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Copy file name to clipboardExpand all lines: experiments/02-Secondary level education/yeast-in-ethanol.md
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@@ -44,17 +44,17 @@ We conducted an experiment using 4 Pioreactors and vials with ethanol concentrat
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The first concentration of 0% ethanol is easy — we just add 1 mL of distilled water. For the rest, the following calculations were done to determine the volume of ethanol to add to each vial:
In our case, we have **15 mL** of YPD broth per vial, and want to add **1 mL** of diluted ethanol; we want to determine our final concentration of ethanol **in 1 mL** to get an overall 2% concentration in our complete, 16 mL of media.
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For 2%:
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$$
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\frac\{[\text\{ethanol\}]\}\{\text\{15 mL + 1 mL\}\}=\text\{2\%\}
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\frac{[\text{ethanol}]}{\text{15 mL + 1 mL}}=\text{2\%}
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$$
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$$
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[\text\{ethanol\}]=32\%
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[\text{ethanol}] = 32\%
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$$
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Now, we know we want our 1 mL of dilute ethanol concentration to equal 32%. Since we're starting with an initial concentration of 95%, we can apply the following dilution equation to figure out the concentration of 95% ethanol to use:
@@ -67,10 +67,10 @@ Final concentration (C<sub>f</sub>)= 32%
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Now we substitute our variables into the equation C<sub>i</sub> V<sub>i</sub> = C<sub>f</sub> V<sub>f</sub>:
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