eDNA Senior Design Updates

About the eDNA Senior Design Project

We are a group of students in the Ecological Engineering Senior Design (BEE 469 and 470) class. We are working on evaluating the performance of the OPEnS Lab eDNA Sampler (PolyWAG) for our senior design project. This evaluation will take place over fall and winter terms and will include the following components:

• Cross-contamination testing using a fluorometer and rhodamine dye
• DNA preservation testing using prepared DNA samples
• Construction of new samplers (and evaluation of associated documentation)
• Field testing

Our senior design team members are Lillian Peters, Jessica Chaplen, Kathleen Larson, and Jordan Laundry.

This page will be updated biweekly with links to memos detailing our progress.

---

Biweekly Memo 3: This memo includes our cross-contamination testing protocol, our dye concentration calculations, and an explanation of our preliminary decision matrix for field testing (the matrix itself is in a link below). We have also updated our timeline for cross-contamination testing.(Decision Matrix)

---

Biweekly Memo 2: In this memo we give information on the background research we have done, we make minor changes to our timeline for cross-contamination testing, and we list a few questions we have for Dr. Udell and Dr. Levi.

---

Biweekly Memo 1: In this memo we include our formal problem statement and plans for background research