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foci-analyzer: add missing front matter
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# Foci-analyzer
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title: Foci Analyzer
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categories: [Analysis]
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ImageJ macro for the analysis of foci (e.g. DNA damage) in nuclei (or cells). Works on 2D/3D fluorescence images, including multiseries files, as long as all series have the same dimensions.
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Timelapse images are split into separate timepoints and processed individually.
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![image](https://user-images.githubusercontent.com/68109112/180581530-dd326026-cc74-4ce1-8d97-14518bfd4d73.png)
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## Workflow summary
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1. Nuclei are segmented using the pre-trained deep learning network [StarDist](https://imagej.net/plugins/stardist) (2D / 2D projection). Alternatively, the deep learning network [Cellpose](https://github.com/MouseLand/cellpose) can be used to segment nuclei or whole cells (2D, 2.5D or 3D), thanks to the [Cellpose wrapper for Fiji](https://github.com/BIOP/ijl-utilities-wrappers) by BIOP. Alternatively, classic thresholding + watershedding can be used (though no parameters can be changed).
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2. Foci are detected in each nucleus in 2D or 3D. After Difference of Gaussians background subtraction, local maxima are detected and used as seeds for [MorpholibJ](https://imagej.net/plugins/morpholibj)'s [marker-controlled watershed](https://imagej.net/plugins/marker-controlled-watershed) (executed on the GPU using [CLIJ2/CLIJx](https://clij.github.io/)). Additionally, AreaMaxima local maxima detection can be used as detection method.

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