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Clarify pipeline is COI-only, other barcodes are future adaptations
- Make it clear: currently ONLY supports COI for mosquitoes - Other barcodes (ITS, rbcL, 16S) are examples of POSSIBLE adaptations - Avoid confusion about current vs future capabilities
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README.md

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## Adaptable for Other Projects
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This pipeline is generic — use it for any Sanger sequencing project.
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**Current implementation:** COI barcoding for mosquito identification
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### Different Organisms
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**Potential adaptations:** This pipeline could be modified for other Sanger sequencing projects by replacing reference sequences and .ab1 files. The workflow (QC → Consensus → Alignment → Tree → BLAST) works for any organism with GenBank data.
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- Insects, plants, fungi, bacteria, fish, mammals
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- Any organism with reference sequences in GenBank
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### Possible Barcode Regions
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### Different Barcode Regions
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- **COI** (animals) — current default
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Examples of what this pipeline could be adapted for:
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- **ITS** (fungi)
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- **rbcL, matK** (plants)
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- **16S rRNA** (bacteria)
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- **COI** (other animals)
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### How to Customize
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### How to Adapt
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```bash
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# 1. Replace reference sequences
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./run-analysis.sh
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```
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BLAST automatically searches NCBI for any organism — species ID works for everything.
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BLAST automatically searches NCBI for any organism.
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