filter_lowPeps(data_pw_max, 5L) + +# Fit the linear model +# This will produce a list of data frames: +# scaled: the Slope_Transformed values (see above for more info on Slope_Transformed) +# normalized: the Slope_Transformed values but normalized by Chip/Barcode +# Grouped: The mean of Slope_Transformed values for all samples within a group +data_modeled <- krsa_scaleModel(data_pw, high_peptides) + +# Filter out peptides weak linear fit +linear_peptides <- krsa_filter_nonLinear(data_modeled[["scaled"]], 0.8) + +# Filter out reference peptides +clean_peptides <- krsa_filter_ref_pep(linear_peptides) +``` + +## Global Signal Intensity + +For a global signal intensity across all samples/groups, few figures can be plotted based on the *Slope_Transformed* values. + +### Global CV Plots + +We will plot the coefficient of variation on both the normal and normalized fits. This will help us to identify groups with high variation that could be explained by sample outliers. + +```{r} +#| label: "global-cv-plot" +#| fig-width: 6 +#| fig-height: 6 +#| fig-cap: "Coefficient of Variation plotted for each peptide across all 4 groups" +#| eval: !expr params$pairwise +if(!is.null(params[["figure_output"]])) dir.create(params[["figure_output"]]) +# Plot a CV figure using the modeled normalized data +if (!is.null(params[["figure_output"]])) { + krsa_cv_plot(data_modeled[["normalized"]], clean_peptides) |> ggsave(filename=paste(params[["figure_output"]], "cv_plot.pdf", sep="/")) + } else { + krsa_cv_plot(data_modeled[["normalized"]], clean_peptides) + } + +``` + +### Global Violin Plots + +We will plot violin figures to examine global signal differences between groups/samples. + +```{r} +#| label: "global-violin-plot-by-group" +#| fig-width: 6 +#| fig-height: 6 +#| fig-cap: "Violin Plots for signal intensity Distribution Across Groups for all replicates" + +# Plot a violin figure and facet by the (Group) variable + +if (!is.null(params[["figure_output"]])) { + krsa_violin_plot(data_modeled[["scaled"]], clean_peptides, "Group") |> ggsave(filename=paste(params[["figure_output"]], "violin_plot_bygroup.pdf", sep="/")) + } else { + krsa_violin_plot(data_modeled[["scaled"]], clean_peptides, "Group") + } + +``` + +```{r} +#| label: "global-violin-plot-by-chip" +#| fig-width: 6 +#| fig-height: 6 +#| fig-cap: "Violin Plots for signal intensity Distribution Across Chips for all replicates" + +# Plot a violin figure and facet by the (Barcode) variable +if (!is.null(params[["figure_output"]])) { + krsa_violin_plot(data_modeled[["scaled"]], clean_peptides, "Barcode") |> ggsave(filename=paste(params[["figure_output"]], "violin_plot_bybarcode.pdf", sep="/")) + } else { + krsa_violin_plot(data_modeled[["scaled"]], clean_peptides, "Barcode") + } + +``` + +### Global Heatmaps + +The heatmap represent all the peptides present on the chip except the positive/internal controls and peptides that failed to pass QC. The heatmaps are scaled by row to highlight the peptide signal differences across the samples. A hierarchical unsupervised clustering is applied both on the peptides and the samples to group potentially similar signatures. + +```{r} +#| label: "global-signal-heatmap-individual" +#| fig-cap: "Row and chip normalized intensity values for the selected peptides" +#| fig-height: 6 +#| fig-width: 6 + +# Generates a heatmap using the modeled normalized data +nheatmap <- krsa_heatmap(data_modeled[["normalized"]], clean_peptides, scale = "row") +if (!is.null(params[["figure_output"]])) { + graphics.off() + pdf(file = paste(params[["figure_output"]], "normalized_heatmap.pdf", sep="/")) + grid::grid.newpage() + grid::grid.draw(nheatmap$gtable) + dev.off() +} else { + grid::grid.newpage() + grid::grid.draw(nheatmap$gtable) +} + +``` + +```{r} +#| label: "global-signal-heatmap-grouped" +#| fig-cap: "Row and group normalized intensity values for the selected peptides" +#| fig-height: 6 +#| fig-width: 6 + +# Generates a heatmap using the modeled grouped data +gheatmap <- krsa_heatmap_grouped(data_modeled[["grouped"]], clean_peptides, scale = "row") +if (!is.null(params[["figure_output"]])) { + graphics.off() + pdf(file = paste(params[["figure_output"]], "normalized_grouped_heatmap.pdf", sep="/")) + grid::grid.newpage() + grid::grid.draw(gheatmap$gtable) + dev.off() +} else { + grid::grid.newpage() + grid::grid.draw(gheatmap$gtable) +} +``` + +## Group Comparison + +To compare between samples, a two-group comparison is performed. In this case, there are three group comparisons: + +- Case 1 vs Control 1 + +The *Slope_Transformed* ratio between each group, paired by chip, is calculated to the fold change. Based on the fold change, peptides that pass a certain fold change threshold are considered significant hits. Also, quality control steps applied in each comparison to filter out peptides that do not reach specific criteria: + +- The *Median_SigmBg* at max exposure *100ms* must be above a certain value\ +- $R^2$ of the linear model fit must be above a threshold value + +These *Filtering Parameters* (fold change threshold, QC criteria) can be modified to adjust the stringency of the analysis. The *Filtering Parameters* that are used for this analysis: + +- The *Median_SigmBg* at max exposure *100ms* must be equal or above 5\ +- $R^2$ of the linear model fit must be above or equal 0.8\ +- Log fold change (LFC) cutoffs at (0.2,0.3,0.4) + +```{r} +#| label: "group-comp-list" + +# You can define the comparison groups here. +# The group names are in the `groups` variable and you can use subscripting to select the groups +# For example, to compare the first and fourth groups, you can use `groups[1]` and `groups[4]` +# Then copy the next block as many times as there are comparisons and change the `COMP1` to `COMP2`, `COMP3`, etc. +# Also make sure that the label is unique for each block + +# Define Groups to be compared +comparisons <- list( + COMP1 = c(groups[[1L]], groups[[2L]]), + COMP2 = c(groups[[1L]], groups[[3L]]), + COMP3 = c(groups[[1L]], groups[[4L]]), + COMP4 = c(groups[[2L]], groups[[3L]]), + COMP5 = c(groups[[2L]], groups[[4L]]), + COMP6 = c(groups[[3L]], groups[[4L]]) +) +``` + +```{r} +#| results: "asis" +#| echo: FALSE +#| include: TRUE +#| label: "childA" +#| fig-width: 6 +#| fig-height: 6 +#| fig-cap: "Heatmap of pairwise group comparison" + +case <- comparisons[["COMP1"]][[1L]] +ctrl <- comparisons[["COMP1"]][[2L]] +random <- "A" +threshold <- params[["threshold"]] + +src <- knitr::knit_expand( + file = "_comparison.Rmd", + case = case, ctrl = ctrl, threshold = threshold, + figure_output = params[["figure_output"]], + chip = params[["chip_type"]] +) + +res <- knitr::knit_child(text = src, quiet = TRUE) + +cat(res, sep = "\n") +``` +# Session Info + +```{r session_info} +devtools::session_info() +``` +