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developer-guide/20-User interface/06-unit-api-reference.md

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@@ -673,8 +673,6 @@ Get Running Jobs For Experiment endpoint.
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## Get Job Settings
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{
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### Endpoint
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`GET /unit_api/jobs/settings/job_name/{job_name}`
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experiments/02-Secondary level education/low-vs-high-conc-media.md

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@@ -40,14 +40,14 @@ Discuss with your students the following ideas:
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We created 1% YPD by adding 0.15 grams of YPD broth powder to 15 mL:
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$$\text \{w/v \%\}= \frac \{\text\{mass\}\}\{\text \{volume\}\}$$
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$$\text{w/v \%} = \frac{\text{mass}}{\text{volume}}$$
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$$
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\text \{mass\} = \{0.01\} \times \{15 \:\text \{mL\}\}
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\text{mass} = 0.01 \times 15 \:\text{mL}
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$$
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$$
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\text \{mass\} = 0.15 \:\text \{grams\}
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\text{mass} = 0.15 \:\text{grams}
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$$
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Using the same logic, 5% YPD was created by adding 0.75 grams to 15 mL.

experiments/02-Secondary level education/yeast-in-ethanol.md

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@@ -44,17 +44,17 @@ We conducted an experiment using 4 Pioreactors and vials with ethanol concentrat
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The first concentration of 0% ethanol is easy &#151 we just add 1 mL of distilled water. For the rest, the following calculations were done to determine the volume of ethanol to add to each vial:
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$$
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\frac\{[\text\{ethanol\}]\}\{\text\{media\thickspace volume + ethanol\thickspace volume\}\}=\text\{ethanol \%\}
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\frac{[\text{ethanol}]}{\text{media volume + ethanol volume}}=\text{ethanol \%}
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$$
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In our case, we have **15 mL** of YPD broth per vial, and want to add **1 mL** of diluted ethanol; we want to determine our final concentration of ethanol **in 1 mL** to get an overall 2% concentration in our complete, 16 mL of media.
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For 2%:
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$$
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\frac\{[\text\{ethanol\}]\}\{\text\{15 mL + 1 mL\}\}=\text\{2\%\}
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\frac{[\text{ethanol}]}{\text{15 mL + 1 mL}}=\text{2\%}
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$$
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$$
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[\text\{ethanol\}]=32\%
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[\text{ethanol}] = 32\%
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$$
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Now, we know we want our 1 mL of dilute ethanol concentration to equal 32%. Since we're starting with an initial concentration of 95%, we can apply the following dilution equation to figure out the concentration of 95% ethanol to use:
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Now we substitute our variables into the equation C<sub>i</sub> V<sub>i</sub> = C<sub>f</sub> V<sub>f</sub>:
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$$
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V_i = \frac\{(32\%)(\text\{1 mL\})\}\{95\%\}
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V_i = \frac{(32\%)(\text{1 mL})}{95\%}
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$$
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$$
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V_i = \text\{0.34 mL\}
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V_i = \text{0.34 mL}
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$$
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So the composition to create 2% ethanol media is:
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To calculate the resulting ethanol percentage:
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$$
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\frac\{[\text\{ethanol\}]\}\{\text\{media\thickspace volume + ethanol\thickspace volume\}\}=\text\{ethanol \%\}
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\frac{[\text{ethanol}]}{\text{media volume + ethanol volume}}=\text{ethanol \%}
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$$
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Substitute the [ethanol] value for half of 95% (or 47.5%):
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$$
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\frac\{47.5\%\}\{\text\{15 mL + 1 mL\}\} = \text\{ethanol \%\}
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\frac{47.5\%}{\text{15 mL + 1 mL}} = \text{ethanol \%}
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$$
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$$
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\text\{ethanol \%\} \approx 3\%
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\text{ethanol \%} \approx 3\%
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$$
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This technique produces halving ethanol percentages of 0%, 1.5%, 3%, and 6% to be studied on a cluster of 4 Pioreactors.
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Alba-Lois, L., & Segal-Kischinevzky, C. (2010). Yeast fermentation and the making of beer and wine. _Nature Education, 3_(9), 17.
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Gray, W. D. (1941). Studies on the alcohol tolerance of yeasts. _Journal of Bacteriology, 42_(5), 561-574.
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Gray, W. D. (1941). Studies on the alcohol tolerance of yeasts. _Journal of Bacteriology, 42_(5), 561-574.

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