Skip to content

Commit 76dc9de

Browse files
authored
Update Optical_Alignment_Procedures.md
1 parent 5b9ffd7 commit 76dc9de

1 file changed

Lines changed: 15 additions & 15 deletions

File tree

docs/03_Investigator/02_FRAME/config_examples/Optical_Alignment_Procedures.md

Lines changed: 15 additions & 15 deletions
Original file line numberDiff line numberDiff line change
@@ -2,31 +2,31 @@
22

33
## Overview
44

5-
The FRAME system requires precise optical alignment to ensure optimal performance. This document provides detailed procedures for aligning the internal optics cube and calibrating the optical path.
5+
The FRAME system requires precise optical alignment to ensure optimal performance. This document provides detailed procedures for aligning the internal optics module and calibrating the optical path.
66

77
## Internal Optics Cube Preparation
88

99
### Initial Setup Outside the FRAME
1010

11-
Before installing the internal optics cube in the FRAME, it must be properly aligned using a separate alignment setup.
11+
Before installing the internal optics module in the FRAME, it must be properly aligned using a separate alignment setup.
1212

1313
#### Components to Align
1414

15-
The internal optics cube contains three main optical elements that must be aligned:
15+
The internal optics module contains three main optical elements that must be aligned:
1616
1. **Transmitted light module** with camera and folding mirror
1717
2. **Fluorescence excitation module**
1818
3. **Objective mounting system**
1919

2020
#### Alignment Setup
2121

2222
1. **Create External Test Setup**:
23-
- Remove the internal optics cube from the FRAME
24-
- Build a test setup on top of the cube that mimics the FRAME's optical distances
23+
- Remove the internal optics module from the FRAME
24+
- Build a test setup on top of the module that mimics the FRAME's optical distances
2525
- Mount an objective in the test setup to maintain proper optical spacing
2626

2727
2. **Software Preparation**:
2828
- Start ImSwitch software to enable camera viewing
29-
- Ensure proper communication with the cube's electronics
29+
- Ensure proper communication with the module's electronics
3030

3131
### Fluorescence Module Alignment
3232

@@ -48,12 +48,12 @@ The internal optics cube contains three main optical elements that must be align
4848
#### Mechanical Alignment
4949

5050
1. **Access Adjustment Screws**:
51-
- Use a 2.5mm hex key to access the three adjustment screws on the back of the cube
51+
- Use a 2.5mm hex key to access the three adjustment screws on the back of the module
5252
- These screws control the position and angle of the optical elements
5353

5454
2. **Laser Centering**:
5555
- Ensure the laser focus is exactly centered where the objective will be positioned
56-
- The light beam must exit the internal cube parallel to the optical axis
56+
- The light beam must exit the internal module parallel to the optical axis
5757
- This ensures proper Köhler illumination
5858

5959
3. **Objective Installation**:
@@ -70,13 +70,13 @@ The internal optics cube contains three main optical elements that must be align
7070

7171
### Objective-to-FRAME Alignment
7272

73-
After internal cube alignment, align the optical axis of the FRAME with the internal cube.
73+
After internal module alignment, align the optical axis of the FRAME with the internal module.
7474

7575
#### Available Adjustments
7676

7777
Two adjustment mechanisms are available:
7878
1. **X-axis**: Objective can be moved along the X-axis using the slide mechanism
79-
2. **Y-axis**: Internal cube can be moved relative to the FRAME using adjustment screws
79+
2. **Y-axis**: Internal module can be moved relative to the FRAME using adjustment screws
8080

8181
#### LED Alignment Tool Setup
8282

@@ -97,7 +97,7 @@ Two adjustment mechanisms are available:
9797
- Observe the point light source projected to the back focal plane
9898

9999
2. **Y-axis Alignment**:
100-
- Adjust the internal cube position along Y-axis using the adjustment screw
100+
- Adjust the internal module position along Y-axis using the adjustment screw
101101
- Move the light point to the center of the back focal plane
102102
- Use a piece of paper with crosshairs to mark the position
103103

@@ -133,9 +133,9 @@ Two adjustment mechanisms are available:
133133

134134
Once all optical elements are properly aligned:
135135

136-
1. **Secure Internal Cube**:
137-
- Tighten the locking knobs to permanently secure the internal cube in the rail
138-
- Ensure the cube cannot move during operation
136+
1. **Secure Internal Module**:
137+
- Tighten the locking knobs to permanently secure the internal module in the rail
138+
- Ensure the module cannot move during operation
139139

140140
2. **System Testing**:
141141
- Perform comprehensive testing of all optical functions
@@ -171,4 +171,4 @@ Once all optical elements are properly aligned:
171171
- Regular cleaning of optical surfaces
172172
- Periodic verification of alignment
173173
- Check mechanical adjustments for wear
174-
- Update position calibrations as needed
174+
- Update position calibrations as needed

0 commit comments

Comments
 (0)