@@ -355,40 +355,3 @@ bambu.singlecell <- function(reads, annotations = NULL, genome = NULL, NDR = NUL
355355 quantData = quantData , dedupUMI = dedupUMI , clusters = clusters ,
356356 processByChromosome = processByChromosome , processByBam = processByBam )
357357}
358-
359- # ' Spatial wrapper for bambu
360- # ' @title Spatial isoform reconstruction and quantification
361- # ' @description Wrapper around \code{\link{bambu}} for spatial transcriptomics
362- # ' data. Accepts an \code{opt.spatial} list for spatial specific parameters
363- # ' and passes all other arguments directly to \code{\link{bambu}}.
364- # ' @param opt.spatial A list of spatial specific parameters:
365- # ' \describe{
366- # ' \item{extractBarcodeUMI}{Logical, whether to extract spot barcodes and
367- # ' UMIs from BAM tags or read names. Defaults to FALSE}
368- # ' \item{dedupUMI}{Logical, whether to perform UMI-based deduplication per
369- # ' barcode. Defaults to FALSE}
370- # ' \item{clusters}{A named list mapping cluster names to barcode vectors,
371- # ' used for cluster-level transcript discovery. Defaults to NULL}
372- # ' }
373- # ' @inheritParams bambu
374- # ' @export
375- bambu.spatial <- function (reads , annotations = NULL , genome = NULL , NDR = NULL ,
376- mode = NULL , opt.discovery = NULL , opt.em = NULL , opt.spatial = NULL ,
377- rcOutDir = NULL , discovery = TRUE , assignDist = TRUE , quant = TRUE ,
378- stranded = FALSE , ncore = 1 , yieldSize = NULL , trackReads = FALSE ,
379- returnDistTable = FALSE , lowMemory = FALSE , sampleData = NULL ,
380- fusionMode = FALSE , verbose = FALSE , quantData = NULL ,
381- processByChromosome = FALSE , processByBam = TRUE ) {
382- extractBarcodeUMI <- isTRUE(opt.spatial $ extractBarcodeUMI )
383- dedupUMI <- isTRUE(opt.spatial $ dedupUMI )
384- clusters <- opt.spatial $ clusters
385- bambu(reads = reads , annotations = annotations , genome = genome , NDR = NDR ,
386- mode = mode , opt.discovery = opt.discovery , opt.em = opt.em ,
387- rcOutDir = rcOutDir , discovery = discovery , assignDist = assignDist ,
388- quant = quant , stranded = stranded , ncore = ncore , yieldSize = yieldSize ,
389- trackReads = trackReads , returnDistTable = returnDistTable ,
390- lowMemory = lowMemory , sampleData = sampleData , fusionMode = fusionMode ,
391- verbose = verbose , extractBarcodeUMI = extractBarcodeUMI ,
392- quantData = quantData , dedupUMI = dedupUMI , clusters = clusters ,
393- processByChromosome = processByChromosome , processByBam = processByBam )
394- }
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