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diff --git a/Manuals/RNAseq_Mapping_Galaxy_part2.pdf b/Manuals/RNAseq_Mapping_Galaxy_part2.pdf
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diff --git a/README.md b/README.md
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--- a/README.md
+++ b/README.md
@@ -32,11 +32,13 @@ Learn how to use online resources for genomic-scale data analysis of protozoan p
  
  Day 1:
  
- - [Basic searches in VEuPathDB – Site search](https://github.com/WCSCourses/Protozoan_Parasite_Database_Resources_2022/blob/main/Manuals/1_SiteSearch_PlasmoDB.pdf)
+ - Basic searches in VEuPathDB:
 
-- [Basic searches in VEuPathDB – Search strategies](https://github.com/WCSCourses/Protozoan_Parasite_Database_Resources_2022/blob/main/Manuals/1b_SearchStrategies_Exercise_PlasmoDB.pdf)
+   - [Basic searches in VEuPathDB – Site search](https://github.com/WCSCourses/Protozoan_Parasite_Database_Resources_2022/blob/main/Manuals/1_SiteSearch_PlasmoDB.pdf)
 
-- [Basic searches in VEuPathDB – Organism Preferences (optional exercise)](https://github.com/WCSCourses/Protozoan_Parasite_Database_Resources_2022/blob/main/Manuals/1c_Organism_Preferences.pdf)
+   - [Basic searches in VEuPathDB – Search strategies](https://github.com/WCSCourses/Protozoan_Parasite_Database_Resources_2022/blob/main/Manuals/1b_SearchStrategies_Exercise_PlasmoDB.pdf)
+
+   - [Basic searches in VEuPathDB – Organism Preferences (optional exercise)](https://github.com/WCSCourses/Protozoan_Parasite_Database_Resources_2022/blob/main/Manuals/1c_Organism_Preferences.pdf)
 
  - [The gene page in VEuPathDB](https://github.com/WCSCourses/Protozoan_Parasite_Database_Resources_2022/blob/main/Manuals/2_Exploring_the_Gene_Page.pdf)
  
@@ -63,14 +65,16 @@ Learn how to use online resources for genomic-scale data analysis of protozoan p
  Day 3: 
  
  - Intro to RNA Seq Analysis - will be a talk - slides will come.
+ - Group exercise: Starting and RNA Seq analysis workflow in Galaxy
+   - [Groups](https://github.com/WCSCourses/Protozoan_Parasite_Database_Resources_2022/blob/main/Manuals/RNAseq%20groups.pdf)
+   - [RNA Seq in Galaxy part 1](https://github.com/WCSCourses/Protozoan_Parasite_Database_Resources_2022/blob/main/Manuals/RNAseq_Mapping_Galaxy_part1.pdf)
  
  - [Transcriptomics/proteomic searches in VEuPathDB](https://github.com/WCSCourses/Protozoan_Parasite_Database_Resources_2022/blob/main/Manuals/6b_Proteomics.pdf)
  
  - [Orthology](https://github.com/WCSCourses/Protozoan_Parasite_Database_Resources_2022/blob/main/Manuals/7_Orthology_Phyletic_Patterns.pdf)
  
  Day 4:
- 
-- GO enrichment analysis 
+- [Exploring RNA Seq results from Galaxy part 2, including analysis of results in VEuPathDB](https://github.com/WCSCourses/Protozoan_Parasite_Database_Resources_2022/blob/main/Manuals/RNAseq_Mapping_Galaxy_part2.pdf)
  
 - Host response