diff --git a/instrumentation-genai/opentelemetry-instrumentation-openai-v2/src/opentelemetry/instrumentation/openai_v2/utils.py b/instrumentation-genai/opentelemetry-instrumentation-openai-v2/src/opentelemetry/instrumentation/openai_v2/utils.py
index 6afca130b6..8ed425f816 100644
--- a/instrumentation-genai/opentelemetry-instrumentation-openai-v2/src/opentelemetry/instrumentation/openai_v2/utils.py
+++ b/instrumentation-genai/opentelemetry-instrumentation-openai-v2/src/opentelemetry/instrumentation/openai_v2/utils.py
@@ -37,6 +37,7 @@
 )
 from opentelemetry.trace.status import Status, StatusCode
 from opentelemetry.util.genai.types import (
+    ContentCapturingMode,
     InputMessage,
     LLMInvocation,
     OutputMessage,
@@ -55,7 +56,16 @@ def is_content_enabled() -> bool:
         OTEL_INSTRUMENTATION_GENAI_CAPTURE_MESSAGE_CONTENT, "false"
     )
 
-    return capture_content.lower() == "true"
+    lower = capture_content.lower()
+    if lower == "true":
+        return True
+
+    # Support newer ContentCapturingMode enum values (e.g. span_and_event)
+    try:
+        mode = ContentCapturingMode[lower.upper()]
+        return mode != ContentCapturingMode.NO_CONTENT
+    except KeyError:
+        return False
 
 
 def extract_tool_calls(item, capture_content):
diff --git a/instrumentation-genai/opentelemetry-instrumentation-openai-v2/tests/test_is_content_enabled.py b/instrumentation-genai/opentelemetry-instrumentation-openai-v2/tests/test_is_content_enabled.py
new file mode 100644
index 0000000000..f44b9e5e0b
--- /dev/null
+++ b/instrumentation-genai/opentelemetry-instrumentation-openai-v2/tests/test_is_content_enabled.py
@@ -0,0 +1,70 @@
+# Copyright The OpenTelemetry Authors
+#
+# Licensed under the Apache License, Version 2.0 (the "License");
+# you may not use this file except in compliance with the License.
+# You may obtain a copy of the License at
+#
+#     http://www.apache.org/licenses/LICENSE-2.0
+#
+# Unless required by applicable law or agreed to in writing, software
+# distributed under the License is distributed on an "AS IS" BASIS,
+# WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
+# See the License for the specific language governing permissions and
+# limitations under the License.
+
+"""Unit tests for is_content_enabled() covering all ContentCapturingMode values."""
+
+import pytest
+
+from opentelemetry.instrumentation.openai_v2.utils import (
+    OTEL_INSTRUMENTATION_GENAI_CAPTURE_MESSAGE_CONTENT,
+    is_content_enabled,
+)
+
+
+@pytest.mark.parametrize(
+    "env_value",
+    ["true", "True", "TRUE"],
+)
+def test_is_content_enabled_true(monkeypatch, env_value):
+    monkeypatch.setenv(OTEL_INSTRUMENTATION_GENAI_CAPTURE_MESSAGE_CONTENT, env_value)
+    assert is_content_enabled() is True
+
+
+def test_is_content_enabled_span_and_event(monkeypatch):
+    monkeypatch.setenv(OTEL_INSTRUMENTATION_GENAI_CAPTURE_MESSAGE_CONTENT, "span_and_event")
+    assert is_content_enabled() is True
+
+
+def test_is_content_enabled_span_only(monkeypatch):
+    monkeypatch.setenv(OTEL_INSTRUMENTATION_GENAI_CAPTURE_MESSAGE_CONTENT, "span_only")
+    assert is_content_enabled() is True
+
+
+def test_is_content_enabled_event_only(monkeypatch):
+    monkeypatch.setenv(OTEL_INSTRUMENTATION_GENAI_CAPTURE_MESSAGE_CONTENT, "event_only")
+    assert is_content_enabled() is True
+
+
+@pytest.mark.parametrize(
+    "env_value",
+    ["false", "False", "FALSE"],
+)
+def test_is_content_enabled_false(monkeypatch, env_value):
+    monkeypatch.setenv(OTEL_INSTRUMENTATION_GENAI_CAPTURE_MESSAGE_CONTENT, env_value)
+    assert is_content_enabled() is False
+
+
+def test_is_content_enabled_empty(monkeypatch):
+    monkeypatch.setenv(OTEL_INSTRUMENTATION_GENAI_CAPTURE_MESSAGE_CONTENT, "")
+    assert is_content_enabled() is False
+
+
+def test_is_content_enabled_random_string(monkeypatch):
+    monkeypatch.setenv(OTEL_INSTRUMENTATION_GENAI_CAPTURE_MESSAGE_CONTENT, "random")
+    assert is_content_enabled() is False
+
+
+def test_is_content_enabled_unset(monkeypatch):
+    monkeypatch.delenv(OTEL_INSTRUMENTATION_GENAI_CAPTURE_MESSAGE_CONTENT, raising=False)
+    assert is_content_enabled() is False