diff --git a/README.md b/README.md
index ae77df4651..5a6e923a03 100644
--- a/README.md
+++ b/README.md
@@ -249,7 +249,6 @@ rules in templates can be disabled with eslint directives with mustache or html
 | [template-no-model-argument-in-route-templates](docs/rules/template-no-model-argument-in-route-templates.md)       | disallow @model argument in route templates                                  |    | 🔧 |    |
 | [template-no-multiple-empty-lines](docs/rules/template-no-multiple-empty-lines.md)                                 | disallow multiple consecutive empty lines in templates                       |    | 🔧 |    |
 | [template-no-mut-helper](docs/rules/template-no-mut-helper.md)                                                     | disallow usage of (mut) helper                                               |    |    |    |
-| [template-no-negated-comparison](docs/rules/template-no-negated-comparison.md)                                     | disallow negated comparisons in templates                                    |    |    |    |
 | [template-no-negated-condition](docs/rules/template-no-negated-condition.md)                                       | disallow negated conditions in if/unless                                     |    | 🔧 |    |
 | [template-no-nested-splattributes](docs/rules/template-no-nested-splattributes.md)                                 | disallow nested ...attributes usage                                          |    |    |    |
 | [template-no-obscure-array-access](docs/rules/template-no-obscure-array-access.md)                                 | disallow obscure array access patterns like `objectPath.@each.property`      |    | 🔧 |    |
diff --git a/docs/rules/template-no-negated-comparison.md b/docs/rules/template-no-negated-comparison.md
deleted file mode 100644
index 2b46e145b5..0000000000
--- a/docs/rules/template-no-negated-comparison.md
+++ /dev/null
@@ -1,39 +0,0 @@
-# ember/template-no-negated-comparison
-
-<!-- end auto-generated rule header -->
-
-Disallows negated comparison operators in templates.
-
-## Rule Details
-
-Use positive comparison operators with `{{unless}}` instead of negated comparison operators like `not-eq`.
-
-## Examples
-
-Examples of **incorrect** code for this rule:
-
-```gjs
-<template>
-  {{#if (not-eq this.value 5)}}
-    Not equal
-  {{/if}}
-</template>
-```
-
-Examples of **correct** code for this rule:
-
-```gjs
-<template>
-  {{#unless (eq this.value 5)}}
-    Not equal
-  {{/unless}}
-</template>
-```
-
-```gjs
-<template>
-  {{#if (eq this.value 5)}}
-    Equal
-  {{/if}}
-</template>
-```
diff --git a/lib/rules/template-no-negated-comparison.js b/lib/rules/template-no-negated-comparison.js
deleted file mode 100644
index 6ae92584e8..0000000000
--- a/lib/rules/template-no-negated-comparison.js
+++ /dev/null
@@ -1,34 +0,0 @@
-/** @type {import('eslint').Rule.RuleModule} */
-module.exports = {
-  meta: {
-    type: 'suggestion',
-    docs: {
-      description: 'disallow negated comparisons in templates',
-      category: 'Best Practices',
-      url: 'https://github.com/ember-cli/eslint-plugin-ember/tree/master/docs/rules/template-no-negated-comparison.md',
-      templateMode: 'both',
-    },
-    fixable: null,
-    schema: [],
-    messages: {
-      noNegatedComparison: 'Use positive comparison operators instead of negated ones.',
-    },
-  },
-
-  create(context) {
-    return {
-      GlimmerSubExpression(node) {
-        if (
-          node.path &&
-          node.path.type === 'GlimmerPathExpression' &&
-          node.path.original === 'not-eq'
-        ) {
-          context.report({
-            node,
-            messageId: 'noNegatedComparison',
-          });
-        }
-      },
-    };
-  },
-};
diff --git a/tests/lib/rules/template-no-negated-comparison.js b/tests/lib/rules/template-no-negated-comparison.js
deleted file mode 100644
index c6a99f8045..0000000000
--- a/tests/lib/rules/template-no-negated-comparison.js
+++ /dev/null
@@ -1,68 +0,0 @@
-//------------------------------------------------------------------------------
-// Requirements
-//------------------------------------------------------------------------------
-
-const rule = require('../../../lib/rules/template-no-negated-comparison');
-const RuleTester = require('eslint').RuleTester;
-
-//------------------------------------------------------------------------------
-// Tests
-//------------------------------------------------------------------------------
-
-const ruleTester = new RuleTester({
-  parser: require.resolve('ember-eslint-parser'),
-  parserOptions: { ecmaVersion: 2022, sourceType: 'module' },
-});
-
-ruleTester.run('template-no-negated-comparison', rule, {
-  valid: [
-    `<template>
-      {{#if (eq this.value 5)}}
-        Equal
-      {{/if}}
-    </template>`,
-    `<template>
-      {{#unless (eq this.value 5)}}
-        Not equal
-      {{/unless}}
-    </template>`,
-    `<template>
-      <div></div>
-    </template>`,
-    // `ne` is not a standard Ember/ember-truth-helpers helper; the rule must not flag it.
-    `<template>
-      {{#if (ne this.a this.b)}}
-        Not equal
-      {{/if}}
-    </template>`,
-  ],
-
-  invalid: [
-    {
-      code: `<template>
-        {{#if (not-eq this.value 5)}}
-          Not equal
-        {{/if}}
-      </template>`,
-      output: null,
-      errors: [
-        {
-          message: 'Use positive comparison operators instead of negated ones.',
-          type: 'GlimmerSubExpression',
-        },
-      ],
-    },
-    {
-      code: `<template>
-        <div class={{if (not-eq this.state "active") "inactive"}}></div>
-      </template>`,
-      output: null,
-      errors: [
-        {
-          message: 'Use positive comparison operators instead of negated ones.',
-          type: 'GlimmerSubExpression',
-        },
-      ],
-    },
-  ],
-});