diff --git a/.github/workflows/mypy-type-check.yml b/.github/workflows/mypy-type-check.yml
index 7cba5efe0..a2aecec3b 100644
--- a/.github/workflows/mypy-type-check.yml
+++ b/.github/workflows/mypy-type-check.yml
@@ -18,7 +18,7 @@ jobs:
 
     strategy:
       matrix:
-        python-version: ["3.11", "3.12", "3.13"]
+        python-version: ["3.11", "3.12", "3.13", "3.14"]
 
     steps:
 
diff --git a/.github/workflows/pip-install.yml b/.github/workflows/pip-install.yml
index 515ce35de..4ac16c06a 100644
--- a/.github/workflows/pip-install.yml
+++ b/.github/workflows/pip-install.yml
@@ -31,7 +31,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        python-version: ["3.11", "3.12", "3.13"]
+        python-version: ["3.11", "3.12", "3.13", "3.14"]
         os: [ubuntu-24.04, windows-latest, macos-latest]
 
     # Force UTF-8 everywhere (Windows is the one that really needs it)
diff --git a/.github/workflows/python-package.yml b/.github/workflows/python-package.yml
index d7df43b29..c3948add1 100644
--- a/.github/workflows/python-package.yml
+++ b/.github/workflows/python-package.yml
@@ -20,7 +20,7 @@ jobs:
     strategy:
       fail-fast: true
       matrix:
-        python-version: ["3.11", "3.12", "3.13"]
+        python-version: ["3.11", "3.12", "3.13", "3.14"]
 
     steps:
     - uses: actions/checkout@v4
diff --git a/docs/installation.rst b/docs/installation.rst
index 41d85e232..340afb5f8 100644
--- a/docs/installation.rst
+++ b/docs/installation.rst
@@ -59,7 +59,7 @@ MacPorts
 Installing Stable Release
 =========================
 
-Please note that TIAToolbox is currently tested with Python versions 3.11, 3.12, and 3.13. For the full range of supported Python versions, please refer to the package metadata (for example, the ``python_requires`` setting in ``setup.py`` or ``pyproject.toml``)
+Please note that TIAToolbox is tested for Python versions 3.11, 3.12, 3.13 and 3.14. For the full range of supported Python versions, please refer to the package metadata (for example, the ``python_requires`` setting in ``setup.py`` or ``pyproject.toml``)
 
 Recommended
 -----------
diff --git a/requirements/requirements.conda.yml b/requirements/requirements.conda.yml
index e1160bf51..b1b3e4d99 100644
--- a/requirements/requirements.conda.yml
+++ b/requirements/requirements.conda.yml
@@ -8,6 +8,6 @@ dependencies:
   - cython
   - pip>=20.0.2
   - pixman>=0.39.0
-  - python>=3.11, <=3.13
+  - python>=3.11, <=3.14
   - pip:
     - -r requirements.txt
diff --git a/requirements/requirements.dev.conda.yml b/requirements/requirements.dev.conda.yml
index fd8162d5b..6c6188b83 100644
--- a/requirements/requirements.dev.conda.yml
+++ b/requirements/requirements.dev.conda.yml
@@ -8,6 +8,6 @@ dependencies:
   - cython
   - pip>=20.0.2
   - pixman>=0.39.0
-  - python>=3.11, <=3.13
+  - python>=3.11, <=3.14
   - pip:
       - -r requirements_dev.txt
diff --git a/requirements/requirements.txt b/requirements/requirements.txt
index e96eb5868..29b910255 100644
--- a/requirements/requirements.txt
+++ b/requirements/requirements.txt
@@ -30,7 +30,7 @@ scipy>=1.8
 shapely>=2.0.0
 SimpleITK>=2.2.1
 sphinx>=5.3.0, <9.0.0
-tifffile>=2022.10.10, <=2025.5.10
+tifffile>=2025.5.21
 timm>=1.0.3
 torch>=2.5.0
 torchvision>=0.15.0
@@ -38,4 +38,4 @@ tqdm>=4.64.1
 transformers>=4.51.1
 umap-learn>=0.5.3
 wsidicom>=0.18.0
-zarr>=2.13.3, <3.0.0
+zarr>=3.0.8
diff --git a/requirements/requirements.win64.conda.yml b/requirements/requirements.win64.conda.yml
index 3bf2d9484..fb84ea073 100644
--- a/requirements/requirements.win64.conda.yml
+++ b/requirements/requirements.win64.conda.yml
@@ -9,6 +9,6 @@ dependencies:
   - openjpeg>=2.4.0
   - pip>=20.0.2
   - pixman>=0.39.0
-  - python>=3.11, <=3.13
+  - python>=3.11, <=3.14
   - pip:
       - -r requirements.txt
diff --git a/requirements/requirements.win64.dev.conda.yml b/requirements/requirements.win64.dev.conda.yml
index 48999206a..bc7f34d85 100644
--- a/requirements/requirements.win64.dev.conda.yml
+++ b/requirements/requirements.win64.dev.conda.yml
@@ -9,6 +9,6 @@ dependencies:
   - openjpeg>=2.4.0
   - pip>=20.0.2
   - pixman>=0.39.0
-  - python>=3.11, <=3.13
+  - python>=3.11, <=3.14
   - pip:
       - -r requirements_dev.txt
diff --git a/setup.py b/setup.py
index 8f2302e3f..4736707cb 100644
--- a/setup.py
+++ b/setup.py
@@ -34,7 +34,7 @@
 setup(
     author="TIA Centre",
     author_email="TIA@warwick.ac.uk",
-    python_requires=">=3.11, <3.14",
+    python_requires=">=3.11, <3.15",
     classifiers=[
         "Development Status :: 2 - Pre-Alpha",
         "Intended Audience :: Developers",
@@ -43,6 +43,7 @@
         "Programming Language :: Python :: 3.11",
         "Programming Language :: Python :: 3.12",
         "Programming Language :: Python :: 3.13",
+        "Programming Language :: Python :: 3.14",
     ],
     description="Computational pathology toolbox developed by TIA Centre.",
     dependency_links=dependency_links,