Using ophys.ImagingPlane vs NWBFile.create_imaging_plane()

[jmdelahanty]

Hello everyone!

I was wondering about which of these two options I should be using when creating my NWB File for 2P experiments.

How should I best use create_imaging_plane and is there any difference between it and the ophys.ImagingPlane method? If I build an imaging plane with the NWBFile method, can I still append a TwoPhotonSeries to it when I have that ready?

Thanks for your help!

[bendichter]

NWBFile.create_imaging_plane creates an instance of an ImagingPlane and automatically adds it to the NWBFile object. I would suspect that is more convenient for you.

TwoPhotonSeries takes an imaging_plane argument, which links it to a given imaging plane. So all together, it's:

nwbfile = NWBFile(...)
...
imaging_plane = nwbfile.create_imaging_plane(...)
...
nwbfile.add_acquisition(TwoPhotonSeries(imaging_plane=imaging_plane, ...))

[jmdelahanty]

Perfect! That's just what I was looking for. Thanks for your help Ben!