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Wire 2 SPRUCE-specific orphan caches into SPRUCE community (#62)
* Wire 2 more SPRUCE-specific orphan caches into SPRUCE community Both orphan papers explicitly cite the SPRUCE Spruce and Peatland Responses Under Changing Environments experiment in northern Minnesota that is the namesake of SPRUCE_Peatland_Methane_Cycling_ Community. - PMID:34836550 (ter Horst et al. 2021 Microbiome, "Minnesota peat viromes reveal terrestrial and aquatic niche partitioning"): 87 metagenomes + 5 viromes from the SPRUCE warming experiment and surrounding bog. Wired as evidence on the methanogenic archaea taxon - the SPRUCE viral community interacts with the methanogenic populations already characterized in the community. - PMID:38515239 (Bourgault et al. 2024 New Phytologist, "Responses of vascular plant fine roots and associated microbial communities to whole-ecosystem warming and elevated CO2 in northern peatlands"): explicitly uses SPRUCE (+0 to +9 deg C warming and eCO2) to characterize the fine-root rhizosphere microbiome. Wired as additional evidence on the existing whole-ecosystem warming environmental factor. Both papers were orphan caches with full PubMed abstracts on disk but no community citing them; their abstracts mention SPRUCE by name in their first paragraph, so the citation match is unambiguous. Co-Authored-By: Claude Opus 4.7 (1M context) <noreply@anthropic.com> * Address Copilot review on PR #62 - Move the PMID:34836550 evidence off the methanogenic-archaea taxon and onto the Acidic-organic-rich-peat-chemistry environmental factor. The original snippet was about site sampling, not methanogens, so the taxon placement was a scope mismatch. The replacement snippet ("viral community composition was significantly correlated with peat depth, water content, and carbon chemistry, including CH4 and CO2 concentrations") directly supports peat chemistry as a driver of community composition, which is the central claim of that environmental factor. - Author attribution: "ter Horst" -> "Ter Horst" at sentence start to match citation formatting. Co-Authored-By: Claude Opus 4.7 (1M context) <noreply@anthropic.com> --------- Co-authored-by: Claude Opus 4.7 (1M context) <noreply@anthropic.com>
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kb/communities/SPRUCE_Peatland_Methane_Cycling_Community.yaml

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@@ -139,6 +139,16 @@ environmental_factors:
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snippet: The Spruce and Peatland Responses Under Changing Environments (SPRUCE) experiment, combines
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air and peat warming in a whole-ecosystem manipulation experiment
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explanation: Supports the major experimental perturbation applied to the natural peatland community.
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- reference: PMID:38515239
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supports: SUPPORT
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evidence_source: IN_VIVO
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snippet: the Spruce and Peatland Responses Under Changing Environments (SPRUCE) experiment,
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to explore the effects of a whole-ecosystem warming gradient (+0°C to 9°C) and
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eCO2 on vascular plant fine roots and their associated microbes
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explanation: Bourgault et al. 2024 New Phytologist independently confirms the SPRUCE
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warming/+eCO2 design and extends it to vascular plant fine roots and their
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associated rhizosphere microbes - linking the manipulation to the plant-root side
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of the same peatland system.
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- name: Acidic organic-rich peat chemistry
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value: pH 3.42 average outflow; total organic carbon 85.83 mg C/L
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description: The bog porewater chemistry is acidic and organic-carbon rich, consistent with an oligotrophic
@@ -149,6 +159,15 @@ environmental_factors:
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evidence_source: IN_VIVO
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snippet: the pH was 3.42 and total organic carbon was 85.83 mg C/L.
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explanation: Supports acidic, organic-rich conditions in the SPRUCE peatland system.
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- reference: PMID:34836550
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supports: SUPPORT
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evidence_source: COMPUTATIONAL
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snippet: viral community composition was significantly correlated with peat depth,
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water content, and carbon chemistry, including CH4 and CO2 concentrations
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explanation: Ter Horst et al. 2021 link the resident dsDNA viral community at SPRUCE
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to the peatland's CH4/CO2 carbon-chemistry gradient and depth/water structure -
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reinforcing peat chemistry (alongside the existing pH/TOC quantification) as a
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principal driver of community composition.
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associated_datasets:
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- name: SPRUCE peat metagenomes
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dataset_type: METAGENOME

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