diff --git a/pytest_django/asserts.py b/pytest_django/asserts.py
index 14741066..e64cebc1 100644
--- a/pytest_django/asserts.py
+++ b/pytest_django/asserts.py
@@ -160,6 +160,13 @@ def assertInHTML(
         msg_prefix: str = ...,
     ) -> None: ...
 
+    # Added in Django 5.1.
+    def assertNotInHTML(
+        needle: str,
+        haystack: str,
+        msg_prefix: str = ...,
+    ) -> None: ...
+
     def assertJSONEqual(
         raw: str,
         expected_data: Any,