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4 changes: 2 additions & 2 deletions episodes/choosing-acquisition-settings.md
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Expand Up @@ -304,7 +304,7 @@ settings, with figure 5 as a great example of the different trade-offs.

::::::::::::::::::::::::::::::::::::: challenge

## Choosing acquisiton settings
## Choosing acquisition settings

In the previous episode, we used an example of investigating the effects of a
specific chemical on cells grown in culture. Continuing with this, which
Expand Down Expand Up @@ -541,7 +541,7 @@ capture the two cells, no matter their alignment with the grid:
micrometre wide) overlaid by a 5 micrometre pixel grid.
Right - the equivalent image with a grayscale colormap" width="80%"}

Failing to meet the Nyquist critera can also result in various image artifacts
Failing to meet the Nyquist criteria can also result in various image artifacts
known as 'aliasing'. For example, consider the digram below - here we have 6
small cells (blue) that are evenly spaced in the x direction. Using a pixel
spacing of 10 micrometre, this produces an odd effect in our final image, where
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2 changes: 1 addition & 1 deletion episodes/designing-a-light-microscopy-experiment.md
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Expand Up @@ -421,7 +421,7 @@ choosing image processing and statistical analysis methods.
| :----- | :------- | :-------
| Brightfield | Illuminates the sample with light from one side, and images on the other | Hard to see many biological structures - usually requires contrast agents/staining to increase contrast
| Phase contrast / DIC | Makes use of slight changes in the ‘phase’ of light as it passes through a sample to increase contrast | Allows unstained samples to be seen more easily
| Widefield flourescence | Fluorescent labels (with specific excitation and emission wavelengths) bind to specific biological structures | Widefield illuminates the whole sample at once, which can lead to blurry images in thicker samples
| Widefield fluorescence | Fluorescent labels (with specific excitation and emission wavelengths) bind to specific biological structures | Widefield illuminates the whole sample at once, which can lead to blurry images in thicker samples
| Laser scanning confocal | A laser is scanned across the sample point by point in a raster pattern | Allows 'optical sectioning', giving clearer images in full 3D. More complex to use than widefield, also slower to acquire images.
| Spinning disc confocal | An array of pinholes on a disc split the laser into hundreds of beams that move across the sample | Faster than standard laser scanning confocal
| Super-resolution | Wide range of methods that break the classic 'diffraction limit' of light, allowing resolutions down to tens of nanometres | More complex to use than standard widefield / confocal
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2 changes: 1 addition & 1 deletion episodes/filetypes-and-metadata.md
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Expand Up @@ -237,7 +237,7 @@ During the [setup instructions](../learners/setup.md), we installed the

If you want to open more types of file, you will have to install the relevant
reader following the
[intructions in the BioIO docs](https://bioio-devs.github.io/bioio/OVERVIEW.html).
[instructions in the BioIO docs](https://bioio-devs.github.io/bioio/OVERVIEW.html).
Note the `bioio-bioformats` reader should allow a wide range of file formats to
be read.

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4 changes: 2 additions & 2 deletions episodes/imaging-software.md
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Expand Up @@ -86,7 +86,7 @@ Plugins, in contrast to scripts, are focused on adding optional new features to
a piece of software (rather than automating use of existing features). They
allow members of the community, outside the main team that develops the
software, to add features they need for a particular image type or processing
task. They're designed to be re-useable so other members of the community can
task. They're designed to be reusable so other members of the community can
easily benefit from these new features.

::::::::::::::::::::::::::::::::::::::::::::::::
Expand Down Expand Up @@ -254,7 +254,7 @@ menu-bar of Napari and select:

You should see a fluorescence microscopy image of some cells:

![](fig/cells-napari.png){alt="A screenshot of a flourescence microscopy image
![](fig/cells-napari.png){alt="A screenshot of a fluorescence microscopy image
of some cells in Napari"}

## Napari's User interface
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10 changes: 5 additions & 5 deletions episodes/instance-segmentation-and-measurements.md
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Expand Up @@ -8,7 +8,7 @@ exercises: 15

- How do we perform instance segmentation in Napari?
- How do we measure cell size with Napari?
- How do we save our work to create re-usable workflows?
- How do we save our work to create reusable workflows?

::::::::::::::::::::::::::::::::::::::::::::::::

Expand All @@ -20,7 +20,7 @@ exercises: 15

- Calculate the number of cells and average cell volume.

- Save and edit your workflow to re-use on subsequent images.
- Save and edit your workflow to reuse on subsequent images.

- Perform more complex cell shape analysis using scikit-image's `regionprops`.

Expand Down Expand Up @@ -365,7 +365,7 @@ again.
viewer.layers.remove('instance_seg')

eroded_semantic_seg = viewer.layers['eroded ball 10'].data
# Create a new instace segmentation using the eroded mask
# Create a new instance segmentation using the eroded mask
instance_seg = label(eroded_semantic_seg)

viewer.add_labels(instance_seg)
Expand Down Expand Up @@ -498,7 +498,7 @@ There are 11 individual nuclei

You now have a correct instance segmentation. You could return to
using the napari-skimage plugin to calculate the sizes
of each nucleus and export the results to a speadsheet or your preferred
of each nucleus and export the results to a spreadsheet or your preferred
analysis software using the `Save Results` function. However you've probably
picked up enough Python during this course to complete the analysis you need
with just the Napari console. Let's give it a try. The following commands
Expand Down Expand Up @@ -608,7 +608,7 @@ These numbers provide a good quantitative measure of the quantity and
volume of cell nuclei suitable for an experiment investigating how these
quantities change over time.

We can save our work from the console for re-use on data from subsequent
We can save our work from the console for reuse on data from subsequent
time points, creating a repeatable measurement workflow.

```python
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4 changes: 2 additions & 2 deletions episodes/multi-dimensional-images.md
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Expand Up @@ -156,7 +156,7 @@ Next, let's look at a 3D image where an additional (fourth) dimension contains
data from different 'channels'. Remove the brain image and select:
`File > Open Sample > napari builtins > Cells (3D+2Ch)`

![](fig/cells-napari.png){alt="A screenshot of a flourescence microscopy image
![](fig/cells-napari.png){alt="A screenshot of a fluorescence microscopy image
of some cells in Napari"}

Image channels can be used to store data from multiple sources for the same
Expand All @@ -178,7 +178,7 @@ This diagram shows an example of a 2D image with channels, but this can also be
extended to 3D images, as we will see now.

Fluorescence microscopy images, like the one we currently have open in Napari,
are common examples of images with multiple channels. In flourescence
are common examples of images with multiple channels. In fluorescence
microscopy, different 'flourophores' are used that target specific features
(like the nucleus or cell membrane) and emit light of different wavelengths.
Images are taken filtering for each of these wavelengths in turn, giving one
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4 changes: 2 additions & 2 deletions episodes/quality-control-and-manual-segmentation.md
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Expand Up @@ -29,7 +29,7 @@ number of cells in an image.
First, let's open one of Napari's sample images with:
`File > Open Sample > napari builtins > Cells (3D + 2Ch)`

![](fig/cells-napari.png){alt="A screenshot of a flourescence microscopy image
![](fig/cells-napari.png){alt="A screenshot of a fluorescence microscopy image
of some cells in Napari"}

## Quality control
Expand Down Expand Up @@ -623,7 +623,7 @@ These models learn to segment images based on provided 'groundtruth' data. This
interest. While generating this manual groundtruth is still slow and time
consuming, the advantage is that we only need to segment a small subset of our
images (rather than the entire thing). Also, once the model is trained, it can
be re-used on similar image data.
be reused on similar image data.

It's worth bearing in mind that automated methods are rarely perfect (whether
they're classic image processing, machine learning or deep learning based). It's
Expand Down