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fix conflicts
2 parents 7c3cc7b + 79760b8 commit 511ee77

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.gitignore

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*.egg
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*.egg-info
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dist
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build
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eggs
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parts
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bin

conf.py

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# List of patterns, relative to source directory, that match files and
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# directories to ignore when looking for source files.
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exclude_patterns = ['_build']
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exclude_patterns = ['_build','env']
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# The reST default role (used for this markup: `text`) to use for all documents.
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#default_role = None

metagenomics/2-diginorm.txt

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.pe.qc.fq.gz.keep.abundfilt.se). (Yes, the naming scheme does make
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sense. Trust me.)
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You can now remove the ``normC20k20.kh`` file, too
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::
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rm normC20k20.kh
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Normalize Down to C=5
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---------------------

metagenomics/4-assemble.txt

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At last! All that filtering and diginorming is done, and we can get
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down to the serious business of assembling. Huzzah!
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Let's start with some collection of group files in ``/mnt/assembly``.
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.. shell start
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do
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name=$(basename $i .fq).fa
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python /usr/local/share/khmer/scripts/fastq-to-fasta.py $i > $name
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name=$(basename $i .fq.gz).fa
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python /usr/local/share/khmer/sandbox/fastq-to-fasta.py $i > $name
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done
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Setting Up the Assemblies

misc/variant.txt

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Variant calling
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###############
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================
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We are using Podar dataset from ...
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cp bwa /usr/local/bin
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.. We also need a new version of `samtools <http://samtools.sourceforge.net/>`__::
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We also need a new version of `samtools <http://samtools.sourceforge.net/>`__::
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cd /root
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curl -O -L http://sourceforge.net/projects/samtools/files/samtools/0.1.19/samtools-0.1.19.tar.bz2
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cp *.pl maq2sam-long maq2sam-short md5fa md5sum-lite wgsim /usr/local/bin/
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Download data
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~~~~~~~~~~~~~
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=============
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Download the reference genome and the resequencing reads::
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mrnaseq/0-download-and-save.txt

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--------------------------------------------
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There are *many* different ways of getting big sequence files to and
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from Amazon. The two that I mostly use are 'curl', which downloads
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files from a Web site URL; and 'ncftp', which is a robust FTP client
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from Amazon. The two that I mostly use are ``curl``, which downloads
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files from a Web site URL; and ``ncftp``, which is a robust FTP client
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that let's you get files from an FTP site. Sequencing centers almost
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always make their data available in one of these two ways.
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cd /mnt
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ncftp -u <username> ftp://path/to/FTP/site
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use 'cd' to find the right directory, and then::
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use ``cd`` to find the right directory, and then::
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>> mget *
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to download the files. Then type 'quit'.
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You can also use 'curl' to download files one at a time from Web or FTP sites.
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You can also use ``curl`` to download files one at a time from Web or FTP sites.
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For example, to save a file from a website, you could use::
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cd /mnt
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curl -O http://path/to/file/on/website
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Once you have the files, figure out their size using 'du -sk' (e.g. after the
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above, 'du -sk /mnt' will tell you how much data you have saved under /mnt),
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Once you have the files, figure out their size using ``du -sh`` (e.g. after the
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above, ``du -sh /mnt`` will tell you how much data you have saved under /mnt),
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and go create and attach a volume (see :doc:`../amazon/index`).
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Any files in the '/mnt' directory will be lost when the instance is stopped or

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