diff --git a/CHANGELOG.md b/CHANGELOG.md
index 8848d22fd9..cf5655cded 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -29,6 +29,8 @@
 
 #### Deprecations
 
+- Deprecated warnings will be triggered when using snowpark-python with Python 3.9. For more details, please refer to https://docs.snowflake.com/en/developer-guide/python-runtime-support-policy.
+
 #### Dependency Updates
 
 #### Improvements
diff --git a/src/snowflake/snowpark/__init__.py b/src/snowflake/snowpark/__init__.py
index dd9fa994d3..1f6633086c 100644
--- a/src/snowflake/snowpark/__init__.py
+++ b/src/snowflake/snowpark/__init__.py
@@ -91,10 +91,24 @@
     "the Snowflake team. For more details, please refer "
     "to https://docs.snowflake.com/en/developer-guide/python-runtime-support-policy."
 )
+_deprecation_warning_msg_for_3_9 = (
+    "Python Runtime 3.9 reached its End-Of-Life (EOL) in October 2025, there will be no further bug fixes "
+    "or security updates for this runtime. We recommend that you upgrade your existing Python 3.9 objects to "
+    "Python 3.10, 3.11, 3.12 or 3.13. Please note that end of support does not impact execution, "
+    "and you will still be able to update and invoke existing objects. "
+    "However, they will be running on an unsupported runtime which will no longer be maintained or patched by "
+    "the Snowflake team. For more details, please refer "
+    "to https://docs.snowflake.com/en/developer-guide/python-runtime-support-policy."
+)
+
 warnings.filterwarnings(
     "once",  # ensure the warning is only shown once to avoid warning explosion
     message=_deprecation_warning_msg,
 )
+warnings.filterwarnings(
+    "once",
+    message=_deprecation_warning_msg_for_3_9,
+)
 
 if sys.version_info.major == 3 and sys.version_info.minor == 8:
     warnings.warn(
@@ -102,3 +116,10 @@
         category=DeprecationWarning,
         stacklevel=2,
     )
+
+if sys.version_info.major == 3 and sys.version_info.minor == 9:
+    warnings.warn(
+        _deprecation_warning_msg_for_3_9,
+        category=DeprecationWarning,
+        stacklevel=2,
+    )
diff --git a/tests/unit/test_deprecation.py b/tests/unit/test_deprecation.py
index 64e11a202c..fb01542e1f 100644
--- a/tests/unit/test_deprecation.py
+++ b/tests/unit/test_deprecation.py
@@ -25,3 +25,21 @@ def test_py38_deprecation():
     with pytest.warns(None) as record:
         importlib.import_module("snowflake.snowpark")
     assert len(record) == 0
+
+
+def test_py39_deprecation():
+    if not (sys.version_info.major == 3 and sys.version_info.minor == 9):
+        pytest.skip("This test is for Python 3.9 only")
+
+    with pytest.warns(
+        DeprecationWarning,
+        match="Python Runtime 3.9 reached its End-Of-Life",
+    ) as record:
+        importlib.reload(snowflake.snowpark)
+
+    assert len(record) == 1
+
+    # import again won't trigger the warning
+    with pytest.warns(None) as record:
+        importlib.import_module("snowflake.snowpark")
+    assert len(record) == 0