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differences for PR #124
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fig/metadata-console.png

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filetypes-and-metadata.md

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```
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```output
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OME(
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experimenters=[{'id': 'Experimenter:0', 'user_name': 'Zeiss'}],
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instruments=[<1 field_type>],
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images=[<1 field_type>],
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plates=[<1 field_type>],
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experimenters=[{'id': 'Experimenter:Zeiss', 'user_name': 'Zeiss'}],
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instruments=[<1 field_type>],
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images=[<2 field_type>],
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structured_annotations={
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'xml_annotations': [
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{
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'description': 'ZEN 2012 (blue edition)1.1.1.0',
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'id': 'urn:lsid:allencell.org:Annotation:AcquisitionSoftware',
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'value': {},
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'kind': 'xmlannotation'
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}
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]
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}
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)
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```
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In this case, we can see it is split into three categories:
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In this case, we can see it is split into various categories including
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`experimenters`, `images` and `instruments`. You can look inside each of these
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using `.category` e.g.:
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```python
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Explore the metadata in the console to answer the following
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questions:
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- What type of microscope was used to take this image?
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- Which manufacturer made the microscope used to take this image?
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- What detector was used to take this image?
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- What does each channel show? For example, which fluorophore is used? What is
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its excitation and emission wavelength? (hint: look inside the image's
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## Solution
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### Microscope model
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### Microscope manufacturer
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Under `metadata.instruments[0].microscope`, we can see that an 'Axio Imager Z2'
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microscope was used.
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Under `metadata.instruments[0].microscope`, we can see that the microscope
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manufacturer was Zeiss.
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### Detector
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Under `metadata.instruments[0].detectors`, we can see
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### Channels
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Under `metadata.images[0].pixels.channels`, we can see one entry per channel
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- `Channel:0:0`, `Channel:0:1` and `Channel:0:2`.
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- `Channel:1:0`, `Channel:2:0` and `Channel:3:0`.
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In the first (`metadata.images[0].pixels.channels[0]`), we can see that its
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`name` is TagYFP, a fluorophore with `emission_wavelength` of 524nm and
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`fluor` is TagYFP, a fluorophore with `emission_wavelength` of 524nm and
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`excitation_wavelength` of 508nm.
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In the first (`metadata.images[0].pixels.channels[1]`), we can see that its
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`name` is mRFP1.2, a fluorophore with `emission_wavelength` of 612nm and
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In the second (`metadata.images[0].pixels.channels[1]`), we can see that its
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`fluor` is mRFP1.2, a fluorophore with `emission_wavelength` of 612nm and
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`excitation_wavelength` of 590nm.
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In the first (`metadata.images[0].pixels.channels[2]`), we see that its name
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is `TL DIC` and no `emission_wavelength` or `excitation_wavelength` is listed.
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In the last (`metadata.images[0].pixels.channels[2]`), we see that no `fluor`,
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`emission_wavelength` or `excitation_wavelength` is listed.
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Its `illumination_type` is listed as 'transmitted', so this is simply an image
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of the yeast cells with no fluorophores used.
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md5sum.txt

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"episodes/what-is-an-image.md" "77453b0c3031890f96104617bcd46a49" "site/built/what-is-an-image.md" "2025-11-04"
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"episodes/image-display.md" "0d8150fdf6516bc2abb026debedfa3dd" "site/built/image-display.md" "2025-11-04"
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"episodes/multi-dimensional-images.md" "e723d61ca973be7d6e8ea118fa4679a5" "site/built/multi-dimensional-images.md" "2025-10-29"
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"episodes/filetypes-and-metadata.md" "da960d27ce829282421cd9d27c223821" "site/built/filetypes-and-metadata.md" "2025-10-29"
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"episodes/filetypes-and-metadata.md" "1407632ccab788a7820067e7fd5969cf" "site/built/filetypes-and-metadata.md" "2026-06-08"
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"episodes/designing-a-light-microscopy-experiment.md" "9a236fbc9a48f560a786077ea9662c9c" "site/built/designing-a-light-microscopy-experiment.md" "2025-11-04"
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"episodes/choosing-acquisition-settings.md" "1bf362eeea03fe66d4e170d9803dc649" "site/built/choosing-acquisition-settings.md" "2024-08-16"
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"episodes/quality-control-and-manual-segmentation.md" "f4f883be70cd5f8cf8f24d32815064b5" "site/built/quality-control-and-manual-segmentation.md" "2026-06-05"

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