diff --git a/packages/dd-trace/src/aiguard/sdk.js b/packages/dd-trace/src/aiguard/sdk.js
index 2ca68eb18a..b9c87c2b19 100644
--- a/packages/dd-trace/src/aiguard/sdk.js
+++ b/packages/dd-trace/src/aiguard/sdk.js
@@ -63,6 +63,18 @@ class AIGuardClientError extends Error {
   }
 }
 
+/**
+ * Resolves the AI Guard host for a given Datadog site. Sites with a single subdomain level
+ * (e.g. `datadoghq.com`, `ddog-gov.com`) are served from the `app.` subdomain, while regional
+ * sites (e.g. `us3.datadoghq.com`, `ap1.datadoghq.com`) are used as-is.
+ *
+ * @param {string} site - Datadog site (e.g. `datadoghq.com`, `us3.datadoghq.com`)
+ * @returns {string} The host to use for the AI Guard endpoint
+ */
+function aiGuardHost (site) {
+  return site.split('.').length === 2 ? `app.${site}` : site
+}
+
 class AIGuard extends NoopAIGuard {
   #initialized
   #tracer
@@ -94,7 +106,7 @@ class AIGuard extends NoopAIGuard {
       'DD-AI-GUARD-SOURCE': 'SDK',
       'DD-AI-GUARD-LANGUAGE': 'nodejs',
     }
-    const endpoint = config.experimental.aiguard.endpoint || `https://app.${config.site}/api/v2/ai-guard`
+    const endpoint = config.experimental.aiguard.endpoint || `https://${aiGuardHost(config.site)}/api/v2/ai-guard`
     this.#evaluateUrl = `${endpoint}/evaluate`
     this.#timeout = config.experimental.aiguard.timeout
     this.#maxMessagesLength = config.experimental.aiguard.maxMessagesLength
diff --git a/packages/dd-trace/test/aiguard/index.spec.js b/packages/dd-trace/test/aiguard/index.spec.js
index 3df963b689..d2dfaa5061 100644
--- a/packages/dd-trace/test/aiguard/index.spec.js
+++ b/packages/dd-trace/test/aiguard/index.spec.js
@@ -523,11 +523,14 @@ describe('AIGuard SDK', () => {
   const sites = [
     { site: 'datad0g.com', endpoint: 'https://app.datad0g.com/api/v2/ai-guard' },
     { site: 'datadoghq.com', endpoint: 'https://app.datadoghq.com/api/v2/ai-guard' },
+    { site: 'ddog-gov.com', endpoint: 'https://app.ddog-gov.com/api/v2/ai-guard' },
+    { site: 'us3.datadoghq.com', endpoint: 'https://us3.datadoghq.com/api/v2/ai-guard' },
+    { site: 'ap1.datadoghq.com', endpoint: 'https://ap1.datadoghq.com/api/v2/ai-guard' },
   ]
   for (const { site, endpoint } of sites) {
     it(`test endpoint discovery: ${site}`, async () => {
-      const newConfig = { site, ...config }
-      delete newConfig.experimental.aiguard.endpoint
+      const { endpoint: _discardedEndpoint, ...aiguard } = config.experimental.aiguard
+      const newConfig = { ...config, site, experimental: { ...config.experimental, aiguard } }
       const client = new AIGuard(tracer, newConfig)
       mockFetch({
         body: { data: { attributes: { action: 'ALLOW', reason: 'OK', is_blocking_enabled: false } } },