diff --git a/haystack/core/serialization.py b/haystack/core/serialization.py
index 6342befbf35..39e6d112728 100644
--- a/haystack/core/serialization.py
+++ b/haystack/core/serialization.py
@@ -290,7 +290,10 @@ def default_from_dict(cls: type[T], data: dict[str, Any]) -> T:
     :raises DeserializationError:
         If the `type` field in `data` is missing or it doesn't match the type of `cls`.
     """
-    init_params = data.get("init_parameters", {})
+    # Copy so that replacing serialized sub-objects (Secret/ComponentDevice/nested components) with their
+    # deserialized instances below does not mutate the caller's ``data`` dict in place. Without this, a second
+    # deserialization of the same dict would receive already-parsed objects instead of their serialized form.
+    init_params = dict(data.get("init_parameters", {}))
     if "type" not in data:
         raise DeserializationError("Missing 'type' in serialization data")
     if data["type"] != generate_qualified_class_name(cls):
diff --git a/releasenotes/notes/fix-default-from-dict-input-mutation-e26045ee8d31e24d.yaml b/releasenotes/notes/fix-default-from-dict-input-mutation-e26045ee8d31e24d.yaml
new file mode 100644
index 00000000000..5f14c949949
--- /dev/null
+++ b/releasenotes/notes/fix-default-from-dict-input-mutation-e26045ee8d31e24d.yaml
@@ -0,0 +1,7 @@
+---
+fixes:
+  - |
+    Fixed ``default_from_dict`` (and therefore ``component_from_dict``) mutating the caller's ``data``
+    dictionary in place when deserializing nested objects such as ``Secret`` or ``ComponentDevice``.
+    The serialized sub-dictionaries were being replaced by their deserialized instances inside the input
+    dict; the input is now left unchanged, so the same ``data`` can be safely deserialized more than once.
diff --git a/test/core/test_serialization.py b/test/core/test_serialization.py
index af96b3dd3c4..614fa83147a 100644
--- a/test/core/test_serialization.py
+++ b/test/core/test_serialization.py
@@ -3,6 +3,8 @@
 # SPDX-License-Identifier: Apache-2.0
 
 import sys
+from copy import deepcopy
+from typing import Any
 from unittest.mock import Mock
 
 import pytest
@@ -231,6 +233,24 @@ def test_component_from_dict_with_secret():
     assert comp.regular_param == "test"
 
 
+def test_component_from_dict_does_not_mutate_input():
+    """default_from_dict must not mutate the caller's data dict when deserializing nested objects."""
+    serialized_secret = Secret.from_env_var("TEST_API_KEY").to_dict()
+    data: dict[str, Any] = {
+        "type": generate_qualified_class_name(CustomComponentWithSecrets),
+        "init_parameters": {"api_key": serialized_secret, "regular_param": "test"},
+    }
+    expected = deepcopy(data)
+
+    comp = component_from_dict(CustomComponentWithSecrets, data, "test_component")
+
+    # the returned component is correctly deserialized ...
+    assert isinstance(comp.api_key, Secret)
+    # ... but the caller's dict is left untouched (the Secret sub-dict is not replaced in place)
+    assert data == expected
+    assert isinstance(data["init_parameters"]["api_key"], dict)
+
+
 def test_component_to_dict_and_from_dict_roundtrip_with_secret():
     """Test that serialization and deserialization work together for Secrets."""
     # Test roundtrip with EnvVarSecret