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Reorder report subsections about temporal signal
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@@ -117,10 +117,10 @@ using [Freyja](https://github.com/andersen-lab/Freyja).
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![Estimated lineage admixture of each sample.
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Samples in the X-axis are ordered chronologically, from more ancient to newer.](`r params$freyja`){#fig-freyja}
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### Phylogeny and temporal signal
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### Phylogenetic reconstruction
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A maximum likelihood tree of the target and context samples has been
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build using [IQTREE](http://www.iqtree.org/).
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built using [IQTREE](http://www.iqtree.org/).
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The target samples `r stats[["monophyly"]]` monophyletic. The clade
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that contains all the target samples is supported by a **UFBoot** score of
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$`r stats[["boot"]]`$% and a **SH-aLRT** score of $`r stats[["alrt"]]`$% (@fig-tree_ml).
@@ -129,22 +129,6 @@ $`r stats[["boot"]]`$% and a **SH-aLRT** score of $`r stats[["alrt"]]`$% (@fig-t
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target datasets and their context samples. The clade that contains the target
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samples is squared in red.](`r params$tree_ml`){#fig-tree_ml}
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A neighbor-joining tree has been constructed using pairwise distances
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between target samples (@fig-tree), based on the allele frequencies measured from
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read mappings.
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![Neighbor-joining tree based on the pairwise allele
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frequency-weighted distances.](`r params$tree`){#fig-tree}
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Root-to-tip distances measured on this tree have been correlated
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with time, obtaining a $R^2$ of **`r correlation`** and a p-value of
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$`r p_value_lm`$. The estimated substitution rate is **`r sub_rate`**
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substitutions per year (@fig-tempest).
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![Scatterplot depicting the relationship between root-to-tip
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distances and the number of days passed since the first sample. The red
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line shows the linear model fit.](`r params$tempest`){#fig-tempest}
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### Nucleotide diversity comparison
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Nucleotide diversity (π) has been calculated for $`r div_values[["boot.reps"]]`$ random
@@ -206,7 +190,7 @@ at the bottom, and the latest, at the top.](`r params$SNV_s`){#fig-SNV_s}
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:::
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### Time dependency for the intra-host nucleotide variants
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### Temporal signal of the intra-host mutations
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The correlation of the allele frequency of each NV with the time since the
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initial sampling has been calculated (@fig-volcano).
@@ -223,6 +207,22 @@ nucleotide variants with a significant correlation with time and sites with more
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than two possible states. Each subplot depicts the progression of the allele
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frequencies in time for a given genome position.](`r params$panel`){#fig-panel}
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A neighbor-joining tree has been constructed using pairwise distances
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between target samples (@fig-tree), based on the allele frequencies measured from
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read mappings.
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![Neighbor-joining tree based on the pairwise allele
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frequency-weighted distances.](`r params$tree`){#fig-tree}
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To estimate the substitution rate, root-to-tip distances measured on the previous
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tree (@fig-tree) have been correlated with time, obtaining a $R^2$ of
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**`r correlation`** and a p-value of $`r p_value_lm`$. The estimated substitution
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rate is **`r sub_rate`** substitutions per year (@fig-tempest).
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![Scatterplot depicting the relationship between root-to-tip
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distances and the number of days passed since the first sample. The red
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line shows the linear model fit.](`r params$tempest`){#fig-tempest}
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### Correlation between alternative alleles
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To detect possible interactions between mutations, pairwise correlation between allele

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