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<img src="https://github.com/Kalan-Lab/codoff/assets/4260723/05b6c7f1-9e8f-4865-b0fd-758c933bab83" width="300">
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> [!IMPORTANT]
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> **This is useful because it could indicate that the focal gene cluster has been horizontally transfered. While quick and easy to assess, we encourage users to further investigate if HGT is indeed responsible for any such signal. This is because a discordance between the codon usage for the focal gene cluster and background genome could be due to other reasons, e.g. infrequent expression of the focal gene cluster.**
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codoff is largely adapted from the `compareBGCtoGenomeCodonUsage.py` script in the [*lsa*BGC](https://github.com/Kalan-Lab/lsaBGC) suite and presented here separately just to make it easy to install via bioconda, therefore, please cite:
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> [Evolutionary investigations of the biosynthetic diversity in the skin microbiome using *lsa*BGC](https://www.microbiologyresearch.org/content/journal/mgen/10.1099/mgen.0.000988). *Microbial Genomics 2023.* Rauf Salamzade, J.Z. Alex Cheong, Shelby Sandstrom, Mary Hannah Swaney, Reed M. Stubbendieck, Nicole Lane Starr, Cameron R. Currie, Anne Marie Singh, and Lindsay R. Kalan
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If you have suggestions or feedback, please let us know in the GitHub issues.
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> **This is useful because it could indicate that the focal gene cluster has been horizontally transfered. While quick and easy to assess, we encourage users to further investigate if HGT is indeed responsible for any such signal. This is because a discordance between the codon usage for the focal gene cluster and background genome could be due to other reasons, e.g. infrequent/highly-conditional expression of the focal gene cluster.**
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## Installation
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OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.
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codoff is largely adapted from the `compareBGCtoGenomeCodonUsage.py` script in the [*lsa*BGC](https://github.com/Kalan-Lab/lsaBGC) suite and presented here separately just to make it easy to install via Bioconda.
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If you have suggestions or feedback, please let us know in the GitHub issues.
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## Acknowledgments
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codoff utilizes pyrodigal underneath to perform gene calling for bacterial genomes when requested:

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