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Auto-generated via `{sandpaper}` Source : 9247f49 Branch : main Author : Stella Prins <30465823+stellaprins@users.noreply.github.com> Time : 2026-06-09 10:49:18 +0000 Message : Merge pull request #130 from HealthBioscienceIDEAS/sp/typos Fix typos found with codespell
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choosing-acquisition-settings.md

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::::::::::::::::::::::::::::::::::::: challenge
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## Choosing acquisiton settings
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## Choosing acquisition settings
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In the previous episode, we used an example of investigating the effects of a
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specific chemical on cells grown in culture. Continuing with this, which
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micrometre wide) overlaid by a 5 micrometre pixel grid.
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Right - the equivalent image with a grayscale colormap" width="80%"}
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Failing to meet the Nyquist critera can also result in various image artifacts
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Failing to meet the Nyquist criteria can also result in various image artifacts
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known as 'aliasing'. For example, consider the digram below - here we have 6
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small cells (blue) that are evenly spaced in the x direction. Using a pixel
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spacing of 10 micrometre, this produces an odd effect in our final image, where

designing-a-light-microscopy-experiment.md

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| :----- | :------- | :-------
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| Brightfield | Illuminates the sample with light from one side, and images on the other | Hard to see many biological structures - usually requires contrast agents/staining to increase contrast
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| Phase contrast / DIC | Makes use of slight changes in the ‘phase’ of light as it passes through a sample to increase contrast | Allows unstained samples to be seen more easily
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| Widefield flourescence | Fluorescent labels (with specific excitation and emission wavelengths) bind to specific biological structures | Widefield illuminates the whole sample at once, which can lead to blurry images in thicker samples
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| Widefield fluorescence | Fluorescent labels (with specific excitation and emission wavelengths) bind to specific biological structures | Widefield illuminates the whole sample at once, which can lead to blurry images in thicker samples
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| Laser scanning confocal | A laser is scanned across the sample point by point in a raster pattern | Allows 'optical sectioning', giving clearer images in full 3D. More complex to use than widefield, also slower to acquire images.
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| Spinning disc confocal | An array of pinholes on a disc split the laser into hundreds of beams that move across the sample | Faster than standard laser scanning confocal
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| Super-resolution | Wide range of methods that break the classic 'diffraction limit' of light, allowing resolutions down to tens of nanometres | More complex to use than standard widefield / confocal

filetypes-and-metadata.md

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If you want to open more types of file, you will have to install the relevant
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reader following the
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[intructions in the BioIO docs](https://bioio-devs.github.io/bioio/OVERVIEW.html).
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[instructions in the BioIO docs](https://bioio-devs.github.io/bioio/OVERVIEW.html).
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Note the `bioio-bioformats` reader should allow a wide range of file formats to
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be read.
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imaging-software.md

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a piece of software (rather than automating use of existing features). They
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allow members of the community, outside the main team that develops the
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software, to add features they need for a particular image type or processing
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task. They're designed to be re-useable so other members of the community can
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task. They're designed to be reusable so other members of the community can
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easily benefit from these new features.
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::::::::::::::::::::::::::::::::::::::::::::::::
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You should see a fluorescence microscopy image of some cells:
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![](fig/cells-napari.png){alt="A screenshot of a flourescence microscopy image
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![](fig/cells-napari.png){alt="A screenshot of a fluorescence microscopy image
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of some cells in Napari"}
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## Napari's User interface

instance-segmentation-and-measurements.md

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- How do we perform instance segmentation in Napari?
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- How do we measure cell size with Napari?
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- How do we save our work to create re-usable workflows?
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- How do we save our work to create reusable workflows?
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::::::::::::::::::::::::::::::::::::::::::::::::
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- Calculate the number of cells and average cell volume.
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- Save and edit your workflow to re-use on subsequent images.
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- Save and edit your workflow to reuse on subsequent images.
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- Perform more complex cell shape analysis using scikit-image's `regionprops`.
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viewer.layers.remove('instance_seg')
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eroded_semantic_seg = viewer.layers['eroded ball 10'].data
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# Create a new instace segmentation using the eroded mask
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# Create a new instance segmentation using the eroded mask
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instance_seg = label(eroded_semantic_seg)
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viewer.add_labels(instance_seg)
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You now have a correct instance segmentation. You could return to
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using the napari-skimage plugin to calculate the sizes
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of each nucleus and export the results to a speadsheet or your preferred
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of each nucleus and export the results to a spreadsheet or your preferred
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analysis software using the `Save Results` function. However you've probably
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picked up enough Python during this course to complete the analysis you need
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with just the Napari console. Let's give it a try. The following commands
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volume of cell nuclei suitable for an experiment investigating how these
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We can save our work from the console for re-use on data from subsequent
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We can save our work from the console for reuse on data from subsequent
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time points, creating a repeatable measurement workflow.
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```python

md5sum.txt

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"index.md" "033b7f76a4ec3e321923969b2b53e0f4" "site/built/index.md" "2026-05-05"
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"links.md" "8184cf4149eafbf03ce8da8ff0778c14" "site/built/links.md" "2024-08-16"
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"episodes/imaging-software.md" "cd1002354eaf1d92c83ca9b235443593" "site/built/imaging-software.md" "2025-06-25"
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"episodes/what-is-an-image.md" "77453b0c3031890f96104617bcd46a49" "site/built/what-is-an-image.md" "2025-11-04"
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"episodes/image-display.md" "0d8150fdf6516bc2abb026debedfa3dd" "site/built/image-display.md" "2025-11-04"
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"episodes/multi-dimensional-images.md" "36d8d27a775bf837aaa3cf77791779fa" "site/built/multi-dimensional-images.md" "2026-06-08"
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"episodes/filetypes-and-metadata.md" "719c5682d05a8c1a15079df7fd782137" "site/built/filetypes-and-metadata.md" "2026-06-08"
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"episodes/filetypes-and-metadata.md" "09d357ef062974fade04f70e0fc7a28c" "site/built/filetypes-and-metadata.md" "2026-06-09"
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"episodes/quality-control-and-manual-segmentation.md" "53ab52caf27354a2c90878a8d0eeb650" "site/built/quality-control-and-manual-segmentation.md" "2026-06-09"
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multi-dimensional-images.md

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data from different 'channels'. Remove the brain image and select:
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are common examples of images with multiple channels. In fluorescence
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microscopy, different 'flourophores' are used that target specific features
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quality-control-and-manual-segmentation.md

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It's worth bearing in mind that automated methods are rarely perfect (whether
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they're classic image processing, machine learning or deep learning based). It's

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