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Fix binning
1 parent 89f3c35 commit e717bf8

1 file changed

Lines changed: 8 additions & 7 deletions

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src/cryoemservices/services/extract_subtomo.py

Lines changed: 8 additions & 7 deletions
Original file line numberDiff line numberDiff line change
@@ -184,10 +184,7 @@ def extract_subtomo(self, rw, header: dict, message: dict):
184184
tilt_numbers.append(_get_tilt_number_v5_12(Path(tilt_name)))
185185
tilt_axis_from_file = float(_get_tilt_angle_v5_12(Path(tilt_name)))
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tilt_angles_radians.append(
187-
(
188-
tilt_axis_from_file
189-
+ extract_subtomo_params.refined_tilt_offset
190-
)
187+
(tilt_axis_from_file + extract_subtomo_params.tilt_offset)
191188
* np.pi
192189
/ 180
193190
)
@@ -215,6 +212,7 @@ def extract_subtomo(self, rw, header: dict, message: dict):
215212
theta_z=tilt_axis_radians,
216213
delta_x=x_shifts[tilt],
217214
delta_y=y_shifts[tilt],
215+
binning=extract_subtomo_params.tomogram_binning,
218216
)
219217
if tilt_angles_radians[tilt] == 0:
220218
with open(
@@ -370,20 +368,23 @@ def get_coord_in_tilt(
370368
theta_z: float,
371369
delta_x: float,
372370
delta_y: float,
371+
binning: int,
373372
):
373+
# In binned coordinates here
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x_centred = x - cen_x
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y_centred = y - cen_y + cen_x * np.tan(theta_z) # TODO: last factor depends on rot
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z_centred = z - cen_z
377377
x_2d = (
378378
x_centred * np.cos(theta_z) * np.cos(theta_y)
379379
- y_centred * np.sin(theta_z)
380380
+ z_centred * np.cos(theta_z) * np.sin(theta_y)
381-
+ delta_x
382381
)
383382
y_2d = (
384383
x_centred * np.sin(theta_z) * np.cos(theta_y)
385384
+ y_centred * np.cos(theta_z)
386385
+ z_centred * np.sin(theta_z) * np.sin(theta_y)
387-
+ delta_y
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)
389-
return cen_x + x_2d, cen_y + y_2d
387+
# Un-bin and apply shifts
388+
x_tilt = (cen_x + x_2d) * binning - delta_x
389+
y_tilt = (cen_y + y_2d) * binning - delta_y
390+
return x_tilt, y_tilt

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