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realmarcinclaude
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Wire 2 more orphan caches into existing communities (Stordalen, CPR) (#61)
* Wire 2 more orphan caches into existing communities - Stordalen_Mire_Methylotrophic_Methanogenesis_Community: add the Singleton/Tyson 2018 Nature permafrost paper (PMID:30013118) as orthogonal supporting evidence. Same Stordalen Mire thaw gradient, same site, and the 1,529-MAG genome-resolved catalog directly backs the genome-resolved-microbiome framing of this community, plus the abstract's mention of "novel syntrophic relationships" supports the SYNTROPHY interaction. - Episymbiotic_CPR_DPANN_Groundwater_Community: add Tian et al. 2020 Microbiome ("Small and mighty: adaptation of superphylum Patescibacteria to groundwater environment", PMID:32252814) as orthogonal CPR taxonomic backing. Genome-resolved characterization of the same Patescibacteria superphylum this community describes, also in groundwater. Both papers were orphan caches not cited by any community before. Co-Authored-By: Claude Opus 4.7 (1M context) <noreply@anthropic.com> * Address Copilot review on PR #61 - Stordalen_Mire: fix two attribution + scope errors flagged by Copilot. - "Singleton/Tyson 2018" -> "Woodcroft et al. 2018" (Woodcroft is first author; Tyson is last/PI). - Drop the "same Stordalen Mire" claim in both explanations - the abstract describes the thaw gradient generically and does not name Stordalen Mire (that's in the paper body). Downgrade to PARTIAL and explicitly note the gap in scope. - Episymbiotic_CPR_DPANN_Groundwater: replace the over-short "superphylum Patescibacteria" snippet with a longer verbatim excerpt that links Patescibacteria to groundwater wells via 16S amplicon + metagenomic sequencing: "wells over time, we sequenced the 16S rRNA gene of 214 samples as well as the metagenomes of 12 representative wells to identify features unique to the Patescibacteria". Explanation rewritten to match what the snippet actually supports. Co-Authored-By: Claude Opus 4.7 (1M context) <noreply@anthropic.com> --------- Co-authored-by: Claude Opus 4.7 (1M context) <noreply@anthropic.com>
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kb/communities/Episymbiotic_CPR_DPANN_Groundwater_Community.yaml

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snippet: The pristine sites, which serve as local sources of drinking water,
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contained up to 31% CPR bacteria
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explanation: Supports CPR membership and high relative abundance.
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- reference: PMID:32252814
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supports: SUPPORT
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evidence_source: COMPUTATIONAL
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snippet: wells over time, we sequenced the 16S rRNA gene of 214 samples as well
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as the metagenomes of 12 representative wells to identify features unique to
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the Patescibacteria
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explanation: Tian et al. 2020 ("Small and mighty") characterize Patescibacteria
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via 16S amplicon + metagenomic sequencing of groundwater wells - directly anchors
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the groundwater Patescibacteria/CPR framing of this community.
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- taxon_term:
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preferred_term: groundwater DPANN archaea
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term:

kb/communities/Stordalen_Mire_Methylotrophic_Methanogenesis_Community.yaml

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evidence_source: IN_VIVO
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snippet: across all stages of a permafrost thaw gradient in Stordalen
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explanation: Supports the palsa-bog-fen thaw-gradient sampling context.
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- reference: PMID:30013118
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supports: PARTIAL
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evidence_source: COMPUTATIONAL
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snippet: metagenomic sequencing of 214 samples from a permafrost thaw gradient
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to recover 1,529 metagenome-assembled genomes
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explanation: Woodcroft et al. 2018 Nature paper establishes a 1,529-MAG genome-resolved
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catalog from a permafrost thaw gradient. Partial because the abstract describes
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the gradient generically rather than naming Stordalen Mire specifically - that site
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identification is in the paper body, not the abstract.
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- taxon_term:
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preferred_term: Stordalen Mire methanogenic archaea
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term:
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evidence_source: COMPUTATIONAL
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snippet: co-occurrence of methylotrophy throughout
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explanation: Supports methylotrophy as a co-occurring methanogenesis mode.
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- reference: PMID:30013118
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supports: PARTIAL
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evidence_source: COMPUTATIONAL
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snippet: Microbial and geochemical data highlight lineages that correlate with
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the production of greenhouse gases and indicate novel syntrophic relationships
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explanation: Woodcroft et al. 2018 establish novel syntrophic relationships and
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lineage-greenhouse-gas correlations in a permafrost thaw gradient, supporting
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the SYNTROPHY framing of methanogenic methylotrophy at the community level.
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Partial because the abstract does not name Stordalen Mire specifically.
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- name: Bacterial Anaerobic Methylotrophy
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description: Bacterial MAGs encode and express anaerobic methylotrophic
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potential across Stordalen Mire, indicating a bacterial route for processing

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