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# Overview
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The phylogenetic tree was generated from all complete *Lyssavirus rabies* genomes obtained from [NCBI Nucleotide](https://ftp.ncbi.nlm.nih.gov/genomes/Viruses/AllNuclMetadata/) (accessed 2025/05/01) with > 95% unambiguous nucleotides and < 15,000 bp. Clade, subclade, and other associated metadata were acquired referencing the original rabies Nextstrain build and [RABV-GLUE's metadata](https://github.com/giffordlabcvr/RABV-GLUE/blob/master/tabular/reference-set-data.tsv). Clade- and subclade-specific mutations [were extracted for all monophyletic major and minor lineages](https://github.com/theiagen/utilities/pull/21), and disregarded for previously assigned clades that were not monophyletic in the phylogeny. Complete genome assemblies were aligned with `mafft v7.525` using default parameters and the phylogeny was reconstructed using `iqtree v2.4.0` with 1000 ultrafast bootstrap iterations and GTR+F+I+R7 selected as the evolutionary model by the *ModelFinder* module. A root node internal to the *L. rabies* phylogeny was selected by reconstructing an additional phylogeny using the same methodology with *L. australis* (NC_003243.1) and *L. gannoruwa* (KU244266.2) included as known outgroups ([Baynard & Fooks, 2021](doi.org/10.1016/B978-0-12-809633-8.20936-9)).
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The phylogenetic tree was generated from all complete *Lyssavirus rabies* genomes obtained from [NCBI Nucleotide](https://ftp.ncbi.nlm.nih.gov/genomes/Viruses/AllNuclMetadata/) (accessed 2025/05/01) with > 95% unambiguous nucleotides and < 15,000 bp. Clade, subclade, and other associated metadata were acquired referencing the original rabies Nextstrain build and [RABV-GLUE's metadata](https://github.com/giffordlabcvr/RABV-GLUE/blob/master/tabular/reference-set-data.tsv). Clade- and subclade-specific mutations [were extracted for all monophyletic major and minor lineages](https://github.com/theiagen/utilities/pull/21), and disregarded for previously assigned clades that were not monophyletic in the phylogeny. Complete genome assemblies were aligned with `mafft v7.525` using default parameters and the phylogeny was reconstructed using `iqtree v2.4.0` with 1000 ultrafast bootstrap iterations and GTR+F+I+R7 selected as the evolutionary model by the *ModelFinder* module. A root node internal to the *L. rabies* phylogeny was selected by reconstructing an additional phylogeny using the same methodology with *L. australis* (NC_003243.1) and *L. gannoruwa* (KU244266.2) included as known outgroups ([Baynard & Fooks, 2021](https://doi.org/10.1016/B978-0-12-809633-8.20936-9)).
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# Metadata acquisition
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