Skip to content

Commit 4e80d0f

Browse files
realmarcinclaude
andauthored
Wire M-P coculture GEM paper into Methylomicrobium-Chlorella community (#63)
* Wire Badr/He/Wang M-P coculture GEM paper into Methylomicrobium-Chlorella doi:10.20517/mrr.2023.70 (Badr/He/Wang 2023, "Probing interspecies metabolic interactions within a synthetic binary microbiome using genome-scale modeling") is an orphan cache that develops three genome-scale modeling approaches - SteadyCom, dynamic FBA Lab, and their new DynamiCom - on a methanotroph-photoautotroph (M-P) coculture model system. Wire it as PARTIAL evidence on the Methane-and-Carbon-Dioxide-Mitigation cross-feeding interaction in the existing Methylomicrobium-Chlorella community: same kind of methanotroph-microalga consortium as the curated record, and the abstract directly speaks to "the metabolic interactions within the coculture, how they contribute to the enhanced growth observed in the coculture" - which is the central claim of that interaction. PARTIAL because the modeled M-P system in the paper is generic rather than the specific M. alcaliphilum 20Z + Chlorella HS2 pairing curated here. Co-Authored-By: Claude Opus 4.7 (1M context) <noreply@anthropic.com> * Address Copilot review on PR #63 - Move the GEM-modeling evidence off the Methane-and-Carbon-Dioxide- Mitigation interaction and onto Saline-Metabolic-Coupling-and-pH- Recovery. The paper's central claims are about metabolic coupling driving enhanced coculture growth, not CH4/CO2 mitigation or biomass carbon sequestration; the new placement aligns the evidence with what the snippet actually supports ("robust metabolic coupling between the two microbes" is the lead evidence on this interaction). - Author/year correction: "Badr/He/Wang 2023" -> "Badr/He/Wang 2024" to match the journal's 2024-05-27 publication date in Crossref (the "2023" in the DOI path 10.20517/mrr.2023.70 is the volume/issue stamp, not the publication year). Co-Authored-By: Claude Opus 4.7 (1M context) <noreply@anthropic.com> --------- Co-authored-by: Claude Opus 4.7 (1M context) <noreply@anthropic.com>
1 parent a5392dc commit 4e80d0f

1 file changed

Lines changed: 12 additions & 0 deletions

File tree

kb/communities/Methylomicrobium_Chlorella_Methane_Sequestration_Coculture.yaml

Lines changed: 12 additions & 0 deletions
Original file line numberDiff line numberDiff line change
@@ -237,6 +237,18 @@ ecological_interactions:
237237
evidence_source: IN_VITRO
238238
snippet: pH recovery above mild alkaline level was observed in co-cultures.
239239
explanation: Supports pH recovery as a coculture-level outcome.
240+
- reference: doi:10.20517/mrr.2023.70
241+
supports: PARTIAL
242+
evidence_source: COMPUTATIONAL
243+
snippet: methanotroph-photoautotroph (M-P) coculture..the metabolic interactions
244+
within the coculture, how they contribute to the enhanced growth observed in the
245+
coculture
246+
explanation: Badr/He/Wang 2024 use a generic methanotroph-photoautotroph (M-P)
247+
coculture as their model system and demonstrate via genome-scale modeling that the
248+
mutualistic N-exchange between methanotroph and cyanobacteria explains enhanced
249+
coculture growth - independent computational backing for "metabolic coupling between
250+
the two microbes" claimed here. Partial because the modeled M-P system is generic
251+
rather than the specific M. alcaliphilum 20Z + Chlorella HS2 pairing.
240252
environmental_factors:
241253
- name: Salinity
242254
value: above 3.0%

0 commit comments

Comments
 (0)