diff --git a/.murdock b/.murdock
index 416a7cffbfbf..711dfd3e62fe 100755
--- a/.murdock
+++ b/.murdock
@@ -52,6 +52,7 @@ export CFLAGS_DBG=""
 export DLCACHE_DIR=${DLCACHE_DIR:-~/.dlcache}
 export ENABLE_TEST_CACHE=${ENABLE_TEST_CACHE:-1}
 export MURDOCK_REDIS_HOST=${MURDOCK_REDIS_HOST:-127.0.0.1}
+export PYTHON3_WITH_PEP723=python3
 
 NIGHTLY=${NIGHTLY:-0}
 
diff --git a/CODING_CONVENTIONS.md b/CODING_CONVENTIONS.md
index c3c414b77534..76e5d37e36b3 100644
--- a/CODING_CONVENTIONS.md
+++ b/CODING_CONVENTIONS.md
@@ -701,15 +701,32 @@ not a string literal`.
       [McCabe project](https://pypi.python.org/pypi/mccabe)
 * A line length of maximum of 120 is allowed instead of 79 as per PEP 8. This
   increases tests readability as they can expects long line of output.
-* Only runnable scripts shall start with `#!/usr/bin/env python3`
+* All runnable scripts and only runnable scripts **MUST** have a shebang
+* Runnable scripts **SHOULD** use `#!/usr/bin/env -S uv run` as shebang
+* [PEP 723 Inline script metadata](https://peps.python.org/pep-0723/) **SHOULD**
+  be used for runnable scripts to declare dependencies
+* Calls to the python executables from the build system **MUST** be done with
+  the wrapper `PYTHON3_WITH_PEP723` if they have PEP 723 annotations.
+    * E.g. to run `dist/tools/fancy/fancy.py` use the following snippet:
+      `$(Q)$(PYTHON3_WITH_PEP723) $(RIOTTOOLS)/fancy/fancy.py`
+    * In the CI, `PYTHON3_WITH_PEP723` is overwritten as `python3` and the
+      CI container **MUST** ship all required python modules to avoid duplicate
+      downloads and slower CI time due to venv setup time
 * Runnable scripts shall use the following scheme:
 
 ```python
-#!/usr/bin/env python3
+#!/usr/bin/env -S uv run
 
 # SPDX-FileCopyrightText: 2026 <your name/company>
 # SPDX-License-Identifier: LGPL-2.1-only
 
+# /// script
+# requires-python = ">=3.10"
+# dependencies = [
+#   "pyudev"
+# ]
+# ///
+
 # put the module imports first
 # see https://www.python.org/dev/peps/pep-0008/#imports
 # for more details
diff --git a/Makefile.include b/Makefile.include
index 75f70b77a4c3..ad2c58fc9b57 100644
--- a/Makefile.include
+++ b/Makefile.include
@@ -186,6 +186,12 @@ OS_ARCH = $(word 2, $(UNAME))
 # set python path, e.g. for tests
 PYTHONPATH := $(RIOTBASE)/dist/pythonlibs/:$(PYTHONPATH)
 
+# Set python3 frontend capable of PEP 723 inline script metadata. Users may
+# choose `pipx run` or just `python3` instead. In case of just `python3`, there
+# will be no PEP 723 support and users will just have to install the right
+# python modules beforehand.
+PYTHON3_WITH_PEP723 ?= uv run
+
 # Basic utilities included before anything else
 include $(RIOTMAKE)/utils/checks.mk
 
@@ -877,7 +883,7 @@ cleanterm: $(TERMDEPS)
 	$(TERMPROG) $(TERMFLAGS) $(TERMTEE)
 
 list-ttys:
-	$(Q)$(RIOTTOOLS)/usb-serial/ttys.py
+	$(Q)$(PYTHON3_WITH_PEP723) $(RIOTTOOLS)/usb-serial/ttys.py
 
 doc doc-man doc-latex:
 	$(MAKE) -C $(RIOTBASE) $@
diff --git a/dist/tools/usb-serial/ttys.py b/dist/tools/usb-serial/ttys.py
index 38b6ed3415ac..402337cd5715 100755
--- a/dist/tools/usb-serial/ttys.py
+++ b/dist/tools/usb-serial/ttys.py
@@ -1,4 +1,15 @@
-#!/usr/bin/env python3
+#!/usr/bin/env -S uv run
+
+# SPDX-FileCopyrightText: 2023 Otto-von-Guericke-Universität Magdeburg
+# SPDX-FileCopyrightText: 2026 ML!PA Consulting GmbH
+# SPDX-License-Identifier: LGPL-2.1-only
+
+# /// script
+# requires-python = ">=3.10"
+# dependencies = [
+#   "pyudev"
+# ]
+# ///
 """
 Command line utility to list and filter TTYs
 """