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19 changes: 19 additions & 0 deletions README.md
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Expand Up @@ -16,6 +16,7 @@ The test data includes the following:
- Samplesheets (both with and without Space Ranger pre-processing)
- Processed Space Ranger data for downstream testing
- Compressed archives for downloading during CI testing
- Full-size samplesheets for the full-size test profiles

## Origins

Expand Down Expand Up @@ -65,6 +66,24 @@ cp CytAssist_11mm_FFPE_Human_Glioblastoma_image.tif sub-sampled/
for f in CytAssist_11mm_FFPE_Human_Glioblastoma_fastqs/*S1*L00{1,2}*R*; do; gzip -cdf $f | head -n 40000 | gzip -c > sub-sampled/$(basename $f); done
```

## Full-size test data

In addition to the sub-sampled CI test data above, each test-data directory
provides a `samplesheet_full.csv` that drives the pipeline's full-size test
profiles (`test_full`, `test_full_v1` and `test_full_hd`). Unlike the
sub-sampled data, these samplesheets reference the **original, full-size**
inputs (FASTQ `.tar` archives, tissue images and slide `.gpr` files) and stream
them directly from 10x Genomics. Only the small samplesheets are hosted in this
branch — no heavy files are stored here.

The full-size samplesheets cover the following datasets:

| Samplesheet | Dataset | Chemistry |
| ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | ------------------------------------------------------ | --------- |
| [`human-ovarian-cancer-1-standard_v1_ffpe/samplesheet_full.csv`](testdata/human-ovarian-cancer-1-standard_v1_ffpe/samplesheet_full.csv) | Human ovarian cancer 1, FFPE direct placement | Visium v1 |
| [`human-brain-cancer-11-mm-capture-area-ffpe-2-standard_v2_ffpe_cytassist/samplesheet_full.csv`](testdata/human-brain-cancer-11-mm-capture-area-ffpe-2-standard_v2_ffpe_cytassist/samplesheet_full.csv) | Human glioblastoma, 11 mm capture area, FFPE CytAssist | Visium v2 |
| [`human-lung-cancer-post-xenium_hd_ffpe/samplesheet_full.csv`](testdata/human-lung-cancer-post-xenium_hd_ffpe/samplesheet_full.csv) | Human lung cancer (post-Xenium), FFPE | Visium HD |

## Support

For further information or help, don't hesitate to get in touch on our
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sample,fastq_dir,cytaimage,slide,area,manual_alignment,slidefile
CytAssist_11mm_FFPE_Human_Glioblastoma_2,https://s3-us-west-2.amazonaws.com/10x.files/samples/spatial-exp/2.0.1/CytAssist_11mm_FFPE_Human_Glioblastoma/CytAssist_11mm_FFPE_Human_Glioblastoma_fastqs.tar,https://cf.10xgenomics.com/samples/spatial-exp/2.0.1/CytAssist_11mm_FFPE_Human_Glioblastoma/CytAssist_11mm_FFPE_Human_Glioblastoma_image.tif,V52Y10-317,B1,,https://s3.us-west-2.amazonaws.com/10x.spatial-slides/gpr/V52Y10/V52Y10-317.gpr
Comment thread
mashehu marked this conversation as resolved.
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sample,fastq_dir,image,cytaimage,slide,area,manual_alignment,slidefile
Visium_HD_Human_Lung_Cancer_HD_Only_Experiment2,https://s3-us-west-2.amazonaws.com/10x.files/samples/spatial-exp/3.0.0/Visium_HD_Human_Lung_Cancer_HD_Only_Experiment2/Visium_HD_Human_Lung_Cancer_HD_Only_Experiment2_fastqs.tar,https://cf.10xgenomics.com/samples/spatial-exp/3.0.0/Visium_HD_Human_Lung_Cancer_HD_Only_Experiment2/Visium_HD_Human_Lung_Cancer_HD_Only_Experiment2_tissue_image.btf,https://cf.10xgenomics.com/samples/spatial-exp/3.0.0/Visium_HD_Human_Lung_Cancer_HD_Only_Experiment2/Visium_HD_Human_Lung_Cancer_HD_Only_Experiment2_image.tif,H1-84QJZFR,D1,,
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sample,fastq_dir,image,slide,area,manual_alignment,slidefile
Visium_FFPE_Human_Ovarian_Cancer,https://cf.10xgenomics.com/samples/spatial-exp/1.3.0/Visium_FFPE_Human_Ovarian_Cancer/Visium_FFPE_Human_Ovarian_Cancer_fastqs.tar,https://cf.10xgenomics.com/samples/spatial-exp/1.3.0/Visium_FFPE_Human_Ovarian_Cancer/Visium_FFPE_Human_Ovarian_Cancer_image.jpg,V10L13-020,D1,,https://s3.us-west-2.amazonaws.com/10x.spatial-slides/gpr/V10L13/V10L13-020.gpr