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update requirements and README
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examples/custom-code-AD/README.md

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# Custom Code in Automation Designer
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## 5-parameter-logistic
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Example snippet fits a 5-Parameter Logistic (5PL) curve to dose-response data to extract EC50, Hill slope, and asymmetry, with AIC comparison against a standard 4PL fit and an interactive plot with residuals subplot.
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- **[Input File](./snippets/5-parameter-logistic/docs/input/dose_response_5pl_data.xlsx)**
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- **[Custom Code Block](./snippets/5-parameter-logistic/dose_response_5pl.py)**
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- **[Output Files](./snippets/5-parameter-logistic/docs/output)**
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## kinetic-timecourse
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Example snippet fits one-phase association kinetic curves per compound to extract parameters (Emax, kobs, t½, AUC, R²), with t-test comparison at the inflection point and a multi-compound time-course plot with 95% CI ribbons.
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- **[Input File](./snippets/kinetic-timecourse/docs/input/timecourse_data.xlsx)**
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- **[Custom Code Block](./snippets/kinetic-timecourse/kinetics_timecourse.py)**
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- **[Output Files](./snippets/kinetic-timecourse/docs/output)**
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## multi-group-anova
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Example snippet performs one-way ANOVA to test for significant differences across treatment groups, followed by Tukey HSD post-hoc analysis for pairwise comparisons, with descriptive statistics and an annotated bar chart output.
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- **[Input File](./snippets/multi-group-anova/docs/input/multigroup_efficacy_data.xlsx)**
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- **[Custom Code Block](./snippets/multi-group-anova/multigroup_anova.py)**
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- **[Output Files](./snippets/multi-group-anova/docs/output)**
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## plot-chromatogram
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Example snippet demonstrates plotting a multi-axis HPLC chromatogram (retention volume X Absorbance (mAU), Temperature (degC), pH) with custom code.
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allotropy==0.1.105
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biopython==1.86
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flowkit==0.1.0
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lmfit==1.3.4
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matplotlib==3.10.3
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numpy==2.2.4
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openpyxl==3.1.5
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pandas==2.2.3
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plotly==5.22.0
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pyarrow==19.0.1
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pydantic==2.12.5
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pydantic==1.10.21
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seaborn==0.13.2
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scikit-learn==1.6.1
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scipy==1.15.2
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statsmodels==0.14.4

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