diff --git a/packages/amplify-e2e-tests/functions/titlecase.pipfile b/packages/amplify-e2e-tests/functions/titlecase.pipfile
index 006096690e5..378ae445d57 100644
--- a/packages/amplify-e2e-tests/functions/titlecase.pipfile
+++ b/packages/amplify-e2e-tests/functions/titlecase.pipfile
@@ -9,4 +9,4 @@ verify_ssl = true
 titlecase = "==0.12.0"
 
 [requires]
-python_version = "3.8"
+python_version = "3.13"
diff --git a/packages/amplify-e2e-tests/src/__tests__/layer-2.test.ts b/packages/amplify-e2e-tests/src/__tests__/layer-2.test.ts
index a6007b19ebb..25ac819d747 100644
--- a/packages/amplify-e2e-tests/src/__tests__/layer-2.test.ts
+++ b/packages/amplify-e2e-tests/src/__tests__/layer-2.test.ts
@@ -371,13 +371,13 @@ describe('amplify add lambda layer with changes', () => {
     add python layer
     add files in opt
     push
-    remove lib/python3.8/site-packages (simulate gitignore),
+    remove lib/python3.13/site-packages (simulate gitignore),
     amplify status -> no change
     delete Pipfile.lock
     amplify status -> update
     push
     -> should not create layer version, (it should force a pip install),
-    lib/python3.8/site-packages should exist with content, push should succeed
+    lib/python3.13/site-packages should exist with content, push should succeed
   */
 
   it('add python layer, remove lock file, site-packages, verify status, push', async () => {
@@ -405,7 +405,7 @@ describe('amplify add lambda layer with changes', () => {
 
     const firstArn = getCurrentLayerArnFromMeta(projRoot, { layerName, projName });
 
-    // 1. Remove lib/python3.8/site-packages
+    // 1. Remove lib/python3.13/site-packages
     // 2. Check status: No Change
     const layerPath = path.join(
       projRoot,
diff --git a/packages/amplify-python-function-runtime-provider/resources/Pipfile b/packages/amplify-python-function-runtime-provider/resources/Pipfile
index b5846df18ca..4f819af807e 100644
--- a/packages/amplify-python-function-runtime-provider/resources/Pipfile
+++ b/packages/amplify-python-function-runtime-provider/resources/Pipfile
@@ -8,4 +8,4 @@ verify_ssl = true
 [packages]
 
 [requires]
-python_version = "3.8"
+python_version = "3.13"
diff --git a/packages/amplify-python-function-runtime-provider/src/index.ts b/packages/amplify-python-function-runtime-provider/src/index.ts
index 1dcab233868..39aa43cb384 100644
--- a/packages/amplify-python-function-runtime-provider/src/index.ts
+++ b/packages/amplify-python-function-runtime-provider/src/index.ts
@@ -18,7 +18,7 @@ export const functionRuntimeContributorFactory: FunctionRuntimeContributorFactor
         runtime: {
           name: 'Python',
           value: 'python',
-          cloudTemplateValue: 'python3.8',
+          cloudTemplateValue: 'python3.13',
           defaultHandler: 'index.handler',
           layerExecutablePath: 'python',
           layerDefaultFiles: [
diff --git a/packages/amplify-python-function-template-provider/resources/hello-world/Pipfile b/packages/amplify-python-function-template-provider/resources/hello-world/Pipfile
index 88b01f8a1d1..ee8e3838b95 100644
--- a/packages/amplify-python-function-template-provider/resources/hello-world/Pipfile
+++ b/packages/amplify-python-function-template-provider/resources/hello-world/Pipfile
@@ -9,4 +9,4 @@ verify_ssl = true
 src = {editable = true, path = "./src"}
 
 [requires]
-python_version = "3.8"
+python_version = "3.13"