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config/_default/params.toml

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showDate = false
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showAuthor = false
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showTableOfContents = false
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content/about/_index.md

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## Impact
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Cytomining tools have been adopted across academia and industry for large-scale drug discovery and functional genomics.
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`pycytominer` underpins some of the largest publicly available image-based profiling datasets, including the [JUMP Cell Painting dataset](https://jump-cellpainting.broadinstitute.org/) (over 136,000 chemical and genetic perturbations profiled across 12 partner sites) the [LINCS Drug Repurposing](https://github.com/broadinstitute/lincs-cell-painting) Cell Painting dataset, and the [EU-OPENSCREEN](https://www.eu-openscreen.eu/) Bioactive Compound Set profiled across multiple imaging sites.
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`Pycytominer` underpins some of the largest publicly available image-based profiling datasets, including the [JUMP Cell Painting dataset](https://jump-cellpainting.broadinstitute.org/) (over 136,000 chemical and genetic perturbations profiled across 12 partner sites) the [LINCS Drug Repurposing](https://github.com/broadinstitute/lincs-cell-painting) Cell Painting dataset, and the [EU-OPENSCREEN](https://www.eu-openscreen.eu/) Bioactive Compound Set profiled across multiple imaging sites.
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It has also processed many of the 31+ datasets in the [Cell Painting Gallery](https://broadinstitute.github.io/cellpainting-gallery/).
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The foundational [Caicedo et al. 2017](https://doi.org/10.1038/nmeth.4397) review has accumulated over 670 citations, and individual tool papers have together been cited more than 150 times since 2024.
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In 2016, members of this community co-founded the [CytoData Society](https://www.cytodata.org/) to unite researchers across academia and industry around image-based profiling.
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The following year, the team contributed the landmark [Caicedo et al. 2017](https://doi.org/10.1038/nmeth.4397) review in _Nature Methods_ that established the field's foundational analysis standards.
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Since 2021, the [Way Lab](https://www.waysciencelab.com/) has driven a major expansion of the ecosystem — migrating from R to Python with `pycytominer` and building a modern infrastructure stack including `CytoTable` and `coSMicQC`.
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Since 2021, the [Way Lab](https://www.waysciencelab.com/) has driven a major expansion of the ecosystem — migrating from R to Python with `Pycytominer` and building a modern infrastructure stack including `CytoTable` and `coSMicQC`.
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Today, Cytomining tools are used by research groups worldwide for drug discovery, functional genomics, and cell biology.
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## Community

content/experimental/buscar.md

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<img class="logo-light" src="https://raw.githubusercontent.com/WayScience/buscar/main/logo/with-text-for-light-bg.png" alt="buscar logo" width="400">
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<img class="logo-dark" src="https://raw.githubusercontent.com/WayScience/buscar/main/logo/with-text-for-dark-bg.png" alt="buscar logo" width="400">
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**Problem:** Population-level hit calling averages away biologically meaningful cell-to-cell variation, making heterogeneous responses and rare subpopulations invisible to standard metrics.
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`buscar` scores perturbations directly on single-cell distributions using Earth Mover's Distance, preserving heterogeneity throughout hit calling.
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**Problem:** Population-level hit calling averages away biologically meaningful cell-to-cell variation, making heterogeneous responses and rare subpopulations invisible to standard metrics.
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**Key capabilities:**
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- Define on-target and off-target morphology signatures from reference profiles
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- Score perturbation efficacy via Earth Mover's Distance
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- Assess specificity with off-target scoring to reduce false positives
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- Preserve single-cell heterogeneity throughout hit calling
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- Integrates directly with `pycytominer`, `coSMicQC`, and `CytoTable` workflows
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- Integrates directly with `Pycytominer`, `coSMicQC`, and `CytoTable` workflows
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**[View on GitHub →](https://github.com/WayScience/buscar)**

content/experimental/iceberg-bioimage.md

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<img src="https://raw.githubusercontent.com/WayScience/iceberg-bioimage/main/docs/src/_static/iceberg-bioimage-logo.png" alt="iceberg-bioimage logo" width="400">
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**Problem:** Raw bioimaging archives have no standard catalog — finding, versioning, and joining images to downstream data requires bespoke scripts per lab.
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`iceberg-bioimage` scans any image store into a versioned Apache Iceberg catalog that directly exports Cytomining-compatible Parquet warehouses.
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**Problem:** Raw bioimaging archives have no standard catalog — finding, versioning, and joining images to downstream data requires bespoke scripts per lab.
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**Key capabilities:**
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- Scan image stores into canonical `ScanResult` objects
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- Validate profile tables against microscopy join contracts
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- Supports Zarr, OME-TIFF, and Parquet source formats
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**[View documentation →](https://wayscience.github.io/iceberg-bioimage/)**
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**[View documentation →](https://wayscience.github.io/iceberg-bioimage/)** · **[View on GitHub →](https://github.com/WayScience/iceberg-bioimage)**

content/experimental/ome-arrow.md

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<img src="https://raw.githubusercontent.com/WayScience/OME-arrow/main/docs/src/_static/ome-arrow-logo.png" alt="OME-arrow logo" width="400">
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**Problem:** Images and feature tables live in separate systems — linking a numeric outlier back to its source cell requires error-prone manual joins across formats.
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`OME-arrow` embeds images as first-class columns in Apache Arrow tables, so features, metadata, and pixel data travel together and can be queried or exported as tensors.
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**Problem:** Images and feature tables live in separate systems — linking a numeric outlier back to its source cell requires error-prone manual joins across formats.
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**Key capabilities:**
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- Store images, metadata, and derived features together in a single table
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- Tensor-focused output compatible with PyTorch, JAX, and DLPack
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- Visualization integrations for matplotlib, PyVista, and Napari
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**[View documentation →](https://wayscience.github.io/ome-arrow/)**
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**[View documentation →](https://wayscience.github.io/ome-arrow/)** · **[View on GitHub →](https://github.com/WayScience/OME-arrow)**

content/experimental/zedprofiler.md

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**Problem:** Classical profiling tools extract only 2D features, leaving organoid, cleared-tissue, and z-stack experiments without a CPU-efficient extractor.
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`zedprofiler` extracts morphological features directly from 3D volumetric images, including anisotropic voxel spacing correction — no GPU required.
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**Problem:** Classical profiling tools extract only 2D features, leaving organoid, cleared-tissue, and z-stack experiments without a CPU-efficient extractor.
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**Key capabilities:**
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- Extract features from 3D volumetric (z-stack) single-cell images

content/media/_index.md

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### Reviews
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- Serrano E, Peters J, Way GP et al. (2026) — Progress and new challenges in image-based
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profiling — _Molecular Systems Biology_
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[10.1038/s44320-026-00197-7](https://doi.org/10.1038/s44320-026-00197-7)
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- Caicedo JC, Cooper S, Singh S, Carpenter AE et al. (2017) — Data-analysis strategies for
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image-based cell profiling — _Nature Methods_ 14(9):849–863
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[10.1038/nmeth.4397](https://doi.org/10.1038/nmeth.4397)
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- Serrano, E. et al. Progress and new challenges in image-based profiling.
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*Mol. Syst. Biol.* **22**, 624–658 (2026).
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[doi:10.1038/s44320-026-00197-7](https://doi.org/10.1038/s44320-026-00197-7)
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- Caicedo, J.C. et al. Data-analysis strategies for image-based cell profiling.
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*Nat. Methods* **14**, 849–863 (2017).
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[doi:10.1038/nmeth.4397](https://doi.org/10.1038/nmeth.4397)
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### Tools
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- Serrano E, Chandrasekaran SN, Bunten D, Way GP et al. (2025) — Reproducible image-based
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profiling with Pycytominer — _Nature Methods_ 22:677–680
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[10.1038/s41592-025-02611-8](https://doi.org/10.1038/s41592-025-02611-8)
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- Kalinin AA, Arevalo J, Serrano E, Way GP, Singh S et al. (2025) — A versatile information
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retrieval framework for evaluating profile strength and similarity — _Nature Communications_
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[10.1038/s41467-025-60306-2](https://doi.org/10.1038/s41467-025-60306-2)
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- Moshkov N, Bornholdt M, Caicedo JC et al. (2024) — Learning representations for image-based
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profiling of perturbations — _Nature Communications_
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[10.1038/s41467-024-45999-1](https://doi.org/10.1038/s41467-024-45999-1)
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- Bunten D, Tomkinson J, Serrano E, Way GP et al. (2026) — Scalable data harmonization for
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single-cell image-based profiling with CytoTable — _Patterns_ 7(5):101514 —
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[10.1016/j.patter.2026.101514](https://doi.org/10.1016/j.patter.2026.101514)
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- Serrano E, Li WS, Way GP (2026) — Single-cell hit calling in high-content imaging screens
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with Buscar — _bioRxiv_ preprint —
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[10.64898/2026.04.15.718737](https://doi.org/10.64898/2026.04.15.718737)
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- Serrano E, Tomkinson J, Bunten D, Way GP et al. (2025) — Stellar quality control for
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single-cell image-based profiling with coSMicQC — _bioRxiv_ preprint —
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[10.1101/2025.10.14.682427](https://doi.org/10.1101/2025.10.14.682427)
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- Serrano, E. et al. Reproducible image-based profiling with Pycytominer.
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*Nat. Methods* **22**, 677–680 (2025).
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[doi:10.1038/s41592-025-02611-8](https://doi.org/10.1038/s41592-025-02611-8)
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- Kalinin, A.A. et al. A versatile information retrieval framework for evaluating profile strength and similarity.
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*Nat. Commun.* **16**, 5181 (2025).
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[doi:10.1038/s41467-025-60306-2](https://doi.org/10.1038/s41467-025-60306-2)
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- Moshkov, N. et al. Learning representations for image-based profiling of perturbations.
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*Nat. Commun.* **15**, 1594 (2024).
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[doi:10.1038/s41467-024-45999-1](https://doi.org/10.1038/s41467-024-45999-1)
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- Bunten, D. et al. Scalable data harmonization for single-cell image-based profiling with CytoTable.
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*Patterns* **7**, 101514 (2026).
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[doi:10.1016/j.patter.2026.101514](https://doi.org/10.1016/j.patter.2026.101514)
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- Serrano, E., Li, W.S. & Way, G.P. Single-cell hit calling in high-content imaging screens with Buscar.
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*bioRxiv* (2026).
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[doi:10.64898/2026.04.15.718737](https://doi.org/10.64898/2026.04.15.718737)
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- Tomkinson, J. et al. Stellar quality control for single-cell image-based profiling with coSMicQC.
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*bioRxiv* (2025).
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[doi:10.1101/2025.10.14.682427](https://doi.org/10.1101/2025.10.14.682427)

content/tools/copairs.md

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<a href="https://doi.org/10.1038/s41467-025-60306-2">A versatile information retrieval framework for evaluating profile strength and similarity</a>
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</p>
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<p style="font-size: 0.875rem; margin: 0.25rem 0 0; color: #374151;">
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Kalinin AA, Arevalo J, Serrano E, Vulliard L, Tsang H, et al.
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Kalinin, A.A. et al. <em>Nat. Commun.</em> <strong>16</strong>, 5181 (2025).
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</p>
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<p style="font-size: 0.8rem; margin: 0.25rem 0 0; color: #6b7280;">
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doi: <a href="https://doi.org/10.1038/s41467-025-60306-2" style="color: #6b7280;">10.1038/s41467-025-60306-2</a>

content/tools/cosmicqc.md

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- Flag over-segmented, under-segmented, and poorly focused cells
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- Apply threshold-based or z-score-based QC criteria
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- Generate summary reports of QC outcomes
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- Integrate seamlessly with `CytoTable` and `pycytominer` workflows
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- Integrate seamlessly with `CytoTable` and `Pycytominer` workflows
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**[View documentation →](https://cytomining.github.io/coSMicQC/)**
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<a href="https://doi.org/10.1101/2025.10.14.682427">Stellar quality control for single-cell image-based profiling with coSMicQC</a>
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</p>
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<p style="font-size: 0.875rem; margin: 0.25rem 0 0; color: #374151;">
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Tomkinson J, Bunten D, Way GP
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Tomkinson, J. et al. <em>bioRxiv</em> (2025).
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</p>
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<p style="font-size: 0.8rem; margin: 0.25rem 0 0; color: #6b7280;">
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doi: <a href="https://doi.org/10.1101/2025.10.14.682427" style="color: #6b7280;">10.1101/2025.10.14.682427</a>

content/tools/cytotable.md

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- Convert CellProfiler SQLite, CSV, and other formats into Parquet
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- Produce outputs compatible with `pycytominer` and AnnData workflows
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- Produce outputs compatible with `Pycytominer` and AnnData workflows
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**[View documentation →](https://cytomining.github.io/CytoTable/)**
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<a href="https://doi.org/10.1016/j.patter.2026.101514">Scalable data harmonization for single-cell image-based profiling with CytoTable</a>
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</p>
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<p style="font-size: 0.875rem; margin: 0.25rem 0 0; color: #374151;">
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Bunten D, Tomkinson J, Serrano E, Lippincott MJ, Brewer KI, et al.
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Bunten, D. et al. <em>Patterns</em> <strong>7</strong>, 101514 (2026).
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</p>
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<p style="font-size: 0.8rem; margin: 0.25rem 0 0; color: #6b7280;">
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doi: <a href="https://doi.org/10.1016/j.patter.2026.101514" style="color: #6b7280;">10.1016/j.patter.2026.101514</a>

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