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We present the three use case examples to demonstrate the application of YACHT for identifying taxonomy in microbiome studies: (i) analyzing low-abundance metagenomic samples that are common in clinical settings, (ii) performing MAG fishing to detect specific metagenomic-assembled genomes, and (iii) evaluating synthetic microbial communities to identify the presence of specific organisms.
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**Low abundance samples:** YACHT can analyze metagenomic samples with low microbial DNA concentrations common in clinical and environmental studies. Using a human skin metagenomics sample, we show that ANI threshold and k-size markedly influence species specificity. See [Low abundance samples](https://github.com/KoslickiLab/YACHT/tree/main/use_case_examples/low_abundance_samples).
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**Low abundance samples:** YACHT can analyze metagenomic samples with low microbial DNA concentrations common in clinical and environmental studies. Using a human skin metagenomics sample, we show that ANI threshold and $k$-size markedly influence species specificity. See [Low abundance samples](https://github.com/KoslickiLab/YACHT/tree/main/use_case_examples/low_abundance_samples).
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**Metagenomic-assembled genome (MAG) fishing:** Using a single MAG as a training reference database, YACHT searches for specific MAGs within a sample. Applied to two skin metagenomic samples, results shows that detection is sensitive to sequencing depth, coverage, and parameter choice. See [MAG fishing](https://github.com/KoslickiLab/YACHT/tree/main/use_case_examples/MAG_fishing).
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