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# DataJoint Element - Miniscope Calcium Imaging
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DataJoint Element for functional calcium imaging data acquired with the UCLA Miniscope and `Miniscope DAQ V4`acquisition system.
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DataJoint Element for functional calcium imaging data acquired with the [UCLA Miniscope](https://github.com/Aharoni-Lab/Miniscope-v4) and [Miniscope DAQ](https://github.com/Aharoni-Lab/Miniscope-DAQ-QT-Software)acquisition system, and analyzed with `CaImAn`.
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DataJoint Elements collectively standardize and automate data collection and analysis for neuroscience experiments. Each Element is a modular pipeline for data storage and processing with corresponding database tables that can be combined with other Elements to assemble a fully functional pipeline.
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Installation and usage instructions can be found at the
Copy file name to clipboardExpand all lines: docs/src/concepts.md
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## Miniscopes in Neuroscience Research
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Miniature fluorescence microscopes (miniscopes) are a head-mounted calcium imaging full-frame video modality first introduced in 2005 by Mark Schnitzer's lab ([Flusberg et al., Optics Letters 2005](https://pubmed.ncbi.nlm.nih.gov/16190441/)). Due to their light weight, these miniscopes allow measuring the dynamic activity of populations of cortical neurons in freely behaving animals. In 2011, Inscopix Inc. was founded to support one-photon miniscopes as a commercial neuroscience research platform, providing proprietary hardware, acquisition software, and analysis software. Today, they estimate their active user base is 491 labs with a total of 1179 installs. An open-source alternative was launched by a UCLA team led by Daniel Aharoni and Peyman Golshani ([Cai et al., Nature 2016](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5063500/); [Aharoni and Hoogland, Frontiers in Cellular Neuroscience 2019](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6461004/)). In our conversation with Dr. Aharoni, he estimated about 700 labs currently using the UCLA system alone. The Inscopix user base is smaller but more established. Several two-photon miniscopes have been developed but lack widespread adoption likely due to the expensive hardware required for the two-photon excitation ([Helmchen et al., Neuron 2001](https://pubmed.ncbi.nlm.nih.gov/11580892/); [Zong et al., Nature Methods 2017](https://pubmed.ncbi.nlm.nih.gov/28553965/); [Aharoni and Hoogland, Frontiers in Cellular Neuroscience 2019](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6461004/)). Due to the low costs and ability to record during natural behaviors, one-photon miniscope imaging appears to be the fastest growing calcium imaging modality in the field today. In Year 1, we focused our efforts on supporting the UCLA platform due its fast growth and deficiency of standardization in acquisition and processing pipelines. In future phases, we will reach out to Inscopix to support their platform as well.
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Miniature fluorescence microscopes (miniscopes) are a head-mounted calcium imaging full-frame video modality first introduced in 2005 by Mark Schnitzer's lab ([Flusberg et al., Optics Letters 2005](https://pubmed.ncbi.nlm.nih.gov/16190441/)). Due to their light weight, these miniscopes allow measuring the dynamic activity of populations of cortical neurons in freely behaving animals. In 2011, Inscopix Inc. was founded to support one-photon miniscopes as a commercial neuroscience research platform, providing proprietary hardware, acquisition software, and analysis software. Today, they estimate their active user base is 491 labs with a total of 1179 installs.
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An open-source alternative was launched by a UCLA team led by Drs. Daniel Aharoni and Peyman Golshani ([Cai et al., Nature 2016](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5063500/); [Aharoni and Hoogland, Frontiers in Cellular Neuroscience 2019](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6461004/)). In our conversation with Dr. Aharoni, he estimated about 700 labs currently using the UCLA system alone. The Inscopix user base is smaller but more established. Several two-photon miniscopes have been developed but lack widespread adoption likely due to the expensive hardware required for the two-photon excitation ([Helmchen et al., Neuron 2001](https://pubmed.ncbi.nlm.nih.gov/11580892/); [Zong et al., Nature Methods 2017](https://pubmed.ncbi.nlm.nih.gov/28553965/); [Aharoni and Hoogland, Frontiers in Cellular Neuroscience 2019](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6461004/)). Due to the low costs and ability to record during natural behaviors, one-photon miniscope imaging appears to be the fastest growing calcium imaging modality in the field today.
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The DataJoint team focused efforts on supporting the UCLA platform due rapid growth and limited standardization in acquisition and processing pipelines. In the future, we will reach out to Inscopix to support their platform as well.
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### Acquisition Tools
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Daniel Aharoni's lab has developed iterations of the UCLA Miniscope platform. Based on interviews, we have found labs using the two most recent versions including [Miniscope DAQ V3](http://miniscope.org/index.php/Information_on_the_(previous_Version_3)_Miniscope_platform) and [Miniscope DAQ V4](https://github.com/Aharoni-Lab/Miniscope-v4/wiki). Labs also use the Bonsai OpenEphys tool for data acquisition with the UCLA miniscope. Inscopix provides the Inscopix Data Acquisition Software (IDAS) for the nVista and nVoke systems.
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Dr. Daniel Aharoni's lab has developed iterations of the UCLA Miniscope platform. Based on interviews, we have found labs using the two most recent versions including [Miniscope DAQ V3](http://miniscope.org/index.php/Information_on_the_(previous_Version_3)_Miniscope_platform) and [Miniscope DAQ V4](https://github.com/Aharoni-Lab/Miniscope-v4/wiki). Labs also use the Bonsai OpenEphys tool for data acquisition with the UCLA miniscope. Inscopix provides the Inscopix Data Acquisition Software (IDAS) for the nVista and nVoke systems.
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### Preprocessing Tools
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The preprocessing workflow for miniscope imaging includes denoising, motion correction, cell segmentation, and calcium event extraction (sometimes described as "deconvolution" or "spike inference"). For the UCLA Miniscopes, the following [analysis packages](https://github.com/Aharoni-Lab/Miniscope-v4/wiki/Analysis-Packages) are commonly used:
| Segmentation | This table specifies the segmentation step and its outputs, following the motion correction step. |
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| Segmentation.Mask | This table contains the image mask for the segmented region of interest. |
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| MaskClassification | This table contains informmation about the classification of `Segmentation.Mask` into a type (e.g. soma, axon, dendrite, artifact, etc.). |
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| Fluorescence |The output fluorescence traces extracted from each `Segmentation.Mask`. |
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| ActivityExtractionMethod | A record of the activity extraction method (e.g. deconvolution) applied on the fluorescence trace. |
This Element features a DataJoint schema for functional calcium imaging data acquired with the UCLA Miniscope and `Miniscope DAQ V4`acquisition system, and analyzed with `CaImAn`. Each Element is a modular pipeline for data storage and processing with corresponding database tables that can be combined with other Elements to assemble a fully functional pipeline.
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DataJoint Element for functional calcium imaging data acquired with the [UCLA Miniscope](https://github.com/Aharoni-Lab/Miniscope-v4) and [Miniscope DAQ](https://github.com/Aharoni-Lab/Miniscope-DAQ-QT-Software)acquisition system, and analyzed with `CaImAn`. DataJoint Elements collectively standardize and automate data collection and analysis for neuroscience experiments. Each Element is a modular pipeline for data storage and processing with corresponding database tables that can be combined with other Elements to assemble a fully functional pipeline.
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Visit the [Concepts page](./concepts.md) for more information about the tables in the `miniscope` schema. To get started with building your data pipeline visit the [Tutorials page](./tutorials.md).
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Visit the [Concepts page](./concepts.md) for more information about miniscope calcium imaging and Element Miniscope. To get started with building your data pipeline visit the [Tutorials page](./tutorials.md).
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