adding fluorescence microscope and goniometer

Author: beniroquai

docs/usage/apps/README.md

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+# openUC2 Goniometer 
+
+
+
+![alt text](./IMAGES/IMG_20260430_103804.jpg) 
+
+![](./IMAGES/IMG_20260430_104047.jpg)
+
+![](./IMAGES/contact_angle_result_1777538382521.jpg)
+
+![](./IMAGES/droplet.png)

docs/usage/apps/goniometer/IMAGES/IMG_20260430_103804.jpg

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+# Smartphone Fluorescence Microscope
+
+This tutorial gives an idea how easy it can be to build a fluorescence microscope. It uses a high power LED with the appropriate filter set to for example image GFP-labelled samples. Here, we give you a quick intro how to setup the device. **Disclaimer:** This Tutorial uses the white light source from the coreBOX, but you will get better contrast with the fluorescence high power LED. 
+
+
+## Tutorial
+
+The is written by Lara Pötsch from the Friedrich Schiller University! Thanks! :) 
+
+
+### Necessary Materials:
+
+a)  three 45°-mirrors
+b)  dichroic mirror
+c)  infinity-corrected objective
+d)  eyepiece
+e)  manual z-stage
+f)  sample holder
+g)  three empty cubes
+h)  torch
+i)  torch holder (optional)
+j)  phone holder (optional)
+k)  eleven base plates
+l)  light diffuser (optional)
+m)  fluorescent sample
+n)  smartphone/camera
+
+![](./IMAGES/smartphonemicroscope/image1.jpeg)
+
+## Diagram:
+
+<iframe
+  src="https://youseetoo.github.io/configurator/viewer/?json=https://github.com/beniroquai/openUC2-OptiKit-Store/raw/7f2a476366afaeb70b4769c8aa5aa9388228cfe6/setups/setup-1758601890024.json"
+  width="100%"
+  height="500"
+  frameborder="0"
+  style="border-radius: 8px; border: 1px solid #ddd;"
+></iframe>
+
+
+## Building the microscope bottom to top:
+
+1.  **Building the base plate:** Arrange 5 base plates like the image
+    below. There the positions are labelled 1 through 4 for reference.
+    This will create the ground structure of the microscope and connect
+    the cubes for the mirrors, including the dichroic mirror, as well as
+    the stage and detection unit.
+
+![](./IMAGES/smartphonemicroscope/image2.jpeg)
+
+1.  **Finding the right orientation for the dichroic mirror:** The
+    dichroic mirror has 3 circular cut-outs. One contains a
+    yellow-looking and another one a blue-looking filter -- the
+    respective colours are reflected and not transmitted. Since we are
+    working with fluorescence, the excitation filter should pass lower
+    wavelengths than the emission filter. Therefore, the yellow-looking
+    but blue-transmitting filter should point towards the light source.
+    The excitation light is then reflected towards the circular opening
+    without a filter. In this direction the stage unit should be placed.
+    The detection unit is built in the direction of the blue-looking but
+    green/yellow-transmitting filter.
+
+![](./IMAGES/smartphonemicroscope/image3.jpeg)
+
+![](./IMAGES/smartphonemicroscope/image4.jpeg)
+
+![](./IMAGES/smartphonemicroscope/image5.jpeg)
+
+
+1.  **Placing the lowest row of components:** Place a 45°-mirror on the
+    1^st^ and 3^rd^ base plate so that the mirrors point to each other
+    like in the images below. Add a third 45°-mirror on position 2.2 in
+    direction of base plate 2.1. Stick the dichroic mirror in between
+    the 45°-mirrors so that the circular opening without a filter points
+    to base plate 1 and the yellow-looking filter points to base plate
+    2.2. Next, click an empty cube on base plate 4.
+
+![](./IMAGES/smartphonemicroscope/image6.jpeg)
+
+![](./IMAGES/smartphonemicroscope/image7.jpeg)
+
+![](./IMAGES/smartphonemicroscope/image8.jpeg)
+
+1.  **Stabilizing and preparing for the next row:** Place base plates on
+    top of all cubes except 2.2. Instead, add the torch holder on top of
+    the cube on position 2.2.
+
+![](./IMAGES/smartphonemicroscope/image9.jpeg)
+
+1.  **Assemble the stage unit:** Take the manual z-stage with the
+    objective insert and place an empty cube around the objective
+    insert. Add two base plates on top of the two cubes for
+    stabilization like in the images below. Screw in your
+    infinity-corrected objective of choice.
+
+![](./IMAGES/smartphonemicroscope/image10.jpeg)
+
+![](./IMAGES/smartphonemicroscope/image11.jpeg)
+
+![](./IMAGES/smartphonemicroscope/image12.jpeg)
+
+
+1.  **Adding the next row of components:** Now place the stage unit on
+    top of cubes 1 and 2.1 with the objective insert directly above the
+    mirror in position 1 like shown below. Stick the eyepiece on
+    position 3 and another empty cube on position 4.
+
+![](./IMAGES/smartphonemicroscope/image13.jpeg)
+
+
+7.  **Adding the sample holder and phone mount:** Place the sample
+    holder on top of the cube with the objective. Make sure that you
+    orient the magnetic clip in a way that the sample slide later does
+    not hit the focusing knob. Place the phone holder on the 4^th^
+    position: this fixes your phone in place, so that you don't lose
+    your field of view and you can adjust the focus and sample easily --
+    however this is optional.
+
+![](./IMAGES/smartphonemicroscope/image14.jpeg)
+
+1.  **Adding the sample, the light and detection unit (aka your
+    phone):** Place your sample slide in the magnetic clip with the
+    cover glass pointing towards the objective. Finally, put the torch
+    on the torch holder in position 2.2 and turn it on. Now you can
+    place your phone in the phone holder and adjust it until you see the
+    sample plane -- a bright spot.
+
+![](./IMAGES/smartphonemicroscope/image15.jpeg)
+
+1.  **Final touches:** Focus the sample with the focus knob and/or by
+    adjusting the height of the sample holder. The size of the
+    illuminated area depends on the collimation of the torch. You can
+    change the collimation by pulling or pushing the front part of the
+    torch.
+
+### Your Microscope can do Fluorescence AND Brightfield microscopy!
+
+Fluorescence microscopy is achieved by placing the torch on top of the
+dichroic mirror. The resulting image is the fluorescence overlayed
+with the brightfield image due to the room light entering the
+microscope from the top of the sample. If you cover the top of the
+sample, you are left with the pure fluorescence image. However, some
+reflections from room light entering other components might be seen as
+well. To avoid this, you can darken the room or cover all components.
+
+![](./IMAGES/smartphonemicroscope/image16.jpeg)
+
+Set-up with a cover on top of the sample holder.
+
+![](./IMAGES/smartphonemicroscope/image17.jpeg)
+
+![](./IMAGES/smartphonemicroscope/image18.jpeg)
+
+
+Fibers coloured with a highlighter pen. Left: Fluorescence image
+overlayed with the brightfield image created from room light. Right:
+Fluorescence image when the top of the sample holder is covered from
+light.
+
+To achieve the typical brightfield image, place the torch with the
+torch insert on top of the sample holder. If you don't want to move
+the torch holder, you can place a diffuser or a piece of rigid plastic
+on the sample holder on which the torch can stand but transmits the
+light.
+
+![](./IMAGES/smartphonemicroscope/image19.jpeg)
+
+![](./IMAGES/smartphonemicroscope/image20.jpeg)
+
+
+Brightfield image of fibers. Left: without any torch, just from the
+room light. Right: with the torch on top of the diffuser on the sample
+holder.
+
+
+## Results 
+
+![](./IMAGES/Folie1.png)
+
+![](./IMAGES/Folie2.png)
+
+![](./IMAGES/Folie3.png)