I am trying to recreate the FRETraj-demo results locally without success. I cloned the FRETraj-demo repo and installed FRETraj in a clean python 3.8 environment with conda. I then installed the necessary dependencies as needed.
Here's a text file for the current environment: environment.txt.
I ran the original notebook on Binder (no changes made) and compared the results to my local instance to try to see where things went wrong. At the calculation of the ACVs my local results differ. Running
acv_D = ft.cloud.Volume.from_frames(traj, 'Cy3-20-C5', labels, selected_frames)
acv_A = ft.cloud.Volume.from_frames(traj, 'Cy5-44-P1', labels, selected_frames)
results in the ValueError messages Empty Accessible volume at position 626. Is your attachment point buried? and Empty Accessible volume at position 1395. Is your attachment point buried?.
Any ideas as to how I can resolve this discrepancy?
I am trying to recreate the FRETraj-demo results locally without success. I cloned the FRETraj-demo repo and installed FRETraj in a clean python 3.8 environment with conda. I then installed the necessary dependencies as needed.
Here's a text file for the current environment: environment.txt.
I ran the original notebook on Binder (no changes made) and compared the results to my local instance to try to see where things went wrong. At the calculation of the ACVs my local results differ. Running
results in the ValueError messages
Empty Accessible volume at position 626. Is your attachment point buried?andEmpty Accessible volume at position 1395. Is your attachment point buried?.Any ideas as to how I can resolve this discrepancy?