diff --git a/newsfragments/+3f45d2d2.bugfix.rst b/newsfragments/+3f45d2d2.bugfix.rst
new file mode 100644
index 00000000..9f6f74b2
--- /dev/null
+++ b/newsfragments/+3f45d2d2.bugfix.rst
@@ -0,0 +1,2 @@
+Fixed an issue, where defining load paths in ini/toml configuration would fail
+due to pytest's LocalPath not having an endswith method.
diff --git a/pytest_postgresql/config.py b/pytest_postgresql/config.py
index 01f3e267..a772136e 100644
--- a/pytest_postgresql/config.py
+++ b/pytest_postgresql/config.py
@@ -3,6 +3,7 @@
 from pathlib import Path
 from typing import Any, List, Optional, TypedDict, Union
 
+from _pytest._py.path import LocalPath
 from pytest import FixtureRequest
 
 
@@ -50,10 +51,12 @@ def get_postgresql_option(option: str) -> Any:
     )
 
 
-def detect_paths(load_paths: List[str]) -> List[Union[Path, str]]:
+def detect_paths(load_paths: List[Union[LocalPath, str]]) -> List[Union[Path, str]]:
     """Convert path to sql files to Path instances."""
     converted_load_paths: List[Union[Path, str]] = []
     for path in load_paths:
+        if isinstance(path, LocalPath):
+            path = str(path)
         if path.endswith(".sql"):
             converted_load_paths.append(Path(path))
         else:
diff --git a/tests/test_config.py b/tests/test_config.py
new file mode 100644
index 00000000..aa39cb24
--- /dev/null
+++ b/tests/test_config.py
@@ -0,0 +1,22 @@
+"""Config tests."""
+
+from pathlib import Path
+from typing import Union
+
+import pytest
+from _pytest._py.path import LocalPath
+
+from pytest_postgresql.config import detect_paths
+
+
+@pytest.mark.parametrize(
+    "path, want",
+    (
+        ("test.sql", Path("test.sql")),
+        ("load.function", "load.function"),
+        (LocalPath("test.sql"), Path("test.sql").absolute()),  # type: ignore[no-untyped-call]
+    ),
+)
+def test_detect_paths(path: Union[str, LocalPath], want: Union[Path, str]) -> None:
+    """Check the correctness of detect_paths function."""
+    assert detect_paths([path]) == [want]
diff --git a/tests/test_postgres_options_plugin.py b/tests/test_postgres_options_plugin.py
index 1ba4bc27..c87fc3ea 100644
--- a/tests/test_postgres_options_plugin.py
+++ b/tests/test_postgres_options_plugin.py
@@ -48,6 +48,15 @@ def test_postgres_loader_in_cli(pointed_pytester: Pytester) -> None:
     ret.assert_outcomes(passed=1)
 
 
+def test_postgres_loader_in_ini(pointed_pytester: Pytester) -> None:
+    """Check that pytest.ini arguments are honored for load."""
+    pointed_pytester.copy_example("test_load.py")
+    test_sql_path = pointed_pytester.copy_example("test.sql")
+    pointed_pytester.makefile(".ini", pytest=f"[pytest]\npostgresql_load = {test_sql_path}\n")
+    ret = pointed_pytester.runpytest("test_load.py")
+    ret.assert_outcomes(passed=1)
+
+
 postgresql_proc_to_override = postgresql_proc()