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developer-guide/05-Scripts/01-writing-scripts.md

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```
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You can also use the [Pioreactor's plugin architecture](https://docs.pioreactor.com/developer-guide/intro-plugins#scripts) to control the start and stop of the script.
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You can also use the [Pioreactor's plugin architecture](https://docs.pioreactor.com/developer-guide/intro-plugins#custom-scripts) to control the start and stop of the script.
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### Useful utility objects
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developer-guide/07-Plugins/02-adding-plugins-to-ui.md

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published_settings: []
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```
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See more information on the structure of your script [here](/developer-guide/intro-plugins#scripts)
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See more information on the structure of your script [here](/developer-guide/intro-plugins#custom-scripts)
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## Automations
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developer-guide/09-Storage and the filesystem/01-data-stores/02-sqlite.md

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### How can I add new SQL tables?
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Using the sqlite shell (`pio db`), you can execute any SQL statements, including creating new tables. Plugins can also automatically create new tables when installed, [see instructions here](plugin-as-python-package#5-optional-adding-tables-to-the-sql-store).
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Using the sqlite shell (`pio db`), you can execute any SQL statements, including creating new tables. Plugins can also automatically create new tables when installed, [see instructions here](plugin-as-python-package#5-optional-adding-tables-to-the-sql-database-and-exposing-them-on-the-export-data-page).
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### How do I populate my own SQL tables?
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experiments/02-Secondary level education/caffeine-inhibition.md

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1. Prepare identical sterile stocks in vials with your media of choice.
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2. Add some amount (a few drops to 1 mL) of a liquid containing caffeine to a vial. Add the same amount of DI water to another vial.
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* If the media appears different before inoculation, we recommend you [blank](/user-guide/set-up-an-experiment#blanking) your vials before adding your culture.
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* If the media appears different before inoculation, we recommend you [blank](/user-guide/od-normal-growth-rate#blanking) your vials before adding your culture.
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3. Inoculate each vial with a very small amount of your microorganism, using best practices to avoid other contamination.
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* In our example, a yeast stock solution can be made by diluting a small amount of yeast in 15mL of YPD stock media, then a drop of this stock solution can be added to your vials.
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4. Wipe the vials and place them in the Pioreactors.

experiments/02-Secondary level education/low-vs-high-conc-media.md

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## Detailed procedure
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1. Prepare a sterile stock of two different concentrations of your media of choice.
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* If the media appears different before inoculation, we recommend you [blank](/user-guide/set-up-an-experiment#blanking) your vials before adding your culture.
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* If the media appears different before inoculation, we recommend you [blank](/user-guide/od-normal-growth-rate#blanking) your vials before adding your culture.
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2. Inoculate each vial with a very small amount of your microorganism, using best practices to avoid other contamination.
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* In our example, a yeast stock solution can be made by diluting a small amount of yeast in 15mL of YPD stock media, then a drop of this stock solution can be added to your vials.
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3. Wipe the vials and place them in the Pioreactors.

experiments/03-University level education/00-continuous-cultures-using-turbidostat.md

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## Introduction
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Your Pioreactor can be transformed into a [turbidostat](/user-guide/dosing-automations#pid-turbidostat), where pumps are used to replenish media around a target OD, creating a continuous culture. Due to the abundance of resources and lack of rate-limiting nutrients, the specific growth rate of a species is maximized. _Learn more about the differences in bioreactor strategies [here](https://pioreactor.com/blogs/pioreactor/the-many-different-environments-of-bioreactors-chemostat-turbidostat-stressostat-and-more)!_
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Your Pioreactor can be transformed into a [turbidostat](/user-guide/dosing-automations#turbidostat), where pumps are used to replenish media around a target OD, creating a continuous culture. Due to the abundance of resources and lack of rate-limiting nutrients, the specific growth rate of a species is maximized. _Learn more about the differences in bioreactor strategies [here](https://pioreactor.com/blogs/pioreactor/the-many-different-environments-of-bioreactors-chemostat-turbidostat-stressostat-and-more)!_
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This setup is a powerful tool that tests the limits of population growth. Students can investigate the specific maximum growth rate of a species and evaluate what innate biological processes are limiting growth.
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user-guide/01-getting-started/01-assembly_guides/00_pioreactor_40ml_v1.5/02-40ml-v15-rpi-hat-assembly.md

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<h2 id="raspberry-pi-a-or-b-instructions">Raspberry Pi A or B instructions</h2>
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## Raspberry Pi A or B instructions {#raspberry-pi-a-or-b-instructions}
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<AssemblyInstructionBlock title="Step 2: Assemble the bottom faceplate" images={["user-guide/hardware-assembly/upgrade-version/4B-10mm-screws.png","user-guide/hardware-assembly/upgrade-version/4B-hexnuts.png","user-guide/hardware-assembly/upgrade-version/4B-gpios.png"]}>
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</AssemblyInstructionBlock>
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<h2 id="raspberry-pi-zero-zero-2-instructions">Raspberry Pi Zero / Zero 2 instructions</h2>
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## Raspberry Pi Zero / Zero 2 instructions {#raspberry-pi-zero-zero-2-instructions}
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<AssemblyInstructionBlock title="Step 2: Assemble the bottom faceplate" images={["user-guide/hardware-assembly/upgrade-version/zero-10mm-screws.png","user-guide/hardware-assembly/upgrade-version/zero-hexnuts.png","user-guide/hardware-assembly/upgrade-version/zero-level-check.png","user-guide/hardware-assembly/upgrade-version/zero-gpios.png"]}>
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user-guide/01-getting-started/01-assembly_guides/01_pioreactor_20ml_v1.5/02-20ml-v15-rpi-hat-assembly.md

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<h2 id="raspberry-pi-a-or-b-instructions">Raspberry Pi A or B instructions</h2>
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## Raspberry Pi A or B instructions {#raspberry-pi-a-or-b-instructions}
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<AssemblyInstructionBlock title="Step 2: Assemble the bottom faceplate" images={["user-guide/hardware-assembly/upgrade-version/4B-10mm-screws.png","user-guide/hardware-assembly/upgrade-version/4B-hexnuts.png","user-guide/hardware-assembly/upgrade-version/4B-gpios.png"]}>
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</AssemblyInstructionBlock>
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<h2 id="raspberry-pi-zero-zero-2-instructions">Raspberry Pi Zero / Zero 2 instructions</h2>
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## Raspberry Pi Zero / Zero 2 instructions {#raspberry-pi-zero-zero-2-instructions}
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<AssemblyInstructionBlock title="Step 2: Assemble the bottom faceplate" images={["user-guide/hardware-assembly/upgrade-version/zero-10mm-screws.png","user-guide/hardware-assembly/upgrade-version/zero-hexnuts.png","user-guide/hardware-assembly/upgrade-version/zero-level-check.png","user-guide/hardware-assembly/upgrade-version/zero-gpios.png"]}>
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user-guide/01-getting-started/01-assembly_guides/90_pioreactor_40ml/02-40ml-rpi-hat-assembly.md

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<h2 id="raspberry-pi-a-or-b-instructions">Raspberry Pi A or B instructions</h2>
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## Raspberry Pi A or B instructions {#raspberry-pi-a-or-b-instructions}
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<AssemblyInstructionBlock title="Step 2: Assemble the bottom faceplate" images={["user-guide/hardware-assembly/upgrade-version/4B-10mm-screws.png","user-guide/hardware-assembly/upgrade-version/4B-hexnuts.png","user-guide/hardware-assembly/upgrade-version/4B-gpios.png"]}>
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</AssemblyInstructionBlock>
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<h2 id="raspberry-pi-zero-zero-2-instructions">Raspberry Pi Zero / Zero 2 instructions</h2>
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## Raspberry Pi Zero / Zero 2 instructions {#raspberry-pi-zero-zero-2-instructions}
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<AssemblyInstructionBlock title="Step 2: Assemble the bottom faceplate" images={["user-guide/hardware-assembly/upgrade-version/zero-10mm-screws.png","user-guide/hardware-assembly/upgrade-version/zero-hexnuts.png","user-guide/hardware-assembly/upgrade-version/zero-level-check.png","user-guide/hardware-assembly/upgrade-version/zero-gpios.png"]}>
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user-guide/01-getting-started/01-assembly_guides/91_pioreactor_20ml_v1.1/02-v11-rpi-hat-assembly.md

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<h2 id="raspberry-pi-a-or-b-instructions">Raspberry Pi A or B instructions</h2>
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## Raspberry Pi A or B instructions {#raspberry-pi-a-or-b-instructions}
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<AssemblyInstructionBlock title="Step 2: Assemble the bottom faceplate" images={["user-guide/hardware-assembly/upgrade-version/4B-10mm-screws.png","user-guide/hardware-assembly/upgrade-version/4B-hexnuts.png","user-guide/hardware-assembly/upgrade-version/4B-gpios.png"]}>
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</AssemblyInstructionBlock>
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<h2 id="raspberry-pi-zero-zero-2-instructions">Raspberry Pi Zero / Zero 2 instructions</h2>
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## Raspberry Pi Zero / Zero 2 instructions {#raspberry-pi-zero-zero-2-instructions}
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<AssemblyInstructionBlock title="Step 2: Assemble the bottom faceplate" images={["user-guide/hardware-assembly/upgrade-version/zero-10mm-screws.png","user-guide/hardware-assembly/upgrade-version/zero-hexnuts.png","user-guide/hardware-assembly/upgrade-version/zero-level-check.png","user-guide/hardware-assembly/upgrade-version/zero-gpios.png"]}>
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